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We narrowed to 10,760 results for: ESP

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Showing: 7651 - 7700 of 10760 results

  3. BIC-Gag-DDX3

    Plasmid
    #233687
    Purpose
    Expresses a fusion between the Murine Leukemia Virus Gag polyprotein and the human DDX3 protein to produce virus-like particles loaded with DDX3 and deliver it to target cells.
    Depositor
    Emiliano Ricci
    Article
    Labaronne et al Nat Commun. 2025 Feb 18;16(1):1706. doi:
    Insert
    DEAD-box helicase 3 (DDX3X Human)
    Use
    Retroviral
    Tags
    Gag (Murine Leukemia Virus)
    Expression
    Mammalian
    Promoter
    hCMV
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    233687-plasmid-map-sequence-id-464903

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  4. pαH-S-GSAS-B.1.617.2.v1

    Plasmid
    #182575
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein Delta variant, furin side replaced by GSAS, 2 Proline at 986 and 987 plus T19R-G142D-del156/157-R158G-L452R-T478K-D614G-P681R-D950N
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6
    Insert
    Spike S-GSAS-B.1.617.2.v1 (T19R-G142D-Del156/157-R158G-L452R-T478K-D614G-P681R-D950N) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    182575-plasmid-map-sequence-id-353646

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  7. pQ-Elo-HB-C-A(400-772)

    Plasmid
    #171087
    Purpose
    Co-expresses of ΔN-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    Elongin B (ELOB Human)
    Elongin C (ELOC Human)
    Elongin A (ELOA Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Deleted N-term 26+399 amino acids for mutant expr…
    Promoter
    T5 promoter and T7 promoter
    Available Since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171087-plasmid-map-sequence-id-335508

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  12. pT7CFE1-Nhis-GST-CHA-YTH domain (YTHDC1, 317-532)

    Plasmid
    #102277
    Purpose
    For expression of His-GST-tagged human YTHDC1 YTH domain using mammalian cell-free protein expression system (1-step human high-yield mini IVT kit, cat. #88890, Thermo Fisher Scientific)
    Depositor
    Samie Jaffrey
    Article
    Kan et al Nat Commun. 2017 Jul 4;8:15737. doi: 10.
    Insert
    YTH domain of human YTHDC1 (317-532) (YTHDC1 Human)
    Use
    For mammalian cell-free protein expression system…
    Tags
    GST, HA, and HIS
    Promoter
    T7
    Available Since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    102277-plasmid-map-sequence-id-197159

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  23. pCMV-ABE8.20m-eVRQR-P2A-EGFP (LLH569)

    Plasmid
    #242656
    Purpose
    CMV promoter expression plasmid for human codon optimized ABE8.20 A-to-G base editor with neVRQR(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-ABE8.20m-VRQR-S55R-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8.20 mutations in TadA, eVRQR mutations in SpC…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242656-plasmid-map-sequence-id-488970

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  24. pCMV-ABE8e-eVRQR-P2A-EGFP (HES1425)

    Plasmid
    #242657
    Purpose
    CMV promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with neVRQR(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-ABE8e-SpCas9-VRQR(S55R)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA, eVRQR mutations in SpCas…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242657-plasmid-map-sequence-id-488990

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  25. pTwist-SARS-CoV-2 Δ18 B.1.1.529

    Plasmid
    #179907
    Purpose
    Encodes SARS-CoV-2 B.1.1.529 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Garcia-Beltran et al Cell. 2022 Jan 6. pii: S0092-8674(21)014
    Insert
    SARS-CoV-2 B.1.1.529 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    A67V; Δ69-70; T95I; G142D; Δ143-145; N211I; Δ212;…
    Promoter
    CAG
    Available Since
    Jan. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    179907-plasmid-map-sequence-id-351282

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  27. C-term AAV-SpCas9-VRQR-gRNA_entry (HES1477)

    Plasmid
    #242660
    Purpose
    CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and pU6 SpCas9 gRNA entry cassette
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-SpCas9_gRNA_entrycassette
    Use
    AAV and CRISPR
    Tags
    BPNLS
    Mutation
    VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…
    Promoter
    CBh
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242660-plasmid-map-sequence-id-489092

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  28. N-term AAV-ABE8e-SpCas9(S55R) (CA21)

    Plasmid
    #242659
    Purpose
    CBh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-SpCas9(S55R) - to be used with eVRQR constructs
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCBh-BPNLS(SV40)-TadA8e-gs-SpCas9(D10A;S55R)-[N-term]-NpuN-BPNLS(SV40)-WPRE-bGH_PA
    Use
    AAV and CRISPR
    Tags
    BPNLS and NpuN(intein)-BPNLS
    Mutation
    SpCas9(S55R)
    Promoter
    CBh
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242659-plasmid-map-sequence-id-489035

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  29. pαH-S-GSAS-2P-OMICRON

    Plasmid
    #180593
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6
    Insert
    Spike (S-GSAS-2P-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180593-plasmid-map-sequence-id-352871

