Skip to main content
Addgene
Showing: 3061 - 3080 of 4038 results

  1. pUC19-POLBHR-eGFP

    Plasmid
    #176064
    Purpose
    Homology region +/- 800bp to the transcription start site of POLB, with EGFP inserted in-frame on the N-terminus of POLB, and a mutation in the PAM site used by POLBKO gRNA1 in POLB exon1
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    EGFP-PolB (POLB Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    pUC with endogenous SapI mutated out, PAM mutatio…
    Promoter
    N/A
    Available since
    Jan. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    176064-plasmid-map-sequence-id-352734

    Enlarge

  11. pIS1 HK1

    Plasmid
    #60794
    Purpose
    Luciferase reporter to measure miR-155 mediated differential repression. Contains HK1 3' UTR and wild-type miR-155 sites
    Depositor
    David Bartel
    Article
    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi
    Insert
    HK1 3'UTR and wild-type miR-155 binding site (HK1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    thymidine kinase (HSV-TK promoter)
    Available since
    Oct. 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    60794-plasmid-map-sequence-id-97804

    Enlarge

  12. pIS1 mHK1

    Plasmid
    #60795
    Purpose
    Luciferase reporter to measure miR-155 mediated differential repression. Contains HK1 3' UTR and mutated miR-155 sites
    Depositor
    David Bartel
    Article
    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi
    Insert
    HK1 3'UTR and mutated miR-155 binding site (HK1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    mutated miR-155 binding site
    Promoter
    thymidine kinase (HSV-TK promoter)
    Available since
    Oct. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    60795-plasmid-map-sequence-id-97806

    Enlarge

  18. pCDH-EF1-mVenus-p27K−

    Plasmid
    #176651
    Purpose
    It encodes an mVenus fused to a mutant p27K-, which lacks binding affinity to Cdk. This reporter helps to identify quiescent disseminated tumor cells (in G0 phase of cell cycle).
    Depositor
    Mohamed Bentires-Alj
    Article
    Correia et al Nature. 2021 Jun;594(7864):566-571. doi:
    Insert
    mVenus-p27K- (Cdkn1b Mouse)
    Use
    Lentiviral
    Tags
    mVenus
    Expression
    Mutation
    Mutant K- (lacks affinity to Cdk)
    Promoter
    Available since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    176651-plasmid-map-sequence-id-354255

    Enlarge

Showing: 3061 - 3080 of 4038 results