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Plasmid#55896PurposeLocalization: Membrane, Excitation: 559, Emission: 600DepositorInsertFarnesyl (HRAS Human)
UseTagsmRuby2ExpressionMammalianMutationencodes final 20 aa of NM_001130442.1PromoterCMVAvailable sinceApril 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
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TRE-UBC-hCD59-T2A-GFP
Plasmid#205460Purposelentiviral plasmid for expression of human CD59DepositorInserthCD59 (CD59 Human)
UseTagsExpressionMammalianMutationPromoterUBCAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
ABE8.13-m
Plasmid#136296Purposeexpresses ABE8.13-m in mammalian cellsDepositorInsertABE8.13-m
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pFastBac (GST-SNAP-Shp2)
Plasmid#177897PurposeBaculo expression of GST tag fused to SNAP tag and human Shp2DepositorInsertShp2 (PTPN11 Human)
UseTagsTEV-GST-PreScission cut site-SNAP tagExpressionInsectMutationPromoterPolyhedrinAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-CMV-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP
Plasmid#188772PurposedCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)DepositorInsertdCas9-XTEN80-KRAB(Kox1)
UseLentiviralTagsHA-2xNLS-XTEN80(linker)-KRAB(Kox1)ExpressionMutationPromoterCMVAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pT7-7 asyn G51D
Plasmid#105747PurposeBacterial expression of mutant human alpha synucleinDepositorInsertalpha-synuclein G51D (SNCA Human)
UseTagsExpressionBacterialMutationG51DPromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pCAGGS-PHFF
Plasmid#100280PurposeExpression vector for phycocyanobilin (PCB) synthesis in mammalian cells.DepositorInsertMTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
UseTagsFLAG, T7 and Mitochondria-targeting sequence (MTS…ExpressionMammalianMutationAll genes are codon-optimized for expression in h…PromoterCAG promoterAvailable sinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pHR-UCOE-SFFV-Zim3-dCas9-loxP-P2A-EGFP-loxP
Plasmid#188774PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionExpressionMutationPromoterSFFVAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMinDis.X513-iNicSnFR3a-(CC93)-PM
Plasmid#125122PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotineDepositorInsertiNicSnFR3a-(CC93)-PM
UseTagsIg-K-Chain and membrane anchoring sequence-PDGFRExpressionMammalianMutationNAPromoterT7Available sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5_FKBP25-V5-APEX2
Plasmid#83418PurposeExpressed FKBP25/FKBP3-V5-APEX2 in mammalian cellDepositorInsertPeptidyl-prolyl cis-trans isomerase FKBP3 (FKBP3 Human)
UseTagsAPEX2 and V5ExpressionMammalianMutationPromoterCMV-FAvailable sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
LZRS ERTm RasV12
Plasmid#21199DepositorUseRetroviralTagsExpressionMutationEsr1 G257R; HRasG12V ERTm bx3PromoterAvailable sinceJuly 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.SV40.THBS1-deltaTSR1-HA.SV40(polyA)
Plasmid#156162PurposeExpresses murine Thrombospondin-1 (THBS1) with deletion (AA 383-548) and HA-tag at C-terminusDepositorInsertThrombospondin-1 (Thbs1 Mouse)
UseAAVTagsHA-tagExpressionMammalianMutationTHBS1 with deletion of amino acid residues 383-548PromoterCMVAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-SARS-CoV-2-S-RBD-8his-N501Y
Plasmid#164845PurposeMammalian expression plasmid for RBD of SARS-CoV-2 (N501Y mutant) with 8his tagDepositorInsertSecreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
UseTags8his tag and HA leader sequenceExpressionMammalianMutationCodon-optimized for human cell expression. Has N5…PromoterCMVAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3HA-AR-LBD
Plasmid#89111Purposemammalian expression of human androgen receptor ligand binding domain amino acid residues 624-919 with N-terminal double HA tagDepositorInserthAR ligand binding domain (AR Human)
UseTags2xHAExpressionMammalianMutationcontains Ligand Binding Domain (624-919)PromoterCMVAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EFS-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP
Plasmid#188771PurposedCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)DepositorInsertdCas9-XTEN80-KRAB(Kox1)
UseLentiviralTagsHA-2xNLS-XTEN80(linker)-KRAB(Kox1)ExpressionMutationPromoterEFSAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
HTRA1_PDZ_1
Plasmid#103912PurposeProtein expression and purification of HTRA1 PDZ domainDepositorInsertHTRA1 (HTRA1 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
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ABE8.17-d
Plasmid#136299Purposeexpresses ABE8.17-d in mammalian cellsDepositorInsertABE8.20-d
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
DLG4_PDZ_3
Plasmid#103953PurposeProtein expression and purification of PSD95 PDZ3 domainDepositorInsertDLG4 (DLG4 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOTTC810 - pAAV-EF1a-CRTsigpep-GCaMP3 (D324G+D360G+397G+D435G 10.19)-KDEL
Plasmid#63885PurposeAn AAV packaging vector that expresses ER-retained low-affinity GCaMP3 variant under control of the EF1a promoter.DepositorInsertER-localized low-affinity GCaMP3(10.19)
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLX311 HRAS
Plasmid#117749PurposeOpen reading frame vector encoding HRASDepositorInsertRASH1 (HRAS Human)
UseLentiviralTagsV5ExpressionMammalianMutationClosed vector so will not read through the V5 reg…PromoterEF1aAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pLJM1-FH-AGO2-R438E
Plasmid#91984PurposeExpresses FLAG-HA-AGO2 (R438E)DepositorInsertAGO2 (AGO2 Human)
UseLentiviralTagsFLAG-HAExpressionMammalianMutationR438EPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOTTC592 - pCMV Mito-iRFP
Plasmid#59135PurposeA plasmid that express mitochondrial-targeted iRFP under the CMV promoter.DepositorInsertMitochondria-localized Infrared Fluorescent Protein
UseTagsMitochondria-targeting sequenceExpressionMammalianMutationPromoterCMV-IEAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOTTC591 - pCMV Mem-iRFP
Plasmid#59134PurposeA plasmid that express membrane-targeted iRFP under the CMV promoter.DepositorInsertMembrane-localized Infrared Fluorescent Protein
UseTagsPalmitylation signalExpressionMammalianMutationPromoterCMV-IEAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
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AFDN_PDZ_1
Plasmid#103938PurposeProtein expression and purification of TRITHORAX PDZ domainDepositorInsertAFDN (AFDN Human)
UseTagsGSTExpressionBacterialMutationcontains AA983-1110 of AFDNPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
plenti-UbcP-IKZF1-V2-3xHA-pGK-Pur
Plasmid#69051Purposelentiviral expression of IKZF1 with HA tagDepositorInsertIKZF1 (IKZF1 Human)
UseLentiviralTagsHAExpressionMammalianMutationsplice variant 2PromoterUBCAvailable sinceNov. 1, 2017AvailabilityAcademic Institutions and Nonprofits only