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  30. pCMV-ABE8.8m-SpG-P2A-EGFP (BKS965)

    Plasmid
    #242652
    Purpose
    CMV promoter expression plasmid for human codon optimized ABE8.8m A-to-G base editor with SpG(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-ABE8.8m-SpG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8.8 mutations in TadA, SpG mutations in SpCas9…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242652-plasmid-map-sequence-id-488985

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  31. AiP11851-pAAV-hDLX-minBG-iCre-4X2C-WPRE3-BGHpA (Alias: CN1851)

    Plasmid
    #164450
    Purpose
    AAV vector for Cre expression in forebrain GABAergic interneurons. For use in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors.
    Depositor
    Allen Institute for Brain Science, Jonathan Ting
    Article
    Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)
    Has Service
    AAV PHP.eB
    Insert
    iCre
    Use
    AAV
    Promoter
    minBetaGlobin
    Available Since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    164450-plasmid-map-sequence-id-325648

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  32. pαH-S-GSAS-OMICRON

    Plasmid
    #180423
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6
    Insert
    Spike (S-GSAS-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    Feb. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    180423-plasmid-map-sequence-id-354264

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  33. pHis-TEV-Nsp15

    Plasmid
    #187656
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, wild-type SARS CoV-2 Nsp15 (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Jernigan et al Structure. 2022 Dec 30:S0969-2126(22)004
    Insert
    Nsp15 (N Severe acute respiratory syndrome coronavirus 2)
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7lac
    Available Since
    March 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    187656-plasmid-map-sequence-id-388768

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  41. pCMV-SpCas9-VRQR(+L1111R)-P2A-EGFP (LRF167)

    Plasmid
    #242673
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L1111R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(L1111R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + L1111R mutations in SpCas9(L1111R/D1135V/G…
    Promoter
    CMV and T7
    Available Since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    242673-plasmid-map-sequence-id-494379

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  42. pCMV-SpCas9-VRQR(+A1322R)-P2A-EGFP (LRF59)

    Plasmid
    #242679
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(A1322R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(A1322R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + A1322R mutations in SpCas9(D1135V/G1218R/A…
    Promoter
    CMV and T7
    Available Since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    242679-plasmid-map-sequence-id-494380

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  43. C-term AAV-eVRQR-ACTA2-gRNA-A4 (mouse) (LLH310)

    Plasmid
    #242662
    Purpose
    CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and mouse gRNA-A4
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-ACTA2_mouse_NGA_gRNA-pU6
    Use
    AAV and CRISPR
    Tags
    BPNLS
    Mutation
    VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…
    Promoter
    CBh
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242662-plasmid-map-sequence-id-489091

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  44. C-term AAV-eVRQR-ACTA2-gRNA-A4 (human) (LLH301)

    Plasmid
    #242661
    Purpose
    CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and human gRNA-A4
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-ACTA2_human_NGA_gRNA-pU6
    Use
    AAV and CRISPR
    Tags
    BPNLS
    Mutation
    VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…
    Promoter
    CBh
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242661-plasmid-map-sequence-id-489090

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  45. pCMV-SpCas9-VRQR(+R221K)-P2A-EGFP (LRF95)

    Plasmid
    #242666
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(R221K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(R221K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + R221K mutations in SpCas9(R221K/D1135V/G12…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242666-plasmid-map-sequence-id-489088

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  46. pCMV-eVRQR-P2A-EGFP (KAC1028)

    Plasmid
    #242663
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-eVRQR(S55R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-eVRQR(S55R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    eVRQR mutations in SpCas9(S55R/D1135V/G1218R/R133…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242663-plasmid-map-sequence-id-489032

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  47. pCMV-ABE8.8m-VRQR-P2A-EGFP (BKS971)

    Plasmid
    #242653
    Purpose
    CMV promoter expression plasmid for human codon optimized ABE8.8m A-to-G base editor with nVRQR(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-ABE8.8m-SpCas9-VRQR-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8.8 mutations in TadA, VRQR mutations in SpCas…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242653-plasmid-map-sequence-id-488991

    Enlarge

  48. pCMV-SpCas9-VRQR(+N394K)-P2A-EGFP (KAC956)

    Plasmid
    #242670
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(N394K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(N394K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + N394K mutations in SpCas9(N394K/D1135V/G12…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242670-plasmid-map-sequence-id-488989

    Enlarge

  49. pCMV-SpCas9-VRQR(+D269A)-P2A-EGFP (LRF64)

    Plasmid
    #242668
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(D269A)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(D269A)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + D269A mutations in SpCas9(D269A/D1135V/G12…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242668-plasmid-map-sequence-id-488987

    Enlarge

  50. pCMV-SpCas9-VRQR(+G366R)-P2A-EGFP (KAC1026)

    Plasmid
    #242669
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(G366R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(G366R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + G366R mutations in SpCas9(G366R/D1135V/G12…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242669-plasmid-map-sequence-id-488988

    Enlarge

Showing: 7651 - 7700 of 10760 results