We narrowed to 4,941 results for: puc

1. TC1078-30

   Plasmid

   #164878

   Purpose

   30bp(mismatch17) cas13d gRNA for 1405 editing

   Depositor

   Tian Chi

   Article

   Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:

   Insert

   gRNA for TC1405
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGGF012

   Plasmid

   #48849

   Purpose

   Provides a sulfadiazine resistance cassette as GreenGate module.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Insert

   pMAS::SulfadiazineR::t35S
   

   Use

   Golden gate compatible cloning vector

   Tags

   Expression

   Mutation

   internal BsaI recognition sites in SulfR removed …

   Promoter

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PB-T3G-ERP2

   Plasmid

   #175188

   Purpose

   All-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Oct. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCas49

   Plasmid

   #82391

   Purpose

   rbcL-sfGFP expressed from PccmK2 promoter with Gmr in glpK

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   rbcL-GFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   PccmK2

   Available since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. qTAG-N-Puro-moxGFP

   Plasmid

   #207688

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-moxGFP cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pZH516

   Plasmid

   #102669

   Purpose

   Constitutive expression of GFPmut2; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

   Depositor

   Zach Hensel

   Article

   Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

   Insert

   GFPmut2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAK008

   Plasmid

   #128225

   Purpose

   classic sgRNA backbone for tRNA-gRNA array, Position 2

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   classic sgRNA backbone for tRNA-gRNA array, Position 2
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAK009

   Plasmid

   #128226

   Purpose

   classic sgRNA backbone for tRNA-gRNA array, Position 3

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   classic sgRNA backbone for tRNA-gRNA array, Position 3
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTetQCas-8+IS186

   Plasmid

   #170636

   Purpose

   Expression of CRISPR-associated transposases

   Depositor

   Sheng Yang

   Article

   Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

   Insert

   VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(8+IS186 array)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRE57I-GNAc

    Plasmid

    #170637

    Purpose

    DNA donor for transposition

    Depositor

    Sheng Yang

    Article

    Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

    Insert

    Mini-Tn6677, GNAc cassette, lacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pZH514

    Plasmid

    #102667

    Purpose

    Constitutive expression of GFPmut2; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCbeta EV (PKA catalytic subunit Cbeta)

    Plasmid

    #15311

    Depositor

    Stanley McKnight

    Article

    Uhler et al J Biol Chem. 1987 Nov 5. 262(31):15202-7

    Insert

    PRKACB (Prkacb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pGGE001

    Plasmid

    #48839

    Purpose

    Provides the rbcS terminator as GreenGate module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    rbcS terminator
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMIN-VS1

    Plasmid

    #197783

    Purpose

    Level 2 destination vector: pMIN-VS1 backbone

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAK005

    Plasmid

    #128218

    Purpose

    improved sgRNA backbone for tRNA-gRNA array, Position 5

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for tRNA-gRNA array, Position 5
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAK003

    Plasmid

    #128216

    Purpose

    improved sgRNA backbone for tRNA-gRNA array, Position 3

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for tRNA-gRNA array, Position 3
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAK004

    Plasmid

    #128217

    Purpose

    improved sgRNA backbone for tRNA-gRNA array, Position 4

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for tRNA-gRNA array, Position 4
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAK006

    Plasmid

    #128219

    Purpose

    improved sgRNA backbone for tRNA-gRNA array, Position 6

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for tRNA-gRNA array, Position 6
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAK002

    Plasmid

    #128215

    Purpose

    improved sgRNA backbone for tRNA-gRNA array, Position 2

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for tRNA-gRNA array, Position 2
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LI F-G Spec

    Plasmid

    #104521

    Purpose

    LI resistance gene module

    Depositor

    Thomas Lahaye

    Article

    Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

    Insert

    Spectinomycin resistance gene
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge