Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 12,214 results for: LEA

Clear keywords
Showing: 3121 - 3140 of 12214 results

  1. Sniper-Cas9

    Plasmid
    #138559
    Purpose
    Expresses human codon-optimized Sniper-SpCas9 and blasticidin resistance: EFS promoter-Sniper-Cas9-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    Sniper-Cas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    F539S, M763I, K890N
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    138559-plasmid-map-sequence-id-298901

    Enlarge

  2. pCAGG-NFIC2

    Plasmid
    #112701
    Purpose
    Expression of NFIC2 protein and GFP
    Depositor
    Richard Gronostajski
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nuclear Factor I C2 (Nfic Mouse)
    Tags
    GFP and HA
    Expression
    Mammalian
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112701-plasmid-map-sequence-id-212389

    Enlarge

  7. SRPK1 (SRPK1A-c100)

    Plasmid
    #98246
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SRPK1A (SRPK1 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Sept. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    98246-plasmid-map-sequence-id-193287

    Enlarge

  9. pLV-EF1A-CD19.8H.8TM.28.BB.3z

    Plasmid
    #200672
    Purpose
    Modular CD8hinge-CD8transmembrane-CD28-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production
    Depositor
    Michele Bernasconi
    Publication
    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):
    Insert
    FMC63-CD8HD-CD8TM-CD28-41BB-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)
    Use
    Lentiviral
    Tags
    MycTag
    Expression
    Mammalian
    Promoter
    EF1a-short
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    200672-plasmid-map-sequence-id-391910

    Enlarge

  10. AAVS1_puro_CAG_HexaPro

    Plasmid
    #164077
    Purpose
    Targeted integration of SARS-CoV-2 spike HexaPro variant into the AAVS1 genomic safe harbor locus
    Depositor
    Yannick Doyon
    Publication
    Rivest et al protocols.io 2021
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 S HexaPro (S Severe acute respiratory syndrome coronavirus 2)
    Use
    CRISPR
    Tags
    2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CAG
    Available since
    Jan. 4, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    164077-plasmid-map-sequence-id-320680

    Enlarge

  12. pDisplay-FRISZ

    Plasmid
    #176887
    Purpose
    Far-red fluorescent zinc indicator FRISZ in pDisplay
    Depositor
    Huiwang Ai
    Publication
    Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FRISZ
    Tags
    pDisplay backbone elements for export and membran…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    176887-plasmid-map-sequence-id-352278

    Enlarge

  14. pEMS1228

    Plasmid
    #29114
    Purpose
    Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested
    Depositor
    Elizabeth Simpson
    Publication
    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple246 (VIM Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    EGFP-NLS
    Available since
    Nov. 4, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    29114-plasmid-map-sequence-id-13529

    Enlarge

  19. pCK005_U6-Sp-sgRNA_UP-TdTomato_WPRE

    Plasmid
    #85453
    Purpose
    Lentiviral CRISPR system backbone bearing BsmBI site for S. pyogenes new guide RNAs and TdTomato.
    Depositor
    Aviv Regev
    Publication
    Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do
    Insert
    U6-sgRNA-Backbone-UP-TdTomato
    Use
    CRISPR and Lentiviral
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    deletion of AA123-365 in tdTomato (please see dep…
    Available since
    April 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    85453-plasmid-map-sequence-id-173968

    Enlarge

  20. pDN43

    Plasmid
    #61346
    Purpose
    mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS11/PKIt NES)
    Depositor
    Barbara Di Ventura, Roland Eils
    Publication
    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry
    Tags
    GS linker-AsLov2-biNLS11 and PKIt NES
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61346-plasmid-map-sequence-id-102125

    Enlarge

Showing: 3121 - 3140 of 12214 results