We narrowed to 142,928 results for: ins

1. pCMV-T7-BPNLS-ABE8e(V106W)-SpCas9(D10A)-BPNLS-P2A-EGFP (CA77)

   Plasmid

   #208290

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e(V106W) A-to-G base editor with SpCas9(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e(V106W)-SpCas9-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations and V106W in TadA and nSpCas9(D10…

   Promoter

   CMV and T7

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-T7-BPNLS-ABE8e(V106W)-SpRY-BPNLS-P2A-EGFP (CA136)

   Plasmid

   #208291

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e(V106W) A-to-G base editor with SpRY(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e(V106W)-SpRY-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations and V106W in TadA and nSpRY(D10A/…

   Promoter

   CMV and T7

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLV-RnSyt1-sgRNA2-dCas9-KRAB-TagBFP2 (identifier AAAA-0246)

   Plasmid

   #202555

   Purpose

   Synaptotagmin-1 sgRNA2 with TagBFP2

   Depositor

   Frederic Meunier

   Article

   Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

   Insert

   CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCAGTACTCGCGTGCCTCGCACCGG) (Syt1 Rat)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLV-RnSyt1-sgRNA3-dCas9-KRAB-TagBFP2 (Identifier AAAA-0247)

   Plasmid

   #202556

   Purpose

   Synaptotagmin-1 sgRNA3 with TagBFP2

   Depositor

   Frederic Meunier

   Article

   Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

   Insert

   CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCTCCTCCTGCAGCGGCAGCATCGG) (Syt1 Rat)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLV-RnSyt1-sgRNA1-dCas9-KRAB-TagBFP2 (identifier AAAA-0245)

   Plasmid

   #202554

   Purpose

   Synaptotagmin-1 sgRNA1 with TagBFP2

   Depositor

   Frederic Meunier

   Article

   Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

   Insert

   CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ -CACCGCGTGCCTCGCACCGGTCCGCGG) (Syt1 R. norvegius (gRNA))
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p21(dPCNA)-mCerulean-NeoR

   Plasmid

   #215082

   Purpose

   Expressesp21(dPCNA)-mCerulean in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CDKN1A (CDKN1A Human)
   

   Use

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   p21(dPCNA) denotes mutations M147A, D149A, F150A.

   Promoter

   CMV

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p21(RxL)-mCerulean-NeoR

   Plasmid

   #215081

   Purpose

   Expresses p21(RxL)-mCerulean in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CDKN1A (CDKN1A Human)
   

   Use

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   p21(RxL) denotes mutations: R19A, R20A, L21A, F22A

   Promoter

   CMV

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p21(RxL)-mCerulean-PuroR

   Plasmid

   #215078

   Purpose

   Expresses p21(RxL)-mCerulean in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CDKN1A (CDKN1A Human)
   

   Use

   Retroviral

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   p21(RxL) denotes mutations: R19A, R20A, L21A, F22A

   Promoter

   LTR

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p21(dPCNA)-mCerulean-PuroR

   Plasmid

   #215079

   Purpose

   Expresses p21(dPCNA)-mCerulean in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CDKN1A (CDKN1A Human)
   

   Use

   Retroviral

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   p21(dPCNA) denotes mutations M147A, D149A, F150A.

   Promoter

   LTR

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-28b-T7-hAsCas12a-RVR(S542R/K548V/N552R)-NLS(nucleoplasmin)-6xHis (AAS1897)

    Plasmid

    #114074

    Purpose

    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-RVR(S542R/K548V/N552R) with C-terminal NLS and His-tag

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a-RVR (S542R/K548V/N552R) with C-terminal NLS and His-tag
    

    Use

    Tags

    NLS(nucleoplasmin) and 6xHis

    Expression

    Bacterial

    Mutation

    S542R, K548V and N552R

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBABEpuro-ERBB2 C299S

    Plasmid

    #40988

    Depositor

    Matthew Meyerson

    Article

    Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    C299S

    Promoter

    Available since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

    Plasmid

    #107943

    Purpose

    Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
    

    Use

    Tags

    3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R and D908A

    Promoter

    CAG

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting

    Plasmid

    #92139

    Purpose

    Targeting vector to introduce a Fucci cell cyle reporter cassette mCherry(G1)/BFP(S/G2) at the mouse TIGRE acceptor locus using puromycin selection

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    chromatin licensing and DNA replication factor 1 (GMNN, CDT1 Human)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRS314-Cef1-M175I

    Plasmid

    #234118

    Purpose

    Suppressor screen mutant in Cef2

    Depositor

    Aaron Hoskins

    Insert

    Cef1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    M175I

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRS314-Cef1-Q193P

    Plasmid

    #234117

    Purpose

    Suppressor screen mutant in Cef1

    Depositor

    Aaron Hoskins

    Insert

    Cef1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Q193P

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS314-Cef1-A37V

    Plasmid

    #234119

    Purpose

    Suppressor screen mutant in Cef3

    Depositor

    Aaron Hoskins

    Insert

    Cef1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    A37V

    Promoter

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET283xFlagPP7CPHis

    Plasmid

    #28174

    Depositor

    Kathleen Collins

    Insert

    PP7 coat protein optimized
    

    Use

    Tags

    6xhistidine and triple FLAG peptide

    Expression

    Bacterial

    Mutation

    C68A,C73A

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAc5.1C-RLuc-A90-HhR_O

    Plasmid

    #147073

    Purpose

    Insect Expression of A90-HhR

    Depositor

    Elisa Izaurralde

    Insert

    A90-HhR
    

    Use

    Tags

    Expression

    Insect

    Mutation

    A127 instead of A90 (see comments below)

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CMV500 A-ATF2

    Plasmid

    #33362

    Depositor

    Charles Vinson

    Insert

    ATF
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Dominant Negative (see comments)

    Promoter

    CMV

    Available since

    Dec. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS416-Syf1

    Plasmid

    #234113

    Purpose

    Syf1 region PCR, restriction digest and ligation into pRS416 backbone

    Depositor

    Aaron Hoskins

    Insert

    Syf1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge