Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 70,703 results for: LAS

Showing: 4591 - 4620 of 70703 results

  7. pAAV hSyn1 CaRhGC wt T2A tDimer

    Plasmid
    #101720
    Purpose
    humanized, Neuron-specific promoter driven expression of the rhodopsin guanylyl cyclase from Catenaria anguillulae with a neuron-specific promoter. Useful for raising intracellular cAMP close to the m
    Depositor
    Thomas Oertner
    Article
    Scheib et al Nat Commun. 2018 May 24;9(1):2046. doi:
    Inserts
    CatRhGC
    red fluorescent protein
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn1 and ribosomal skip sequence T2A
    Available since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    101720-plasmid-map-sequence-id-201303

    Enlarge

  14. pCEP4-HLA-c-myc-optiT1R3 ECD-MHC

    Plasmid
    #113961
    Purpose
    mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 extracellular domain with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain fused to a canonical
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R3 (TAS1R3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    residues 21-563 fused to a transmembrane tether
    Promoter
    cmv
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113961-plasmid-map-sequence-id-215291

    Enlarge

  15. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

    Plasmid
    #171090
    Purpose
    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Sth1 dCas9
    Chloramphenicol acetyltransferase
    TagBFP
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…
    Expression
    Mutation
    D9A, H599A
    Promoter
    TUB1 (Toxoplasma gondii), transcriptionally linke…
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171090-plasmid-map-sequence-id-335786

    Enlarge

  18. pHcRed-NPM1cOligodN243-C1

    Plasmid
    #131847
    Purpose
    Expresses HcRed-NPM1c (cytoplasmic mutant, human) with the oligo dimerization domain attached to the last 50 aminoacids in mammalian cells through transient transfection
    Depositor
    Mark Minden
    Article
    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:
    Insert
    NPM1 (NPM1 Human)
    Use
    Tags
    HcRed
    Expression
    Mammalian
    Mutation
    This is a deletion contruct of the cytoplasmic m…
    Promoter
    Available since
    May 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    131847-plasmid-map-sequence-id-264401

    Enlarge

  19. pHcRed-NPM1cNLSdN243-C1

    Plasmid
    #131848
    Purpose
    Expresses HcRed-NPM1c (cytoplasmic mutant, human) with the NLS domain (aa. 121-195) attached to the last 50 aminoacids in mammalian cells through transient transfection
    Depositor
    Mark Minden
    Article
    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:
    Insert
    NPM1 (NPM1 Human)
    Use
    Tags
    HcRed
    Expression
    Mammalian
    Mutation
    This is a deletion contruct of the cytoplasmic m…
    Promoter
    Available since
    May 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    131848-plasmid-map-sequence-id-264370

    Enlarge

  23. pAAV hSyn INTRON mScarlet-CAAX2 WPRE

    Plasmid
    #236232
    Purpose
    AAV expression of a fluorescent marker, mScarletI, fused to the plasma membrane targeting sequence CAAX2
    Depositor
    Jennifer Lippincott-Schwartz
    Article
    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do
    Insert
    mScarletI fused to the plasma membrane targeting sequence CAAX2
    Use
    AAV
    Tags
    mScarletI
    Expression
    Mutation
    Promoter
    human Synapsin 1
    Available since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    236232-plasmid-map-sequence-id-471081

    Enlarge

  25. pSc1-DD

    Plasmid
    #80439
    Purpose
    Expresses eGFP with ecDHFR along with an U6 promoter driven gRNA which cleaves the plasmid in-cell
    Depositor
    Ervin Welker
    Article
    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare
    Inserts
    sp Cas9 gRNA
    eGFP
    Use
    CRISPR
    Tags
    E. coli dihydrofolate reductase
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6
    Available since
    Aug. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80439-plasmid-map-sequence-id-151455

    Enlarge

  29. pSc2

    Plasmid
    #80437
    Purpose
    Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell
    Depositor
    Ervin Welker
    Article
    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare
    Inserts
    sp Cas9 gRNA
    eGFP
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6
    Available since
    Sept. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    80437-plasmid-map-sequence-id-151450

    Enlarge

Showing: 4591 - 4620 of 70703 results