We narrowed to 17,934 results for: multi
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Plasmid#52381PurposeBAC recombineering generated construct, used as a reporter for OCT4 expressionDepositorInsertsExpressionMammalianMutationdeleted -3194 - -1768 upstream from OCT4 ATGPromoterOCT4Available sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pTUB1:OsTIR1-3FLAG, SAG1:CAT
Plasmid#87258PurposeTIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.DepositorInsertsTIR1
CAT
UseToxoplasma expressionTags3FLAGMutationCodon optimized for Toxoplasma gondii expressionPromoterTgSAG1 361 bp and TgTUB1 2717 bpAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHL-H1-ccdB-mEF1a-RiH
Plasmid#60601PurposeCloning vector for CRISPR-sgRNA (into the BamHI-EcoRI site), expresses RFP and hygromycin resistance gene.DepositorInsertsRed Fluorescent Protein
Hygromycin resistance gene
UseCRISPRExpressionMammalianAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTrig
Plasmid#104576PurposeCopy number inducible plasmid. Contains mini-F origin for maintenance and P1 lytic replication machinery under Lac promoter for copy increase.DepositorInsertPLac-repL
UseSynthetic BiologyAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8547 PB-PGK-dLbCpf1-2XNLS-miniVPR-mCherry-pA
Plasmid#140220PurposeConstitutive PGK driven expression of full length dCpf1 (Cas12a) -miniVPR activatorDepositorInsertdLbCpf1
UseCRISPRTagsminiVPR-mCherryExpressionMammalianMutationD832APromoterPGKAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYLsgRNA-OsU6c
Plasmid#66197Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorPromoterOsU6cAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mouse deltaPE Oct4-GFP transgenic reporter
Plasmid#52382PurposeBAC recombineering generated construct, used as a reporter for mouse Oct4 expressionDepositorInsertsExpressionMammalianMutationdeleted -300 - -1300bp fragment upstream from Octā¦PromoterOct4 and PGK+gb2Available sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUC19_HDRT_TRAC_CD19-CAR
Plasmid#183473PurposeEncodes for a homology-directed DNA repair template for the insertion of a second generation CD19-specific CAR into the TRAC geneDepositorAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE hSPC-eGFP-W
Plasmid#36450DepositorAvailable sinceMay 11, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNTI194
Plasmid#84266PurposeExpresses Avi-tagged RPL1 and humanized birADepositorInsertsTagsAvi tag, Flag, Gly/Ser linker, and TEV siteExpressionMammalianMutationR33A, A34E (*see below)PromoterCMV and P2A peptideAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-UCOE-SFFV-Zim3-dCas9-BFP
Plasmid#188776PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9-mTagBFP2
UseLentiviralTagsHA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusionPromoterSFFVAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5
Plasmid#97044PurposeExpresses LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells. *Please see notes below on P2A cleaving efficiency.DepositorInsertLigand Dependent Nuclear Receptor Corepressor, Runt-related transcription factor 1, ETS-related gene
UseLentiviralExpressionMammalianPromoterTet onAvailable sinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR
Plasmid#60225PurposeExpresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA targeting LacZ. AAV backbone.DepositorInsertssgRNA
Renilla luciferase
Cre recombinase
UseAAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targeā¦TagsCre-HA and Rluc-P2AExpressionMammalianPromoterEFS and U6Available sinceOct. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR iSH-YFP-PIF
Plasmid#50841PurposeOptogenetic PI3K activation system; cytoplasmic componentDepositorInsertsp85 iSH2
PIF6 1-100
UseLentiviral and Synthetic BiologyAvailable sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGGDestTol2LC-3sgRNA
Plasmid#64241Purposedestination vector for three U6x:sgRNA cassettesDepositorTypeEmpty backboneUseCRISPRAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGGDestTol2LC-2sgRNA
Plasmid#64240Purposedestination vector for two U6x:sgRNA cassettesDepositorTypeEmpty backboneUseCRISPRAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAC149-pCR8-dCas9VP160
Plasmid#48221PurposedCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expressionDepositorInsertdCas9(D10A;H840A) fusion with VP160 activation domain
UseCRISPR and Gateway CloningTagsHA Tag and VP160MutationD10A;H840A nuclease-deficientAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBad-LgBiT-PhoCl1-SmBiT-MBP
Plasmid#164034Purposeluciferase-based PhoCl screening constructDepositorPublicationInsertLgBiT-PhoCl1-SmBiT-MBP
TagsHis tag, T7 tag, Xpress TagExpressionBacterialPromoteraraBad promotorAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CB-iCre-P2A-tdTomato-T2A-Puro
Plasmid#72255PurposeExpress iCre, tdTomato and puromycin resistance gene (puromycin N-acetyl-transferase) under CB (chicken beta-actin + CMV enhancer) promoterDepositorInsertiCre-P2A-tdTomato-T2A-Puro
UseLentiviralExpressionMammalianPromoterCB and chicken beta-actin + CMV enhancerAvailable sinceMarch 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJFRC172-10XUAS-loxP>-dSTOP-loxP>-myr::GFP
Plasmid#32145DepositorPublicationInsertloxP> STOP STOP loxP>
ExpressionInsectAvailable sinceSept. 23, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by