We narrowed to 17,688 results for: SHE

1. pET MBP TEV expression vector with BrioBrick polypromoter restriction sites (14-Q)

   Plasmid

   #48311

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   MBP

   Expression

   Bacterial

   Available Since

   Sept. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-NT-3-mCherry

   Plasmid

   #190201

   Purpose

   To express the mouse NT-3 tagged with the FP mCherry

   Depositor

   Pantelis Tsoulfas

   Insert

   NT-3
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   The codon were optimized for better expression

   Promoter

   CMV

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pB-CAG-PE2-NatMX

   Plasmid

   #158567

   Purpose

   Piggybac vector that allows for the generation of PE2-expressing stable cell lines

   Depositor

   Alejandro Chavez

   Insert

   PE2
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Sperm whale Mb 64Y68F in pVP80K

   Plasmid

   #86956

   Purpose

   Increases expression of sperm whale myoglobin protein for making apomyoglobin as a reagent for measuring rates of hemin dissociation (as opposed to previous construct in pUC).

   Depositor

   John Olson

   Insert

   Mb 64Y68F
   

   Expression

   Bacterial

   Mutation

   64Y68F

   Promoter

   T5

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK615

   Plasmid

   #71699

   Purpose

   Produces E. coli thioredoxin with a C-terminal His6 tag (EcTrxAH6)

   Depositor

   T. J. Kappock

   Insert

   thioredoxin
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUA97

   Plasmid

   #69340

   Purpose

   It encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategy

   Depositor

   Oliver Hobert

   Insert

   ceBFP SEC 3xFlag
   

   Tags

   3xFlag and ceBFP (mTagBFP)

   Expression

   Worm

   Mutation

   sqt-1(e1350)

   Available Since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. mouse ptc probe M2-3

   Plasmid

   #58701

   Purpose

   makes mouse ptc probe for in situ hybridization

   Depositor

   Matthew Scott

   Insert

   ptc (Ptch1 Mouse)
   

   Use

   In situ probe

   Expression

   Bacterial

   Mutation

   only EcoRI fragment (5' end)

   Promoter

   T7

   Available Since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pAc5.1B-EGFP-DmRRP40

   Plasmid

   #65797

   Purpose

   expresses EGFP-tagged RRP40 in Drosophila cells

   Depositor

   Elisa Izaurralde

   Insert

   RRP40 (Rrp40 Fly)
   

   Use

   Drosophila expression

   Tags

   EGFP

   Promoter

   Actin 5C

   Available Since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLXIN2-RFP

   Plasmid

   #99205

   Purpose

   RFP expressing bicistronic retroviral vector

   Depositor

   Saroj Mathupala

   Insert

   Red fluorescent protein
   

   Use

   Retroviral; Bicistronic retroviral expression vec…

   Expression

   Mammalian

   Mutation

   The dsRed cDNA was PCR amplified off AddGene plas…

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLIX403-EBFP2-ccdB-Blast

    Plasmid

    #158557

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EBFP2

    Expression

    Mammalian

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKSB- 3xP3-mTurqSV40 GGGG AAGA

    Plasmid

    #174533

    Purpose

    3xP3-mTurquoise fluorescence marker cassette for gene knock-in in mosquitoes

    Depositor

    Eric Marois

    Insert

    3xP3-mTurquoise BsaI cassette
    

    Expression

    Insect

    Promoter

    3xP3

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKT-PDGFRa-IRES-Katushka

    Plasmid

    #133081

    Purpose

    Expresses PDGFRa receptor and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Insert

    PDGFRa (Pdgfra Mouse)
    

    Expression

    Mammalian

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-ePEG-LINE1-loxPsym

    Plasmid

    #216866

    Purpose

    Lentiviral guide RNA vector to insert the loxPsym sequence into LINE1 elements

    Depositor

    Leopold Parts

    Insert

    spacerLINE1_cr772scaffold_PBS-loxPsm-HA-tevopreQ
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHH0103 NEDD4 WW domain #3

    Plasmid

    #104210

    Purpose

    Bacterial expression of WW domain #3 from NEDD4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4 WW domain #3 (NEDD4 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHH0103 NEDD4L WW domain #2

    Plasmid

    #104205

    Purpose

    Bacterial expression of WW domain #2 from NEDD4L

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4L WW domain #2 (NEDD4L Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJM104: pTrc RFP on pCM66T

