We narrowed to 15,050 results for: pET

1. PIA900

   Plasmid

   #104401

   Purpose

   Polycistronic vector for expression of E. coli core RNAP

   Depositor

   Irina Artsimovitch

   Article

   Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

   Insert

   rpoA, B, C, and Z
   

   Use

   E. coli expression vector

   Tags

   encodes a C-terminally His-tagged rpoC which can …

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. K560GFP

   Plasmid

   #129761

   Purpose

   Expresses Drosophila Kinesin-1 truncated after coil 1 at 560 and tagged with GFP for single-molecule motor assays

   Depositor

   William Hancock

   Insert

   KHC (Khc Fly)
   

   Tags

   GFP and His6

   Expression

   Bacterial

   Mutation

   Truncation at 559

   Available Since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCL1333

   Plasmid

   #217966

   Purpose

   Bacterial expression of RAN with an N-terminal 6His tag and a TEV cleavage site.

   Depositor

   Andrew Bowman

   Article

   Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

   Insert

   RAN (RAN Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. p-miniTurbo-HA

   Plasmid

   #209637

   Purpose

   Expression miniTurbo-HA in mammalian cells

   Depositor

   Xiang Zhou

   Article

   Lu et al Nucleic Acids Res. 2024 Mar 7:gkae126. d

   Insert

   miniTurbo
   

   Expression

   Mammalian

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. 6xHis-MBP-huBoCas12a

   Plasmid

   #174673

   Purpose

   Expresses 6xHis-MBP tagged BoCas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huBoCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTSA-C

   Plasmid

   #17329

   Depositor

   Takeshi Sano

   Article

   Reznik et al Bioconjug Chem. . 12(6):1000-4.

   Insert

   streptavidin
   

   Expression

   Bacterial

   Mutation

   Truncated to include only amino acids 16-133 of m…

   Available Since

   Feb. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

7. 6xHis-MBP-huPb2Cas12a

   Plasmid

   #174686

   Purpose

   Expresses 6xHis-MBP tagged Pb2Cas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huPb2Cas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCri-1b

   Plasmid

   #61327

   Purpose

   Protein expression.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

   Insert

   Green fluorescent protein
   

   Tags

   His-tag and MBP-tag

   Expression

   Bacterial

   Available Since

   Jan. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pBD-0001

   Plasmid

   #12373

   Depositor

   Axel Brunger

   Article

   DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

   Insert

   VCP (Vcp Mouse)
   

   Tags

   His6

   Expression

   Bacterial

   Available Since

   Aug. 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKEW397_AcrIIA15

    Plasmid

    #138285

    Purpose

    Expression of AcrIIA15 protein

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    AcrIIA15
    

    Use

    CRISPR

    Tags

    MBP

    Expression

    Bacterial

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKEW394_AcrIIA14

    Plasmid

    #138283

    Purpose

    Expression of AcrIIA14 protein

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    AcrIIA14
    

    Use

    CRISPR

    Tags

    SFGFP

    Expression

    Bacterial

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. KRigor980

    Plasmid

    #129763

    Purpose

    Expresses full-length Drosophila Kinesin-1 with rigor mutation (R210A) for adhering microtubules to surfaces

    Depositor

    William Hancock

    Insert

    KHC (Khc Fly)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    R210A

    Available Since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. K560_AviC_coBirA

    Plasmid

    #129765

    Purpose

    Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assays

    Depositor

    William Hancock

    Insert

    KHC (Khc Fly)
    

    Tags

    Avi-tag GLNDIFEAQKIEWH and His6

    Expression

    Bacterial

    Mutation

    Truncation at 559

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAM282

    Plasmid

    #226121

    Purpose

    Expression of cdc48

    Depositor

    Andreas Martin

    Article

    Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

    Insert

    His6-3C-Cdc48 (CDC48 Budding Yeast)
    

    Expression

    Bacterial

    Available Since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDJSVT91

    Plasmid

    #109305

    Purpose

    Express periplasmic, outer membrane, or secreted proteins in E. coli - SufI signal (TAT) and His6 tag

    Depositor

    Daniel Slade

    Article

    Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

    Type

    Empty backbone

    Tags

    SufI 1-30, 6His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBD-0016

    Plasmid

    #12345

    Depositor

    Axel Brunger

    Article

    DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

    Insert

    VCP R313K (Vcp Mouse)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    R313K, I206V*(see below)

