We narrowed to 10,458 results for: T7

1. pMS13

   Plasmid

   #229474

   Purpose

   Expresses RssB with Q254A & N256A mutation

   Depositor

   Alexandra Deaconescu

   Article

   Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

   Insert

   RssB (Q254A, N256A)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Q254A, N256A

   Promoter

   T7

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMS19

   Plasmid

   #229475

   Purpose

   Expresses RssB with E135A, E136A, & E137A mutation

   Depositor

   Alexandra Deaconescu

   Article

   Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

   Insert

   RssB (E135A, E136A, E137A)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E135A, E136A, E137A

   Promoter

   T7

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pVF42

   Plasmid

   #229467

   Purpose

   Expresses RssB with S29C & E229C mutation

   Depositor

   Alexandra Deaconescu

   Article

   Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

   Insert

   RssB (S29C, E229C)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S29C, E229C

   Promoter

   T7

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMS21

   Plasmid

   #229476

   Purpose

   Expresses RssB with E135A, E136A, & E137A mutation with His-tag

   Depositor

   Alexandra Deaconescu

   Article

   Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

   Insert

   RssB (E135A, E136A, E137A)
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   E135A, E136A, E137A

   Promoter

   T7

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMS26

   Plasmid

   #229471

   Purpose

   Expresses RssB with D104N mutation with His-tag

   Depositor

   Alexandra Deaconescu

   Article

   Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

   Insert

   RssB (D104N)
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   D104N

   Promoter

   T7

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMS23

   Plasmid

   #229470

   Purpose

   Expresses RssB with D104A mutation with His-tag

   Depositor

   Alexandra Deaconescu

   Article

   Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

   Insert

   RssB (D104A)
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   D104A

   Promoter

   T7

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMS18

   Plasmid

   #229462

   Purpose

   Expresses IraD with Q68A mutation with His-tag

   Depositor

   Alexandra Deaconescu

   Article

   Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

   Insert

   IraD (Q68A)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Q68A

   Promoter

   T7

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMS12

   Plasmid

   #229461

   Purpose

   Expresses IraD with W64R mutation with His-tag

   Depositor

   Alexandra Deaconescu

   Article

   Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

   Insert

   IraD (W64R)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   W64R

   Promoter

   T7

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pVF40

   Plasmid

   #229459

   Purpose

   Expresses IraD with R70A mutation with His-tag

   Depositor

   Alexandra Deaconescu

   Article

   Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

   Insert

   IraD (R70A)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   R70A

   Promoter

   T7

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pVF26

    Plasmid

    #229458

    Purpose

    Expresses IraD with Q84A mutation with His-tag

    Depositor

    Alexandra Deaconescu

    Article

    Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

    Insert

    IraD (Q84A)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Q84A

    Promoter

    T7

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pVF25

    Plasmid

    #229457

    Purpose

    Expresses IraD with R67A mutation with His-tag

    Depositor

    Alexandra Deaconescu

    Article

    Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

    Insert

    IraD (R67A)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R67A

    Promoter

    T7

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PET-28a-YTH domain of YTHDC2

    Plasmid

    #228779

    Purpose

    Protein expression plasmid for YTH domain of YTHDC2 in E.Coli.

    Depositor

    Huiqing Zhou

    Article

    Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

    Insert

    YTH domain of YTHDC2 (YTHDC2 Human)
    

    Use

    Tags

    6*His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PET-28a-YTH domain of YTHDC1

    Plasmid

    #228778

    Purpose

    Protein expression plasmid for YTH domain of YTHDC1 in E.Coli.

    Depositor

    Huiqing Zhou

    Article

    Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

    Insert

    YTH domain of YTHDC1 (YTHDC1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CLIPf-KIF5A(1-573)

    Plasmid

    #219683

    Purpose

    Mammalian expression of CLIPf-KIF5A(1-573)

    Depositor

    Alison Twelvetrees

    Article

    Smith et al bioRxiv 2024.12.20.629623

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Tags

    CLIPf

    Expression

    Mammalian

    Mutation

    1-573

    Promoter

    CMV & T7

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. CLIPf-KIF5A-SNAPf-HisTag-FLAG

    Plasmid

    #200789

    Purpose

    Mammalian expression of CLIPf-KIF5A-SNAPf-HisTag-FLAG

    Depositor

    Alison Twelvetrees

    Article

    Smith et al bioRxiv 2024.12.20.629623

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Tags

    CLIPf and SNAPf, HisTag, FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV & T7

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTBY1-T4gp61/41 fusion

    Plasmid

    #228210

    Purpose

    Expression in BL21(DE3) of T4 primase and helicase fusion with 24 amino acid linker

    Depositor

    Stephen Benkovic

    Article

    Manosas et al Nat Chem Biol. 2009 Dec;5(12):904-12. do

    Insert

    61/41
    

    Use

    Tags

    Sce VMA intein-chitin binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SpyCatcher-P-Domain

    Plasmid

    #201193

    Purpose

    Expresses furin P-domain genetically fused to SpyCatcher001 in bacteria

    Depositor

    Vesa Hytönen

    Article

    Lampinen et al Nanoscale Adv. 2024 Oct 9;6(24):6239-52.

