We narrowed to 23,164 results for: his
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Plasmid#199772PurposeExpresses split-intein fused MSP1E3D1 for circularization during expression (external His-Tag)DepositorInsertcMSP1E3D1
TagsHis6-TagExpressionBacterialMutationExtended cMSP1D1; external His-Tags flanking the …Available SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPD005 pcDNA Golden Gate
Plasmid#138749PurposeVector used to express most COMET components, modified from pcDNA3.1/HygroR(+) for BsaI/BpiI Golden Gate compatibility and to lack HygroR.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionMammalianAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD304 VPR-ZF43
Plasmid#138840PurposeConstitutive expression of VPR-ZF43 under the CMV promoter.DepositorInsertVPR-ZF43
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-cMSPdH45
Plasmid#199767PurposeExpresses split-intein fused MSPdH45 for circularization during expression (internal His-Tag)DepositorInsertcMSPdH45
TagsHis6-TagExpressionBacterialMutationinternal His-TagAvailable SinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE30-mBFP
Plasmid#112682PurposeExpression of NADPH-dependent blue fluorescent proteinDepositorInsertHis-mBFP
TagsHis tagExpressionBacterialPromoterT5Available SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO-TtAgo
Plasmid#210191PurposeBacterial expression of the His- and SUMO-tagged Argonaute protein of Thermus thermophilusDepositorInsertTtAgo (TT_P0026 Thermus thermophilus)
Tags6x His tag and SUMO tagExpressionBacterialPromoterT7Available SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
PARP9-c020
Plasmid#173097PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
PARP9-c024
Plasmid#173096PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD189 VP16-ZF37
Plasmid#138759PurposeConstitutive expression of VP16-ZF37 under the CMV promoter.DepositorInsertVP16-ZF37
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Parp14-c012
Plasmid#173099PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Parp14-c010
Plasmid#173100PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD528 VP16-ZF21
Plasmid#138757PurposeConstitutive expression of VP16-ZF21 under the CMV promoter.DepositorInsertVP16-ZF21
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM456 EF1alpha EBFP2-P2A-BlastR (TUPV7)
Plasmid#139236PurposemMoClo TUPV7, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCSDepositorInsertEBFP2-P2A-Blasticidin S Deaminase
UseSynthetic BiologyExpressionMammalianPromoterEF1alphaAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
PARP15-c020
Plasmid#173101PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
PARP15-c021
Plasmid#173102PurposeExpression of recombinant fusion protein in E. coliDepositorInsertPARP15 (PARP15 Human)
TagsmCeruleanExpressionBacterialMutationG287-N470, codon optimizedAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Parp14-c011
Plasmid#173098PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN154
Plasmid#86718PurposeFLP/Frt dual color reporter for C. elegansDepositorInsertPhsp16.41::FRT::mCh::his-58::FRT::gfp::his-58
ExpressionWormAvailable SinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPD100 VP16-ZF43
Plasmid#138761PurposeConstitutive expression of VP16-ZF43 under the CMV promoter.DepositorInsertVP16-ZF43
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
PCRT7 tetR pLtetO MBP-MEK1 XX Amp
Plasmid#53225Purposehuman MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesisDepositorInsertMEK1 (MAP2K1 Human)
UsePhosphoprotein synthesisTagsHIS and MBPExpressionBacterialMutationCodon optimized for E.coli, both codons for Ser21…PromoterpLtetOAvailable SinceMay 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
VV001: WT Arch-3 in pET28b
Plasmid#58487PurposeExpresses WT Archaerhodopsin-3 (with 6x C-terminal His tag) in E. coliDepositorInsertArchaerhodopsin-3 (with C-terminal His tag)
ExpressionBacterialPromoterT7Available SinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPD536 VP16-ZF150
Plasmid#138769PurposeConstitutive expression of VP16-ZF150 under the CMV promoter.DepositorInsertVP16-ZF150
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD538 VP16-ZF172
Plasmid#138772PurposeConstitutive expression of VP16-ZF172 under the CMV promoter.DepositorInsertVP16-ZF172
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-Rsn2
