We narrowed to 15,747 results for: pET

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pET15b T7pCONS TIR-2 sfGFP

Plasmid

#154468

Purpose

pET15b with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

Depositor

Daniel Daley

Article

Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

Insert

super folder GFP

Tags

His-thrombin

Expression

Bacterial

Promoter

T7

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pET-alpha-synuclein-BiFC

Plasmid

#87855

Purpose

pETDUET-1 based vector for BiMolecular Fluorescence Complementation based assays of alpha-synuclein oligomerisation

Depositor

Dan Mulvihill

Article

Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

Inserts

alpha-synuclein-VC155
alpha-synuclein-VN154

Tags

C-terminal fragment of mVenus BiFC protein and N-…

Expression

Bacterial

Promoter

T7

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a SV40 ST full length

Plasmid

#132636

Purpose

expresses SV40 ST full length in E. coli

Depositor

Wenqing Xu

Article

Cho et al PLoS Biol. 2007 Aug;5(8):e202. doi: 10.1

Insert

SV40 small t antigen

Expression

Bacterial

Available Since

Nov. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pET23a-His-TEV-SpyCatcher3

Plasmid

#235149

Purpose

Bacterial expression of His-tagged SpyCatcher3 separated by a TEV protease recognition site.

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

SpyCatcher3

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pETM6-At4CL-CmCHS-MsCHI

Plasmid

#73394

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

At4CL
CmCHS
MsCHI

Expression

Bacterial

Promoter

T7

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pET19pps-EndoIV(Thermus Thermophilus)

Plasmid

#236048

Purpose

pET19pps-APE1(Thermus Thermophilus)

Depositor

Luis Gabriel Brieba

Article

Cordoba-Andrade et al PeerJ. 2025 Aug 13;13:e19758. doi: 10.77

Insert

Endo IV from Thermus thermophilus

Tags

His tag and Histidine tag

Expression

Bacterial

Promoter

T7 RNA polymerase

Available Since

Aug. 1, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a-FKBP-TRIF(Nterm)

Plasmid

#221334

Purpose

Overexpression of TRIF Nterm domain in bacteria

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF Nterm domain (Ticam1 Mouse)

Tags

6xHis-4xFKBP

Expression

Bacterial

Promoter

T7

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET-Impact-T4gp43(exo-)

Plasmid

#228202

Purpose

Expression in BL21(DE3) of 3' to 5' exonuclease-deficient T4 DNA polymerase D219A

Depositor

Stephen Benkovic

Insert

43

Tags

Sce VMA intein-chitin binding domain

Expression

Bacterial

Mutation

Mutated aspartate 219 to alanine (D219A), elimina…

Promoter

T7

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pET28b-ptetO::bac::gfp

Plasmid

#217882

Purpose

expresses the bac-BGC in bacterial cells, produces bacillamide D

Depositor

Tobias A. M. Gulder

Article

Hohmann et al Commun Biol. 2024 May 28;7(1):655. doi:

Inserts

Dehydrogenase
Non-ribosomal peptide synthetase
Aminotransferase

Expression

Bacterial

Promoter

ptetO

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-AviTag-DogTag-MBP

Plasmid

#171773

Purpose

Expresses AviTag-DogTag-Maltose binding protein in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

AviTag-DogTag-MBP

Tags

His6 and Thrombin cleavage site

Expression

Bacterial

Mutation

Peptide tag added to N-terminus of MBP via a GSGE…

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a T7pCONS TIR-1 sfGFP

Plasmid

#154463

Purpose

pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

Depositor

Daniel Daley

Article

Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

Insert

super folder GFP

Tags

His-thrombin

Expression

Bacterial

Promoter

T7

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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His6-SUMO-pET28a-EfaCas1

Plasmid

#112793

Purpose

To express enterococcus faecalis Cas1 protein in E. coli

Depositor

Ailong Ke

Article

Xiao et al Nature. 2017 Oct 5;550(7674):137-141. do

Insert

EfaCas1

Tags

His6-SUMO

Expression

Bacterial

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pET-26b-NbVHH05-HA-His

Plasmid

#171567

Purpose

Bacterial expression of HA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-HA-His)

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

NbVHH05-HA-His (UBE2J1 Human)

Tags

HA-tag/His-tag and PelB signal peptide

Expression

Bacterial

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SpdCas9-SBiT_Strep-Tag-II

Plasmid

#198750

Purpose

Expresses S. Pyogenes dCas9 fused to SmallBiT fragment of split NanoLuc luciferase

Depositor

Maarten Merkx

Article

van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

Insert

SpdCas9 - SmallBiT

Tags

Strep-tag II

Expression

Bacterial

Mutation

D10A, H840A (nuclease inactivating mutations in &…

Promoter

T7

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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PET-eSpCas9-NLS-6xHis

Plasmid

#207379

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

eSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

K848A, K1003A, R1060A

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pET28-MHL: nsp10_SARS-CoV-2

Plasmid

#167851

Purpose

Bacterial expression of the SARS-CoV-2 protein nsp10

Depositor

Cheryl Arrowsmith

Article

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp10

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 16, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pET15b SARS-CoV PLpro

Plasmid

#61693

Purpose

inducible expression of SARS PLpro protease

Depositor

Michael Johnson

Article

Lee et al ChemMedChem. 2013 Aug;8(8):1361-72. doi:

Insert

SARS-CoV PLpro

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pET24a-VHH-tev-mCherry

Plasmid

#117752

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP, tev cleavage site and His6 epitope

Depositor

Martin Spiess

Article

Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37

Insert

anti-GFP nanobody fused to a T7, tev site, mCherry, HA, BAP and His6 epitope

Tags

BAP, HA, His6, and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pET-24a-ELP[V-150]

Plasmid

#67015

Purpose

Expresses ELP[V-150] in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

ELP[V-150]

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pET23b-Casp3 C163A-His

Plasmid

#11822

Depositor

Guy Salvesen

Article

Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

Insert

Caspase 3 catalytic mutant (CASP3 Human)

Tags

6xHis

Expression

Bacterial

Mutation

C163A

Available Since

May 12, 2006

Availability

Academic Institutions and Nonprofits only