    Insert

    SpyCatcher-P-Domain
    

    Use

    Tags

    HisTag and SpyCatcher001

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pACYCDuet-1:ChrA-C66S

    Plasmid

    #225081

    Purpose

    Expresses precursor peptide ChrA-C66S in E.coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

    Insert

    ChrA-C66S
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    C66S

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAM57

    Plasmid

    #227654

    Purpose

    pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, V80A)

    Depositor

    Andreas Martin

    Article

    Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-

    Inserts

    Rpn11 (RPN11 Budding Yeast)
    Rpn8 (RPN8 Budding Yeast)
    

    Use

    Tags

    H6-PreScission and PreScission-Strep

    Expression

    Bacterial

    Mutation

    1-179 and amino acids 2-239, V80A

    Promoter

    T7

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAM54

    Plasmid

    #227653

    Purpose

    pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, L132A)

    Depositor

    Andreas Martin

    Article

    Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-

    Inserts

    Rpn11 (RPN11 Budding Yeast)
    Rpn8 (RPN8 Budding Yeast)
    

    Use

    Tags

    H6-PreScission and PreScission-Strep

    Expression

    Bacterial

    Mutation

    1-179 and amino acids 2-239, L132A

    Promoter

    T7

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAM72

    Plasmid

    #227622

    Purpose

    pETDuet-1 Rpn8(1-179)-precission-Strep, H6-precission-Rpn11(2-239, G77P)

    Depositor

    Andreas Martin

    Article

    Worden et al Mol Cell. 2017 Sep 7;67(5):799-811.e8. d

    Inserts

    Rpn11 (RPN11 Budding Yeast)
    rpn8 (RPN8 Budding Yeast)
    

    Use

    Tags

    His-Prescission

    Expression

    Bacterial

    Mutation

    1-179 and aa 2-239, G77P

    Promoter

    T7

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAM71

    Plasmid

    #227621

    Purpose

    pETDuet-1 H6-precission-Rpn11(24-160-GGSGSG-192-221) , Rpn8(1-179)

    Depositor

    Andreas Martin

    Article

    Worden et al Mol Cell. 2017 Sep 7;67(5):799-811.e8. d

    Inserts

    Rpn11 (RPN11 Budding Yeast)
    rpn8 (RPN8 Budding Yeast)
    

    Use

    Tags

    His-Prescission

    Expression

    Bacterial

    Mutation

    1-179 and amino acids 24-160-GGSGSG-192-221

    Promoter

    T7

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCDF-His_TEV_MsbA

    Plasmid

    #191476

    Purpose

    Expresses MsbA with a TEV protease cleavable N terminal His Tag

    Depositor

    Arthur Laganowsky

    Article

    Lyu et al Nat Commun. 2022 Nov 26;13(1):7291. doi:

    Insert

    MsbA
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJL CusS NarX Chimera

    Plasmid

    #225652

    Purpose

    Expresses a NarX-based chimera capable of sensing copper (II). For use with NarL-YdfI and reporter plasmids from this study.

    Depositor

    Neha Kamat

    Article

    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

    Insert

    CusS-NarX
    

    Use

    Cell free expression

    Tags

    Myc

    Expression

    Bacterial

    Mutation

    Transmembrane and sensing domain replaced to dete…

    Promoter

    T7

    Available since

    Dec. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28a-hfYFP-TEV-SAV

    Plasmid

    #191197

    Purpose

    Fusion of hfYFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    hfYFP & Streptavidin fusion
    

    Use

    Tags

    6xHis and Streptavidin core domain, amino acids 1…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28a-LSSmGFP-TEV-SAV

    Plasmid

    #191200

    Purpose

    Fusion of LSSmGFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    LSSmGFP & Streptavidin fusion
    

    Use

    Tags

    6xHis and Streptavidin core domain, amino acids 1…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJL NrsS NarX Chimera

    Plasmid

    #225653

    Purpose

    Expresses a NarX-based chimera capable of sensing nickel (II). For use with NarL-YdfI and reporter plasmids from this study.

