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Showing: 3081 - 3100 of 5714 results

  3. pJW2171

    Plasmid
    #163095
    Purpose
    CRISPR repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    30aa linker::mNeonGreen (dpi)::AID*::3xFLAG::10aa linker
    Use
    CRISPR
    Tags
    Expression
    Worm
    Mutation
    Silent mutations to remove piRNA sites
    Promoter
    Available since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163095-plasmid-map-sequence-id-320590

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  6. AAVS1-PDL1

    Plasmid
    #159280
    Purpose
    Human AAVS1 targeting vector for knockin of a CAGGS promoter-driven human PDL1/CD274 (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.
    Depositor
    Rudolf Jaenisch
    Article
    Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d
    Insert
    Human PDL1/CD274 (CD274 Human)
    Use
    AAV; S1 knockin donor vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGGS
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    159280-plasmid-map-sequence-id-312917

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  14. pJW2172

    Plasmid
    #163096
    Purpose
    CRISPR repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    30aa linker::mNeonGreen (dpi)::3xFLAG::10aa linker
    Use
    CRISPR
    Tags
    Expression
    Worm
    Mutation
    Silent mutations to remove piRNA sites
    Promoter
    Available since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163096-plasmid-map-sequence-id-320618

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  16. pGLOW39

    Plasmid
    #173068
    Purpose
    mScarlet^SEC^3xMyc vector with ccdB sites for cloning homology arms
    Depositor
    Ryan Doonan
    Article
    Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791
    Insert
    mScarlet-I-C1^SEC^3xMyc
    Use
    CRISPR and Cre/Lox
    Tags
    3xMyc and C. elegans codon-optimized mScarlet
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    173068-plasmid-map-sequence-id-345944

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  17. pCEP4-HLA-c-myc-optiT1R3 ECD

    Plasmid
    #113960
    Purpose
    mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 extracellular domain with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R3 (TAS1R3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    residues 21-563
    Promoter
    cmv
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113960-plasmid-map-sequence-id-215290

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  18. pDNR-SpCas9-Gem

    Plasmid
    #80424
    Purpose
    Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining
    Depositor
    Melissa Little, Sara Howden
    Article
    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-
    Insert
    SpCas9-Gem
    Use
    CRISPR
    Tags
    Geminin
    Expression
    Mutation
    codon optimized for human
    Promoter
    T7
    Available since
    Aug. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    80424-plasmid-map-sequence-id-151441

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  19. pSUMO-β′-COPI-WD40

    Plasmid
    #180753
    Purpose
    Bacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40 (residues 1-301).
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1
    Insert
    β′-COPI-WD40 (SEC27 Budding Yeast)
    Use
    Tags
    N-terminal strep tag, His-tag, and SUMO tag
    Expression
    Bacterial
    Mutation
    Encodes residues 1-301; deleted 302-889; mutated …
    Promoter
    T7
    Available since
    March 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    180753-plasmid-map-sequence-id-356139

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Showing: 3081 - 3100 of 5714 results