Plasmid#61686Purposebacterial expression of ranaspumin 2 as a His-tagged fusion proteinDepositorInsertRanaspumin-2
TagsHisExpressionBacterialPromoterT7Available SinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJCC_039 FnCas12a
Plasmid#171666PurposeFor bacterial expression of FnCas12a with a C-terminal sorting motif and His tagDepositorInsertFnCas12a
Tags6X His and sortase A sorting motifExpressionBacterialAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD539 VP16-ZF173
Plasmid#138773PurposeConstitutive expression of VP16-ZF173 under the CMV promoter.DepositorInsertVP16-ZF173
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD526 VP16-ZF13
Plasmid#138755PurposeConstitutive expression of VP16-ZF13 under the CMV promoter.DepositorInsertVP16-ZF13
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWaldo-GFPd_PepTSt
Plasmid#58333PurposeExpresses the POT family transporter from Streptococcus thermophilus in E. coli as a C-terminally tagged GFP his tagged proteinDepositorInsertPepTSt
TagsGFP-HisExpressionBacterialPromoterT7Available SinceSept. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPD1028 ZF43x6-Compact(SV40Min)_EYFP
Plasmid#138912PurposeEYFP reporter vector for the ZF43x6-Compact(overlapping ZF37) promoter with a SV40 minimal promoterDepositorInsertZF43x6-Compact(SV40min)
UseSynthetic BiologyExpressionMammalianPromoterZF43x6-Compact(SV40min)Available SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTD-C_eYFPTwinStrep
Plasmid#45942Purposeexpresses E.coli codon optimized EYFP-Twin-Strep fusion proteinDepositorInsertsynthetic eYFP (codon usage adapted to E.coli K12)
TagsTwin-Strep-tagExpressionBacterialPromoterlacIq/PtrcAvailable SinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCoofy48
Plasmid#55186PurposeBacterial expression vector for parallel SLIC cloning containing N-terminal 6x His and GST tags. C-terminal tags can be added during SLIC.DepositorTypeEmpty backboneTags6x His, GST, His10-Stop-OneStrep-Stop-S-tag-Stop-…ExpressionBacterialPromoterT7Available SinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJCC_083 Cas12i1
Plasmid#171670PurposeFor bacterial expression of Cas12i1 with an N-terminal His-MBP tagDepositorInsertCas12i1
Tags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD057 VP64-ZF37
Plasmid#138834PurposeConstitutive expression of VP64-ZF37 under the CMV promoter.DepositorInsertVP64-ZF37
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD391 VPR-ZF37
Plasmid#138839PurposeConstitutive expression of VPR-ZF37 under the CMV promoter.DepositorInsertVPR-ZF37
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEQ1007
Plasmid#83938Purposeexpress human cytomegalovirus IRS1 with a C-terminal 6X his tag in eukaryotic cellsDepositorInsertIRS1
Tags6xHISExpressionMammalianMutationR833L in IRS1 (please see depositor comment)PromoterCMVAvailable SinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPD1029 ZF43x6-Compact(CMVMin)_EYFP
Plasmid#138913PurposeEYFP reporter vector for the ZF43x6-Compact(overlapping ZF37) promoter with a CMV minimal promoterDepositorInsertZF43x6-Compact(CMVmin)
UseSynthetic BiologyExpressionMammalianPromoterZF43x6-Compact(CMVmin)Available SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD159 dsRedExpress2-ZFi43
Plasmid#138799PurposeConstitutive expression of dsRedExpress2-ZFi43 under the CMV promoter.DepositorInsertdsRedExpress2-ZFi43
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
plIVMD156
Plasmid#160934PurposeIn vivo mRNA display Gateway Destination constructDepositorInsertMCP-Gateway cloning site-HIS tag, 3'UTR MS2 Stem Loop
TagsHIS tag, 3'UTR MS2 Stem LoopExpressionYeastPromoterMET25Available SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQLinkHD_EBFP2-LSSmOrange
Plasmid#118856Purposefluorescent protein expression with an N-terminal His-tagDepositorInsertEBFP2-LSSmOrange
TagsHis-tagExpressionBacterialMutationEBFP1.2-I128V/V150I/ D155V/V224R and for LSSmOra…Available SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Sc eRF1 (WT)
Plasmid#175413PurposeEncodes His-MBP-TEV-WT eRF1DepositorAvailable SinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOD8A-RGR-r10
Plasmid#74370PurposeThis plasmid constitutively expresses gRNA-r10 from an AHD1 promoter using the RGR design. Integrates in the W303 HIS locus and restores HIS function.DepositorArticleInsertRGR-r10
UseCRISPR and Synthetic BiologyExpressionYeastPromoterADH1Available SinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only