We narrowed to 7,519 results for: Lif

1. pSCL.94

   Plasmid

   #184985

   Purpose

   Express -Eco1 RT/Cas9 fusion, using linker2

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1RT-fusion_linker2-Cas9
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCL.006

   Plasmid

   #184970

   Purpose

   Express -Eco1 RT in yeast

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Integrating inducible cassette: Eco1 RT
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSCL.005

   Plasmid

   #184971

   Purpose

   Express Cas9 in yeast

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Integrating inducible cassette: Cas9
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   Promoter

   Gal1-10

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSLS.543

   Plasmid

   #184960

   Purpose

   Test effect of a1/a2 length on editing murF using retron recombineering

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 12
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   murF donor G1359A

   Promoter

   T7/lac

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSCL.71

   Plasmid

   #184983

   Purpose

   Express -Eco1 RT/Cas9 fusion, using linker1

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1RT-fusion_linker1-Cas9
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSLS.545

   Plasmid

   #184962

   Purpose

   Test effect of a1/a2 length on editing priB using retron recombineering

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 12
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   priB donor G1069A

   Promoter

   T7/lac

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. KBB106

   Plasmid

   #185065

   Purpose

   Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion including C-terminal half of Nup1 C-terminal FG domain

   Depositor

   Ken Belanger

   Article

   Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

   Insert

   NUP1
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Nup1 C-terminal region, truncated so C-terminal h…

   Promoter

   Available since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMC-9-35S-11

   Plasmid

   #176881

   Purpose

   MoClo Level 0 plasmid, contains 35S

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Insert

   35S terminator
   

   Use

   Tags

   Expression

   Plant

   Mutation

   WT

   Promoter

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLNHA-C1-HsNot8_J

   Plasmid

   #146699

   Purpose

   Mammalian Expression of HsNot8

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

   Insert

   HsNot8 (CNOT8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX458_sgRNA_Ago1_5

    Plasmid

    #172470

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago1

    Depositor

    Constance Ciaudo

    Article

    Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

    Insert

    sgAgo1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX458_sgRNA_Ago1_6

    Plasmid

    #172471

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago1.

    Depositor

    Constance Ciaudo

    Article

    Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

    Insert

    sgAgo1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2+ gcsf

    Plasmid

    #132947

    Purpose

    Expresses zebrafish granulocyte colony stimulating factor (csf3a)

    Depositor

    Jason Berman

    Article

    Rajan et al Leukemia. 2021 Sep 30. pii: 10.1038/s413

    Insert

    gcsf (csf3a Zebrafish)
    

    Use

    Mrna synthesis

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. C453-E18: attB4-polyhedrinp>-attB5

    Plasmid

    #162977

    Purpose

    Gateway attB4/attB5 entry clone for baculovirus expression using the AcMNPV polyhedrin promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    AcMNPV polyhedrin promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pZS160

    Plasmid

    #134238

    Purpose

    Lentiviral plasmid expressing sgRNA against HJURP

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

    Insert

    Human HJURP sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKM328

    Plasmid

    #134243

    Purpose

    Mammalian expression construct expressing sgRNA against PLK1

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

    Insert

    gRNA targeting PLK1 3'
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMET17p-CCG 316

    Plasmid

    #131166

    Purpose

    For ScMET17 promoter regulated expression of mCherry-Cub-R-GFP (CCG) fused bait proteins, centromeric ARS plasmid, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutant

    Depositor

    Nils Johnsson

    Article

    Dunkler et al Methods Mol Biol. 2015;1346:151-68. doi:

    Type

    Empty backbone

    Use

    Tags

    CCG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWPI-TFDP2

    Plasmid

    #114300

    Purpose

    Constitutive expression of the TFDP2 protein

    Depositor

    Patrick Salmon

    Article

    Rady et al Cell Cycle. 2013 Aug 15;12(16):2691-702.

