We narrowed to 18,361 results for: met

1. pAct:MCP-VP64

   Plasmid

   #78904

   Purpose

   Expresses MCP:VP64 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   MS2:VP64
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   pActin (Drosophila)

   Available Since

   March 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6_(GLuc)_INT[GFPApt]

   Plasmid

   #68428

   Purpose

   Transient expression in mammalian cells of an "INT" construct_bearing the GFP aptamer, targeting the GLuc reporter. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct_bearing the GFP aptamer
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6_(GLuc)_INT[3xK-T]

   Plasmid

   #68429

   Purpose

   Transient expression in mammalian cells of an "INT" construct_bearing three kink-turns, targeting the GLuc reporter. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct_bearing three kink-turns
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. XW83

   Plasmid

   #65841

   Purpose

   MosSCI-Pmig-13::QF::SL2::mCherry

   Depositor

   Kang Shen

   Article

   Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

   Insert

   OR74A quinic acid utilization activator (qa-1F)
   

   Use

   Tags

   mCherry

   Expression

   Worm

   Mutation

   The endogenous SphI, KpnI and SacI sites in QF ar…

   Promoter

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. AB.pCCL.sin.cPPT.U6.miR-718-Decoy.hPGK.GFP.WPRE

   Plasmid

   #46632

   Depositor

   Brian Brown

   Article

   Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

   Insert

   miR-718 Decoy
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pNB691

   Plasmid

   #60135

   Purpose

   Red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   RdLuc
   

   Use

   Tags

   ClpXP degron and Nuclear localization signal (NLS…

   Expression

   Yeast

   Mutation

   Promoter

   MET17pr

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. AB.pCCL.sin.cPPT.U6.miR-211-Decoy.hPGK.GFP.WPRE

   Plasmid

   #46600

   Depositor

   Brian Brown

   Article

   Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

   Insert

   miR-211 Decoy
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

8. MR015-BAG3 Left TALEN

   Plasmid

   #49465

   Purpose

   Left TALEN for BAG3

   Depositor

   Bruce Conklin

   Article

   Miyaoka et al Nat Methods. 2014 Mar;11(3):291-3. doi:

   Insert

   TALEN
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-ybbR-HIS-10FNIII(x4)-DocI

   Plasmid

   #58712

   Purpose

   Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

   Depositor

   Hermann Gaub

   Article

   Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

   Insert

   10FNIII(x4)-DocI
   

   Use

   Tags

   HIS, HRV 3C, and ybbR

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. AB.pCCL.sin.cPPT.U6.miR-675-5p-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46631

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-675-5p Decoy
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

11. AB.pCCL.sin.cPPT.U6.miR-1306-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46585

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-1306 Decoy
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

12. MR015-PKP2 Left TALEN_NN

    Plasmid

    #49451

    Purpose

    Left TALEN for PKP2 with NN

    Depositor

    Bruce Conklin

    Article

    Miyaoka et al Nat Methods. 2014 Mar;11(3):291-3. doi:

    Insert

    TALEN
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. p416GAL1-Ssk1t-L-IFP-F[1] (Yeast)

    Plasmid

    #52897

    Purpose

    Ssk1t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    Ssk1t-L-IFP-F[1]
    

    Use

    Tags

    L-IFP-F[1]

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pRNA-U6-TuDmiR122 seedsupplemental

    Plasmid

    #35668

    Depositor

    Phillip Zamore

    Article

    Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

    Insert

    TuD miR-122seedsupplemental
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCFJ1000

    Plasmid

    #44475

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1, PuroR, peel-1, [4-3] Gateway
    

    Use

    Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAVscCBPI TuDmiR122Gpluc7xlet7BT

    Plasmid

    #35648

    Depositor

    Phillip Zamore

    Article

    Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

    Insert

    TuD miR-122
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    May 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAVscCBPITuDlet-7Gpluc7x122BT

    Plasmid

    #35650

    Depositor

    Phillip Zamore

    Article

    Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

    Insert

    TuD let-7
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    May 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMK83

    Plasmid

    #226793

    Purpose

    Integrates slr0214 (HIP1 methyltransferase) into gidB-atpI locus of E. coli DH10B

    Depositor

    David Nielsen

    Article

    Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

    Insert

    slr0214 (slr0214 Synechocystis sp. PCC 6803)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tetracycline resistance cassette promoter (consti…

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRV∆L-5Flpo

    Plasmid

    #182965

    Purpose

    The 3rd generation of rabies vector expressing Flpo recombinase

    Depositor

    Ian Wickersham

    Article

    Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    Flpo recombinase
    

    Use

    Rabies

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. GST-beta catenin tag

    Plasmid

    #186422

    Purpose

    Encodes GST fused to beta catenin tag recognized by anti-beta catenin nanobody

    Depositor

    Stephen Elledge

    Article

    Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

    Insert

    GST-beta catenin tag
    

    Use

    Tags

    6His and GST-beta catenin tag (PDRKAAVSHWQQRS)

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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