We narrowed to 15,660 results for: genome
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Plasmid#128235PurposeDonor vector for 3' FLAG tag of human ZBTB1DepositorInsertZBTB1 Homology arms
UseCRISPRTags3XFLAG-P2A-NeoRAvailable SinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSTARR-seq_fly-hsp70
Plasmid#71500PurposeflySTARR-seq screening vector with hsp70 core promoterDepositorTypeEmpty backboneUseFlystarr-seq screening vectorTagssgGFP (SuperGlo, Qbiogene, Inc)ExpressionInsectAvailable SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET8
Plasmid#107439PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-luc2
Plasmid#138355PurposeGateway compatible middle entry clone contains Firefly Luciferase2 without minimal promoterDepositorInsertluciferase
ExpressionMammalianAvailable SinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDB4281
Plasmid#98700PurposeA Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX systemDepositorInsertCas9
UseCRISPRExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB4283
Plasmid#98702PurposeA plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX systemDepositorInsertgRNA PCR template
UseCRISPRExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330A-1x7
Plasmid#58771PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKDR-CBh
Plasmid#183557PurposeOne in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (non-attenuated CBh promoter)DepositorInsertUnattenuated
ExpressionMammalianPromoterCBhAvailable SinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pREPK219H
Plasmid#51492PurposepREP with a mutation in the ATPase domain (K219H). This is predicted to result in loss of geminivirus replication.DepositorInsertRep/RepA
UsePlant expression t-dnaMutationchanged lysine 219 to histidinePromoter2x35SAvailable SinceMay 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
BSP605
Plasmid#122250Purposeexpression vector for mCerulean under EFT3 promoterDepositorInsertmCerulean
ExpressionWormPromoterEFT3Available SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR1056
Plasmid#71824PurposeExpress Streptococcus pyogenes dCas9 (D10A, H840A) carrying two C-terminal SV40 NLSDepositorInsertT7pr_6xHis-MBP-TEV-SPydCas9 (D10A,H840A)-2xNLS
UseCRISPRAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX458_CEBPB_1
Plasmid#64036PurposeExpresses gRNA against human CEBPB along with Cas9 with 2A GFPDepositorInsertgRNA
UseCRISPRPromoterU6Available SinceJuly 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMiniT:SEP-beta2 TKIT donor
Plasmid#169436PurposeDonor DNA for TKIT knockin of SEP to Gabrb2 in mouseDepositorInsertSuper ecliptic pHluorin (SEP)
UseCRISPRExpressionMammalianAvailable SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYC1876
Plasmid#158722PurposeHelper plasmid pYC1640 for C-terminal tagging with degradation tag ssrADepositorInsertssrA
UseCRISPRExpressionBacterialAvailable SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
PX458_CEBPB_2
Plasmid#64047PurposeExpresses gRNA against human CEBPB along with Cas9 with 2A GFPDepositorInsertgRNA
UseCRISPRPromoterU6Available SinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSN444
Plasmid#108331PurposeExpresses Sat2 for Nourseothricin resistance selection of C. elegansDepositorInsertStreptothricin acetyl transferase 2
ExpressionWormPromoterrps-27 promoterAvailable SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDU1
Plasmid#169817PurposepSpCas9(BB)-2A-tCD19 (pDU1)DepositorInserttCD19
Tagsfusion proteinExpressionMammalianPromoterCbhAvailable SinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cip-pAct1-Mnase-SAT1
Plasmid#162557PurposeFor the C. auris MNase control strain, the URA3 auxotrophy marker of the CIp-pACT1-3xFLAG-MNase-SV40-CYC was replaced by SAT1DepositorInsert3FLAG-Mnase-SV40
ExpressionYeastPromoterActineAvailable SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ148
Plasmid#69300PurposeGolden Gate recipient and Gateway entry vector; assembly of 8 gRNAsDepositorTypeEmpty backboneUseCRISPRAvailable SinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBAD-6xHis-BglII
Plasmid#196654PurposeDerived from pGLO (BioRad) by the insertion of a 6xHis Tag and a BglII siteDepositorTypeEmpty backboneTags6xHis tagExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only