    Insert

    TFDP2 (TFDP2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV(shRNA)-Puro-6 CCEPR Vb2

    Plasmid

    #108278

    Purpose

    shRNA for CCEPR lncRNA

    Depositor

    Karl Munger

    Article

    Sharma et al Virology. 2018 Feb 7;518:8-13. doi: 10.1

    Insert

    CCEPR (CCEPR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMAL-SsoPCNA3

    Plasmid

    #107069

    Purpose

    Expresses S. solfataricus PCNA3 fused to MBP in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

    Insert

    S. solfataricus PCNA3
    

    Use

    Tags

    6xHis-thrombin cleavage site and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNCMV HPV8 E6 R138S

    Plasmid

    #87659

    Purpose

    Flag/HA tagged HPV8 E6 R138S

    Depositor

    Karl Munger

    Article

    Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

    Insert

    HPV 8E6
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    R138S

    Promoter

    CMV

    Available since

    June 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pNCMV HPV8 E6 K64A

    Plasmid

    #87656

    Purpose

    Flag/HA tagged HPV8 E6 K64A

    Depositor

    Karl Munger

    Article

    Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

    Insert

    HPV 8E6
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    K64A

    Promoter

    CMV

    Available since

    March 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pNCMV HPV8 E6 K136N

    Plasmid

    #87657

    Purpose

    Flag/HA tagged HPV8 E6 K136N

    Depositor

    Karl Munger

    Article

    Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

    Insert

    HPV 8E6
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    K136N

    Promoter

    CMV

    Available since

    March 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pNCMV HPV8 E6 K142A

    Plasmid

    #87660

    Purpose

    Flag/HA tagged HPV8 E6 K142A

    Depositor

    Karl Munger

    Article

    Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

    Insert

    HPV 8E6
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    K142A

    Promoter

    CMV

    Available since

    March 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pNCMV HPV8 E6 L59D

    Plasmid

    #87655

    Purpose

    Flag/HA tagged HPV8 E6 L59D

    Depositor

    Karl Munger

    Article

    Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

    Insert

    HPV 8E6
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    L59D

    Promoter

    CMV

    Available since

    March 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pNCMV MmuPV1 E6 R130A

    Plasmid

    #87654

    Purpose

    Flag/HA tagged MmuPV1 E6 R130A

    Depositor

    Karl Munger

    Article

    Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

    Insert

    MmuPV1 E6
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    R130A

    Promoter

    CMV

    Available since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBW215J105-Amp32C

    Plasmid

    #78650

    Purpose

    pSB3K3 carrying J105-30hrpR-32hrpS-t-hrpL-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

    Insert

    J105-30hrpR-32hrpS-t-hrpL-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    see insert

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

27. pBW212J114-Amp32C

    Plasmid

    #78647

    Purpose

    pSB3K3 carrying J114-30hrpR-32hrpS-t-hrpL-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

    Insert

    J114-30hrpR-32hrpS-t-hrpL-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    see insert

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

28. pIhh4.5k-Luc

    Plasmid

    #53603

    Purpose

    Reporter construct containing a 4.5 kb promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays

    Depositor

    Xiangli Yang

    Article

    Wang et al Development. 2009 Dec;136(24):4143-53. d

    Insert

    Ihh promoter
    

    Use

    Luciferase; Reporter

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pGL3-PIM2mut2

    Plasmid

    #51850

    Purpose

    promoter region of PIM2 lacking the forkhead binding site #1

    Depositor

    Gerardo Ferbeyre

    Article

    Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

    Insert

    PIM2 (PIM2 Human)
    

    Use

    Luciferase; Reporter vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2014

    Availability

    Academic Institutions and Nonprofits only

30. roGFP_iR_pQE30

    Plasmid

    #48634

    Purpose

    Bacterial expression vector encoding for the 6XHis tagged redox probe roGFPiR (roGFP C147, R147b, C204)

    Depositor

    David Ron

    Article

    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

    Insert

    roGFP1
    

    Use

    Tags

    HisTag

    Expression

    Bacterial

    Mutation

    insertion R147B

    Promoter

    Available since

    Oct. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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