    Depositor

    Neha Kamat

    Article

    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

    Insert

    NrsS-NarX
    

    Use

    Cell free expression

    Tags

    Myc

    Expression

    Bacterial

    Mutation

    Transmembrane and sensing domain replaced to dete…

    Promoter

    T7

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJL RssA NarX Chimera

    Plasmid

    #225654

    Purpose

    Expresses a NarX-based chimera capable of sensing iron (III). For use with NarL-YdfI and reporter plasmids from this study.

    Depositor

    Neha Kamat

    Article

    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

    Insert

    RssA-NarX
    

    Use

    Cell free expression

    Tags

    Myc

    Expression

    Bacterial

    Mutation

    Transmembrane and sensing domain replaced to dete…

    Promoter

    T7

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJL NarX Myc

    Plasmid

    #225651

    Purpose

    Expresses WT NarX capable of sensing nitrate. For use with NarL-YdfI and reporter plasmids from this study.

    Depositor

    Neha Kamat

    Article

    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

    Insert

    NarX (narX E. coli)
    

    Use

    Cell free expression

    Tags

    Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJL VanS NarX Chimera

    Plasmid

    #225655

    Purpose

    Expresses a NarX-based chimera capable of sensing vancomycin. For use with NarL-YdfI and reporter plasmids from this study.

    Depositor

    Neha Kamat

    Article

    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

    Insert

    VanS-NarX
    

    Use

    Cell free expression

    Tags

    Myc

    Expression

    Bacterial

    Mutation

    Transmembrane and sensing domain replaced to dete…

    Promoter

    T7

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pACYCDuet-1:ChrA-C63S

    Plasmid

    #225080

    Purpose

    Expresses precursor peptide ChrA-C63S in E.coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

    Insert

    ChrA-C63S
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    C63S

    Promoter

    T7

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pACYC-yhaM

    Plasmid

    #207650

    Purpose

    Overexpression of native L-cysteine desulfidase in E. coli MG1655

    Depositor

    Benjamin Woolston

    Article

    Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

    Insert

    yhaM
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pACYC-decR

    Plasmid

    #207653

    Purpose

    Overexpression of L-cysteine desulfidase transcriptional regulator in E. coli MG1655

    Depositor

    Benjamin Woolston

    Article

    Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

    Insert

    decR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET28a:ChrH

    Plasmid

    #225084

    Purpose

    Expresses multinuclear non-heme iron dependent oxidative enzyme ChrH in E.coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

    Insert

    ChrH
    

    Use

    Tags

    His6-Thrombin

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET28a:ChrI

    Plasmid

    #225085

    Purpose

    Expresses anthrone-oxygenase like enzyme ChrI in E.coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

    Insert

    ChrI
    

    Use

    Tags

    His6 and His6-Thrombin

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET-NIR-FbLAG30

    Plasmid

    #220748

    Purpose

    NIR-FbLAG30 expression in bacteria.

    Depositor

    Vladislav Verkhusha

    Article

    Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

    Insert

    NIR-FbLAG30
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Bacterial

    Mutation

    For bacterial cytoplasmic expression, the pelB si…

    Promoter

    T7

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. A99-ELP

    Plasmid

    #170760

    Purpose

    A99 laminin α1 peptide fused to I30-ELP (VPGIG)30

    Depositor

    Andrew MacKay

    Article

    Truong et al Biomacromolecules. 2024 Jul 8;25(7):4001

    Insert

    A99-ELP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. AG73-ELP

    Plasmid

    #170762

    Purpose

    AG73 laminin α1 peptide fused to I30-ELP (VPGIG)30

    Depositor

    Andrew MacKay

    Article

    Truong et al Biomacromolecules. 2024 Jul 8;25(7):4001

    Insert

    AG73-ELP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAP007

    Plasmid

    #206809

    Purpose

    pET-6xHis-MBP-TEV-BabTIR-APAZ/BabAgo - Heterologous BabSPARTA expression

    Depositor

    Daan Swarts

    Article

    Finocchio et al Nucleic Acids Res. 2024 Feb 28;52(4):201

    Insert

    BabSPARTA operon (6xHis-MBP-BabTIR-APAZ and BabAgo)
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDEST17-ScChd1[R126A/R130A]

    Plasmid

    #221197

    Purpose

    ScChd1-aa118-1274 chromatin remodeler with R126A and R130A mutations

    Depositor

    Gregory Bowman

    Article

    Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

    Insert

    ScChd1 (aa118-1274)
    

    Use

    Tags

    6xHis-tag (Precission protease cleavable)

    Expression

    Bacterial

    Mutation

    R126A, R130A

    Promoter

    T7

    Available since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge