We narrowed to 34,848 results for: CaS;

1. DHFR-SpCas9-DHFR

   Plasmid

   #85447

   Purpose

   Expresses SpCas9 fused to DHFR domain on both N- and C-termini in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

   Insert

   DHFR-SpCas9-DHFR
   

   Use

   AAV

   Tags

   Destabilized domain (C-terminus version) of E.col…

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLBH531_MBP-Cas14a1 expression

   Plasmid

   #112500

   Purpose

   Expresses MBP fused to Cas14a1

   Depositor

   Jennifer Doudna

   Article

   Harrington et al Science. 2018 Oct 18. pii: science.aav42

   Insert

   Cas14a1
   

   Use

   CRISPR

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-dCas13-M3M14nes

   Plasmid

   #155367

   Purpose

   Targeted m6A RNA methylation in mammalian cells

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   dCas13-M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   PspCas13b Δ984-1090 H133A

   Promoter

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDmAct5C::Cas9-2A-Neo

   Plasmid

   #176679

   Purpose

   Expression of Drosophila codon optimized spCas9 under Drosophila Act5C promoter

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Insert

   spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
   

   Use

   Crispr

   Tags

   Expression

   Insect

   Mutation

   Drosophila codon optimized

   Promoter

   D. melanogaster Act5C

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiV2-dCas9-VP64

   Plasmid

   #141104

   Purpose

   Expression of gRNA and dCas9-VP64 from the same plasmid for CRISPRa

   Depositor

   Igor Ulitsky

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-RSV-SpCas9

   Plasmid

   #85450

   Purpose

   Express SpCas9 in mammalian cells

   Depositor

   Hetian Lei

   Article

   Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

   Insert

   pRSV
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   pRSV

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. Inducible Caspex expression

   Plasmid

   #97421

   Purpose

   For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics

   Depositor

   Steven Carr, Samuel Myers

   Article

   Myers et al Nat Methods. 2018 May 7. pii: 10.1038/s4

   Inserts

   Caspex
   T2A-Gfp
   

   Use

   CRISPR

   Tags

   3xFLAG and V5

   Expression

   Mammalian

   Mutation

   dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …

   Promoter

   TRE3G

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. PiggyBac dCas9-KRAB

   Plasmid

   #191314

   Purpose

   piggyBac integration and dox-inducible expression of KRAB-dCas9-HA-tagBFP

   Depositor

   Joanna Wysocka

   Article

   Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

   Inserts

   KRAB-dCas9-HA-tagBFP
   rtTA3-IRES-PURO
   

   Use

   CRISPR

   Tags

   HA and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. modRNAc1-VP64dCas9VP64-2A-MPH

   Plasmid

   #216388

   Purpose

   this vector can be used to make modRNA expressing VP64dCas9VP64 and MPH

   Depositor

   Xiaojun Lian

   Article

   Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

   Insert

   VP64dCas9VP64-2A-MPH
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. p160Tol2-her4.3:switchCas9

    Plasmid

    #227680

    Purpose

    Expression of cre inducible and her4.3 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs for SABER-seq in zebrafish

    Depositor

    Bushra Raj

    Article

    Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

    Inserts

    loxP-dsRed-loxP-Cas9-t2A-GFP
    4xU6:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6 and her4.3

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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