    Available Since

    Aug. 24, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pUG-124

    Plasmid

    #104635

    Purpose

    Level 2 construct

    Depositor

    Marco Trujillo

    Article

    Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

    Insert

    HIS-Ubq_UBA1_HA-UBC8_GST-AvrPtoB_EL4
    

    Tags

    HIS-Ubq, HA-E2, GST-E3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCri-13a

    Plasmid

    #61321

    Purpose

    Protein expression.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

    Insert

    Green fluorescent protein
    

    Tags

    His-tag and MISTIC-tag

    Expression

    Bacterial

    Available Since

    Jan. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDJSVT89

    Plasmid

    #109303

    Purpose

    Express periplasmic, outer membrane, or secreted proteins in E. coli - TamA signal and His6 tag

    Depositor

    Daniel Slade

    Article

    Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

    Type

    Empty backbone

    Tags

    TamA 1-27, 6His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKEW215_AcrIIA13

    Plasmid

    #138281

    Purpose

    Expression of AcrIIA13 protein

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    AcrIIA13
    

    Use

    CRISPR

    Tags

    MBP

    Expression

    Bacterial

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. K560_AviN_coBirA

    Plasmid

    #129766

    Purpose

    Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated on the head for single-molecule assays

    Depositor

    William Hancock

    Insert

    KHC (Khc Fly)
    

    Tags

    Avi-tag GLNDIFEAQKIEWH and His6

    Expression

    Bacterial

    Mutation

    Truncation at 559

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p2CT-Cdi

    Plasmid

    #83044

    Purpose

    Expresses Corynebacterium diphtheriae Cas9 (CdiCas9)

    Depositor

    Jennifer Doudna

    Article

    Ma et al Mol Cell. 2015 Nov 5;60(3):398-407. doi:

    Insert

    CdiCas9
    

    Tags

    His-MBP

    Expression

    Bacterial

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

23. KaiB-FLAG

    Plasmid

    #41885

    Depositor

    Michael Rust

    Article

    Phong et al Proc Natl Acad Sci U S A. 2013 Jan 15;11

    Insert

    Kai B
    

    Tags

    FLAG

    Expression

    Bacterial

    Promoter

    t7 promoter

    Available Since

    March 19, 2013

    Availability

    Academic Institutions and Nonprofits only

24. 6xHis-MBP-huLb5Cas12a

    Plasmid

    #174684

    Purpose

    Expresses 6xHis-MBP tagged Lb5Cas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huLb5Cas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCri-8a

    Plasmid

    #61317

    Purpose

    Protein expression.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

    Insert

    Green fluorescent protein
    

    Tags

    His-tag

    Expression

    Bacterial

    Available Since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

26. pCri-4b

    Plasmid

    #61328

    Purpose

    Protein expression.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

    Insert

    Green fluorescent protein
    

    Tags

    His-tag and TRX-tag

    Expression

    Bacterial

    Available Since

    Jan. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

27. 6xHis-MBP-huHkCas12a

    Plasmid

    #174683

    Purpose

    Expresses 6xHis-MBP tagged HkCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huHkCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCri-11b

    Plasmid

    #61333

    Purpose

    Protein expression.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

    Insert

    Green fluorescent protein
    

    Tags

    His-tag and SUMO-tag

    Expression

    Bacterial

    Available Since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

29. K406

    Plasmid

    #129764

    Purpose

    Expresses Drosophila Kinesin-1 truncated after neck-coil at 406

    Depositor

    William Hancock

    Insert

    KHC (Khc Fly)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Truncation at 406

    Available Since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Human Mevalonate Kinase (MK)

    Plasmid

    #52429

    Purpose

    bacterial expression of human Mevalonate Kinase (MK)

    Depositor

    Henry Miziorko

    Article

    Potter et al J Biol Chem. 1997 Oct 10;272(41):25449-5

    Insert

    Human Mevalonate Kinase (MVK Human)
    

    Expression

    Bacterial

    Mutation

    see comments

    Promoter

    T7

    Available Since

    April 10, 2014

    Availability

    Academic Institutions and Nonprofits only