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pCMV-Tag2B Flag BimS

Plasmid

#23283

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

pCMV Tag2b Bim S (Bcl2l11 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only
pAGM4673

Plasmid

#48014

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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Klf2-t2A-mCherry

Plasmid

#127539

Purpose

Expresses mouse Klf2 and mCherry via t2A linker.

Depositor

Jennifer Mitchell

Article

Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

Insert

Klf2 (Klf2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

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GST-AR-624-919

Plasmid

#104200

Purpose

Expresses human AR-624-929 ligand binding domain in pGEX-5X-1 as GST fusion protein

Depositor

Elizabeth Wilson

Article

He et al Mol Cell Biol. 2003 Mar;23(6):2135-50.

Insert

human androgen receptor aa 624-919 (AR Human)

Tags

GST

Expression

Bacterial

Available Since

Dec. 6, 2017

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-Vinculin-23

Plasmid

#55334

Purpose

Localization: Focal Adhesions, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Vinculin (VCL Human)

Tags

mTagBFP2

Expression

Mammalian

Mutation

V234L in Vinculin.

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
peCALWY-3

Plasmid

#22235

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY-3 Zn(II) sensor (ATOX1 Human, GFP's)

Expression

Mammalian

Mutation

Similar to eCALWY-1, but with a linkerlength of 3…

Available Since

Nov. 20, 2009

Availability

Academic Institutions and Nonprofits only
pPSU100

Plasmid

#173835

Purpose

Expresses 100 kD Penn State ladder protein

Depositor

Song Tan

Article

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPABPACIL1bQRS

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2 sgNRF2-3

Plasmid

#186838

Purpose

knock out NRF2 in mammalian cells

Depositor

Boyi Gan

Article

Koppula et al Nat Commun. 2022 Apr 22;13(1):2206. doi:

Insert

Nrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)

Use

CRISPR and Lentiviral

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-LC3

Plasmid

#110947

Purpose

Expresses the autophagosome marker LC3 fused to mTurquoise2

Depositor

Pablo Rivera-Fuentes

Article

Nguyen et al Org Biomol Chem. 2021 Mar 28;19(12):2681

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Tags

mTurquoise2

Expression

Mammalian

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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aCat TL1 (1-907 mTFP1-TSmod-Venus STOP)

Plasmid

#101297

Purpose

FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

Depositor

Alpha Yap

Article

Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

Insert

alpha-Catenin

Expression

Mammalian

Mutation

Contains amino acids 1-906 of alpha-Catenin

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLX317-QKI

Plasmid

#115441

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

QKI (QKI Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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mOPN-pcDNA

Plasmid

#106447

Purpose

Mammalian expression of murine osteopontin

Depositor

Georg Weber

Article

Zhang et al Mol Cell Biol. 2003 Sep . 23(18):6507-19

Insert

osteopontin (Spp1 Mouse)

Expression

Mammalian

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-Rab27B T23N

Plasmid

#89450

Purpose

Expression of GFP-tagged human Rab27B T23N in mammalian cells

Depositor

Wendy Westbroek

Article

Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

Insert

human Rab27B T23N (RAB27B Human)

Tags

eGFP

Expression

Mammalian

Mutation

T23N

Promoter

CMV

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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CbSBS-Flag/TRRAP(S722F)

Plasmid

#32105

Depositor

Yardena Samuels

Insert

Transformation/transcription domain-associated protein (S722F) (TRRAP Human)

Tags

Flag 1x

Expression

Mammalian

Mutation

Changes serine 722 to Phe

Promoter

CMV

Available Since

Nov. 14, 2011

Availability

Academic Institutions and Nonprofits only
pRDA_886

Plasmid

#201162

Purpose

Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' VP64 and NLS

Depositor

John Doench

Article

Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

Insert

Puromycin resistance

Use

CRISPR and Lentiviral

Promoter

EFS

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.SF-iGluSnFR.S72A

Plasmid

#106200

Purpose

Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.S72A

Use

AAV

Mutation

GltI: S72A

Promoter

CAG

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCORE-UH

Plasmid

#72239

Purpose

Contains the CORE cassette with KlURA3 and hyg markers

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Genet Eng (N Y). 2003;25:189-207.

Inserts

KlURA3
hyg

Expression

Bacterial

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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p(U6a-BsaI-gRNA)

Plasmid

#65955

Purpose

Tribolium U6a promoter with BsaI cloning site to insert guide RNA sequence

Depositor

Michalis Averof

Article

Gilles et al Development. 2015 Jul 9. pii: dev.125054

Type

Empty backbone

Expression

Insect

Promoter

U6

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pRetroNeo

Plasmid

#21740

Depositor

Clare Huxley

Article

Magin-Lachmann et al BMC Biotechnol. 2003 Feb 3. 3(1):2.

Type

Empty backbone

Use

N/a

Available Since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

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MIGS5.1 (FF572)

Plasmid

#35253

Depositor

Detlef Weigel

Article

de Felippes et al Plant J. 2011 Dec 28. doi: 10.1111/j.136

Type

Empty backbone

Expression

Plant

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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