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pD(P+T)_T7N

Plasmid

#223855

Purpose

4G-cloning donor for bacterial promoter and terminator, N-terminal tag added

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

T7 promoter and terminator

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(P+T)_T7C

Plasmid

#223856

Purpose

4G-cloning donor for bacterial promoter and terminator, C-terminal tag added

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

T7 promoter and terminator

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(P+T)_T7

Plasmid

#223854

Purpose

4G-cloning donor for bacterial promoter and terminator, no tag added

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

T7 promoter and terminator

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His10-TS-3C

Plasmid

#223884

Purpose

4G-cloning donor for N-terminal His10-TwinStrep-3C tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

His10-TwinStrep-3C

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-SUMO

Plasmid

#223879

Purpose

4G-cloning donor for N-terminal His6-SUMO tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

His6-SUMO

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_GFP-TEV

Plasmid

#223881

Purpose

4G-cloning donor for N-terminal GFP-TEV tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

GFP-TEV

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_3C-GFP

Plasmid

#223888

Purpose

4G-cloning donor for C-terminal 3C-GFP tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

3C-GFP

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_3C-His8

Plasmid

#223887

Purpose

4G-cloning donor for C-terminal 3C-His8 tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

3C-His8

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_CPD-His10

Plasmid

#223890

Purpose

4G-cloning donor for C-terminal CPD-His10 tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

CPD-His10

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_5'alpha

Plasmid

#223863

Purpose

4G-cloning donor for 5'-Gibson overhang, alpha-sequence

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

5'alpha sequence and spacer

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_5'gamma

Plasmid

#223865

Purpose

4G-cloning donor for 5'-Gibson overhang, gamma-sequence

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

5'gamma sequence and spacer

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_5'beta

Plasmid

#223864

Purpose

4G-cloning donor for 5'-Gibson overhang, beta-sequence

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

5'beta sequence and spacer

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_5'delta

Plasmid

#223866

Purpose

4G-cloning donor for 5'-Gibson overhang, delta-sequence

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

5'delta sequence and spacer

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_3'beta

Plasmid

#223867

Purpose

4G-cloning donor for 3'-Gibson overhang, beta-sequence

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

3'beta sequence and spacer

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_3'gamma

Plasmid

#223868

Purpose

4G-cloning donor for 3'-Gibson overhang, gamma-sequence

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

3'gamma sequence and spacer

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_3'delta

Plasmid

#223869

Purpose

4G-cloning donor for 3'-Gibson overhang, delta-sequence

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

3'delta sequence and spacer

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAG426-GAL-Prepro-3x-Beta-Amyloid

Plasmid

#181730

Purpose

Galactose inducible expression of Prepro-3x-Beta-Amyloid

Depositor

Alejandro Chavez

Insert

Prepro-3x-Beta-Amyloid

Expression

Yeast

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pYTK057

Plasmid

#65164

Purpose

Encodes mTurquoise2 as a Type 4a part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

mTurquoise2

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK058

Plasmid

#65165

Purpose

Encodes Venus as a Type 4a part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

Venus

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK059

Plasmid

#65166

Purpose

Encodes mRuby2 as a Type 4a part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

mRuby2

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge