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pUdOm2.4

Plasmid

#210281

Purpose

Empty backbone with ampicillin resistance for low expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pUdOm6.1

Plasmid

#210286

Purpose

Empty backbone with trimethoprim resistance for high expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pUdOm1.0

Plasmid

#210298

Purpose

Empty backbone with ampicillin resistance for high expression in mammalian cells with an added 5'UTR

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pUdOm1.2

Plasmid

#210274

Purpose

Empty backbone with ampicillin resistance for high expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUdOm1.3

Plasmid

#210275

Purpose

Empty backbone with ampicillin resistance for intermediate expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUdOm1.4

Plasmid

#210276

Purpose

Empty backbone with ampicillin resistance for low expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pUdOm1.5

Plasmid

#210277

Purpose

Empty backbone with ampicillin resistance for low expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUdOm2.1

Plasmid

#210278

Purpose

Empty backbone with ampicillin resistance for high expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pUdO1c

Plasmid

#210230

Purpose

high-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pUdO4t

Plasmid

#210249

Purpose

low-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pUdO4k

Plasmid

#210237

Purpose

low-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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p3X-UASTattB

Plasmid

#189859

Purpose

Fly cloning and transformation

Depositor

Jung Hwan Kim

Article

Singh et al MicroPubl Biol. 2022 Aug 12;2022. doi: 1

Type

Empty backbone

Expression

Insect

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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p2X-UASTattB

Plasmid

#189860

Purpose

Fly cloning and transformation

Depositor

Jung Hwan Kim

Article

Singh et al MicroPubl Biol. 2022 Aug 12;2022. doi: 1

Type

Empty backbone

Expression

Insect

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pUdO4z

Plasmid

#210253

Purpose

low-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUdO2z

Plasmid

#210251

Purpose

medium-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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mRuby2-PTP1b

Plasmid

#234748

Purpose

Mammalian expression of endoplasmic reticulum marker mRuby2-PTPb

Depositor

Nevin Lambert

Article

Jang et al Elife. 2024 Nov 8;13:RP97033. doi: 10.75

Insert

mRuby2-PTP1b

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pHaloTag-PS(RA)

Plasmid

#86627

Purpose

Expresses Akt pseudosubstrate (P-3 RA) mutant tagged with a HaloTag

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

HaloTag-PS(RA)

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn1_HyPer7_cyto

Plasmid

#238967

Purpose

Mammalian neurons HyPer7 expression

Depositor

Dmitry Bilan

Article

Kelmanson et al Redox Biol. 2021 Nov 3;48:102178. doi: 1

Insert

HyPer7

Use

AAV

Expression

Mammalian

Promoter

hSyn1

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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RB793

Plasmid

#199121

Purpose

This plasmid contains a barcoding cassette which gets integrated at Neut5 locus in Candida albicans genome

Depositor

Richard Bennett

Article

Kakade et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

Insert

100 bp barcoding cassette

Expression

Yeast

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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RB794

Plasmid

#199122

Purpose

This plasmid contains a barcoding cassette which gets integrated at Neut5 locus in Candida albicans genome

Depositor

Richard Bennett

Article

Kakade et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

Insert

100 bp barcoding cassette

Expression

Yeast

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pACYC_HA-ZmSAE1

Plasmid

#91942

Purpose

Bacterial expression of maize SAE1

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Insert

HA-ZmSAE1

Tags

HA

Expression

Bacterial

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-TMEM30A-V5

Plasmid

#225510

Purpose

TMEM30A expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

Insert

TMEM30A (TMEM30A Human)

Tags

V5

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLX302_CdTag-HA_EmptyVector

Plasmid

#221465

Purpose

Gateway backbone for C-terminal dTag cloning

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Type

Empty backbone

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn1_SypHer3s_mito

Plasmid

#238972

Purpose

Mammalian neurons SypHer3s expression targeted to the mitochondrial matrix

Depositor

Dmitry Bilan

Article

Kelmanson et al Redox Biol. 2021 Nov 3;48:102178. doi: 1

Insert

SypHer3s

Use

AAV

Tags

3x Cytochrome C Oxidase Subunit VIII presequense

Expression

Mammalian

Promoter

hSyn1

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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CMV+ plasmid

Plasmid

#169740

Purpose

Unregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels. This plasmid has additional sequences in the UTRs to increase gene expression levels.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

pCMV-tetO2

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pACYC_ZmSAE2-FLAG

Plasmid

#91943

Purpose

Bacterial expression of maize SAE2

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Insert

ZmSAE2-FLAG

Tags

FLAG

Expression

Bacterial

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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His-TEV-U_(+36GFP)-Cre

Plasmid

#71750

Purpose

His-TEV-U+36GFP-Cre for E.Coli expression

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

U_(pos36GFP)-Cre

Tags

6X His and TEV protease

Expression

Bacterial

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry pBSDONR P4r-P2

Plasmid

#121528

Purpose

For making C-terminal mCherry fusions using multisite Gateway cloning

Depositor

Roger Innes

Article

Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.

Insert

mCherry

Expression

Bacterial

Available Since

Aug. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-mCherry-CLCN3

Plasmid

#225514

Purpose

Lentiviral mCherry-CLCN3 expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

Insert

CLCN3 (CLCN3 Human)

Use

Lentiviral

Tags

mCherry

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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GST-RNF126 C231A

Plasmid

#138644

Purpose

Bacterial expression of GST-RNF126 RING mutant (C231A) fusion protein

Depositor

Jane McGlade

Article

Smith et al J Cell Sci. 2013 Mar 15;126(Pt 6):1366-8

Insert

RNF126 (RNF126 Mouse)

Tags

GST

Expression

Bacterial

Mutation

C231A

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-UVRAG S498D

Plasmid

#86745

Purpose

N-terminal mCherry-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

Insert

human UVRAG (UVRAG Human)

Tags

mCherry

Expression

Mammalian

Mutation

S498D

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-UVRAG S498D

Plasmid

#86742

Purpose

N-terminal EGFP-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

Insert

human UVRAG (UVRAG Human)

Tags

EGFP

Expression

Mammalian

Mutation

S498D

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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myc-UVRAG S498D

Plasmid

#86739

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

Insert

human UVRAG (UVRAG Human)

Tags

myc

Expression

Mammalian

Mutation

S498D

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
palphaGGFPR

Plasmid

#64377

Purpose

Alpha-gliadin gene promoter driving GFP expression

Depositor

Claudia Vickers

Article

Vickers et al Plant Mol Biol. 2006 Sep;62(1-2):195-214

Insert

alpha-Gliadin promoter

Tags

GFP

Expression

Plant

Promoter

alpha-Gliadin

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only
pOAGFPR

Plasmid

#64397

Purpose

Oat avenin promoter driving GFP expression

Depositor

Claudia Vickers

Article

Vickers et al Plant Mol Biol. 2006 Sep;62(1-2):195-214

Insert

Oat avenin promoter

Tags

GFP

Expression

Plant

Promoter

Oat avenin

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA -CLCN3-3xFlag

Plasmid

#225506

Purpose

CLCN3 expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

Insert

CLCN3 (CLCN3 Human)

Tags

3xFlag

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO1031

Plasmid

#235706

Purpose

Expression of FL human Beclin 1 and FL human UVRAG in mammalian cells

Depositor

Roger Williams

Article

Ohashi et al Elife. 2020 Jun 30;9:e58281. doi: 10.755

Inserts

Beclin 1 (BECN1 Human)
UVRAG (UVRAG Human)

Expression

Mammalian

Promoter

CAG and CMV

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1125

Plasmid

#235742

Purpose

Protein expression of GST-tagged PX domain (Cys at 2) in bacteria

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

p40 PX domain (NCF4 Human)

Tags

GST-TEV

Expression

Bacterial

Mutation

aa 2-149 with Cys at 2

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_NKX2-5

Plasmid

#221479

Purpose

Gateway cloning of NKX2-5 overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-5 (NKX2-5 Human)

Use

Gateway Cloning

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
CSF1R_Halo_C_allele

Plasmid

#178139

Purpose

Donor vector for endogenous tagging of human CSF1R at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (CSF1R Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CL20_mEGFP_SSBP2_CSF1R

Plasmid

#205928

Purpose

Express mEGFP-tagged fusion protein, SSBP2_CSF1R from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

SSBP2_CSF1R (SSBP2, CSF1R Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSF_6His-ZmSUMO1a-GG

Plasmid

#91929

Purpose

Bacterial expression of maize SUMO1a

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Insert

6His-ZmSUMO1a-GG

Tags

6His

Expression

Bacterial

Mutation

Truncation of ZmSUMO1a after the diGlycine motif

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CMVenh synapsin-intron-eGFP

Plasmid

#247941

Purpose

AAV transfer plasmid encoding enhanced Green Fluoresence Protein control of the CMVie enhanced synapsin1 promoter

Depositor

Veerle Baekelandt

Article

Oliveras-Salva et al Mol Neurodegener. 2013 Nov 25;8:44. doi:

Insert

eGFP

Use

AAV

Promoter

CMVenh synapsin

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_NKX2-1

Plasmid

#221472

Purpose

Gateway cloning of NKX2-1 short isoform overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway Cloning

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-11-LECT2-V40

Plasmid

#183031

Purpose

Expression of LECT2 V40 variant protein from T7 promoter

Depositor

Gareth Morgan

Article

Belonogov et al bioRxiv 2022.03.01.482540

Insert

LECT2 (LECT2 Human)

Expression

Bacterial

Mutation

V40 variant

Promoter

T7

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
SEPLuc

Plasmid

#141102

Purpose

bioluminescent reporter consisting of a membrane-bound SEP and Nanoluc fusion; for monitoring extracellular tumor acidosis

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

SEP-Nanoluc

Use

Lentiviral

Promoter

EF1α

Available Since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Rab11 single guide RNA

Plasmid

#229683

Purpose

guide RNA for Rab11 tagging at the N-terminus in pX459

Depositor

Francesca Bottanelli

Article

Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

Insert

Rab11 guide (RAB11A Human)

Use

CRISPR

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFX-iRFP-Rab5

Plasmid

#174456

Purpose

Express iRFP with early endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

iRFP with early endosome-targeting sequence

Tags

small GTPase (Rab5)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFX-iRFP-Rab7

Plasmid

#174459

Purpose

Express iRFP with late endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

iRFP with late endosome-targeting sequence

Tags

small GTPase (Rab7)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
plenti-UBC-HA-TMEM230

Plasmid

#225516

Purpose

Lentiviral TMEM230 expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

Insert

TMEM230 (TMEM230 Human)

Use

Lentiviral

Tags

3xHA

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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