    Plasmid

    #74745

    Purpose

    pCM66T with promoter pTrc driving RFP expression (maxRFP = BBa_E1010)

    Depositor

    Mary Lidstrom

    Insert

    RFP (BioBrick part number BBa_E1010)
    

    Promoter

    pTrc

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGAL-flp(H305L)-NAT

    Plasmid

    #160380

    Purpose

    overexpression of Flp recombinase step-arrest mutant in yeast

    Depositor

    John McCusker

    Insert

    FLP1
    

    Expression

    Yeast

    Mutation

    H305L

    Promoter

    GAL

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHH0103 NEDD4 WW domain #1

    Plasmid

    #104208

    Purpose

    Bacterial expression of WW domain #1 from NEDD4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4 WW domain #1 (NEDD4 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pExp-PpiB

    Plasmid

    #112572

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and PpiB (peptidyl-prolyl cis-trans isomerase B from E. coli) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Tags

    8xHis-PpiB and Strep tag

    Expression

    Bacterial

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDTA-5

    Plasmid

    #128845

    Purpose

    Gateway-compatible p15A origin plasmid with a DTA negative selection cassette for targeting vector construction, I-CeuI, low copy number

    Depositor

    Mario Capecchi

    Type

    Empty backbone

    Use

    Mouse Targeting

    Expression

    Mammalian

    Available Since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pHH0103 NEDD4 WW domain #4

    Plasmid

    #104211

    Purpose

    Bacterial expression of WW domain #4 from NEDD4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4 WW domain #4 (NEDD4 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. CbSBS-Flag/TRRAP(S722F)

    Plasmid

    #32105

    Depositor

    Yardena Samuels

    Insert

    Transformation/transcription domain-associated protein (S722F) (TRRAP Human)
    

    Tags

    Flag 1x

    Expression

    Mammalian

    Mutation

    Changes serine 722 to Phe

    Promoter

    CMV

    Available Since

    Nov. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

23. AAVS1_Puro_H2BK_mNG_hSLBP(18-108)

    Plasmid

    #191529

    Purpose

    Donor for targeted integration of mNG_hSLBP(18-108) probe to the AAVS1 safe harbor locus

    Depositor

    Yannick Doyon

    Insert

    H2BK_mNG_hSLBP(18-108)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    mNeonGreen (mNG)

    Expression

    Mammalian

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. PB CAG-sfGFP-iCre-ERT2

    Plasmid

    #137856

    Purpose

    CAG-sfGFP-iCre-ERT2 piggyBac transposon

    Depositor

    Mario Capecchi

    Insert

    sfGFP-iCre-ERT2
    

    Use

    Cre/Lox; Piggybac

    Expression

    Mammalian

    Available Since

    Nov. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. pKSB- 3xP3-GFPsv40 GGGG AAGA

    Plasmid

    #174531

    Purpose

    3xP3-GFP fluorescence marker cassette for gene knock-in in mosquitoes

    Depositor

    Eric Marois

    Insert

    3xP3-GFP BsaI cassette
    

    Expression

    Insect

    Promoter

    3xP3

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. 1781_pAAV-U6-Ai9-Sa-gRNA1-CB-SACas9-HA-OLLAS-spA_C

    Plasmid

    #135979

    Purpose

    gRNA against the left loxP site of the Ai9 allele

    Depositor

    William Lagor

    Insert

    Ai9 gRNA1
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    HA

    Promoter

    U6

    Available Since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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27. pJK633

    Plasmid

    #72397

    Purpose

    Produces Escherichia coli aminoimidazole ribonucleotide synthetase, N-terminal His6 tag (H6EcPurM)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole synthetase
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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28. pcDNA3.1 myc-PITPalpha

    Plasmid

    #58277

    Purpose

    mammalian expression of human PITP alpha

    Depositor

    Shamshad Cockcroft

    Insert

    PITP alpha (PITPNA Human)
    

    Tags

    myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

29. 4IJD

    Plasmid

    #51328

    Depositor

    Cheryl Arrowsmith

    Insert

    4IJD (PRDM9 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Truncation: See Comments

    Promoter

    T7

    Available Since

    Feb. 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

30. Preprotachykinin A

    Plasmid

    #130664

    Purpose

    Expresses human Preprotachykinin A in mammalian cells

    Depositor

    Michael Kressel

    Insert

    Preprotachykinin A (TAC1 Human)
    

    Tags

    Fusion Red

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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