We narrowed to 34,848 results for: CaS;

1. pINDUCER dCas9-TET1CD

   Plasmid

   #101921

   Purpose

   The dCas9-TET1CD fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948).

   Depositor

   Danwei Huangfu

   Article

   Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

   Insert

   dCas9-TET1CD
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9(BB)-2A-Puro-C9orf72

   Plasmid

   #83439

   Purpose

   Expresses Cas9 and a gRNA targeting C9orf72

   Depositor

   Shawn Ferguson

   Article

   Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

   Insert

   gRNA targeting human C9orf72 (C9orf72 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. CRISPR-SP-Cas9 reporter

   Plasmid

   #62733

   Purpose

   Mammalian CRISPR-SP-Cas9 reporter

   Depositor

   Niels Geijsen

   Article

   D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

   Insert

   CRISPR-SP-Cas9 target seq + tdTomato (out of frame)
   

   Use

   CRISPR and Lentiviral

   Tags

   CRISPR-SP-Cas9 target seq (hAAVS1 locus) and HA t…

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-Alpha

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pINDUCER dCas9-TET1CDMut

   Plasmid

   #101920

   Purpose

   The dCas9-TET1CDMut fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948). The TET1 catalytic domain is mutated to abolish catalytic function.

   Depositor

   Danwei Huangfu

   Article

   Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

   Insert

   dCas9-TET1CD Mut
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Mutated TET1 catalytic domain

   Promoter

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHS638_Flex-Cas12a-CBE

   Plasmid

   #244845

   Purpose

   Mammalian expression of Flex-Cas12a cytosine base editor

   Depositor

   Jennifer Doudna

   Article

   Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

   Insert

   Flex-dCas12a-CBE
   

   Use

   CRISPR

   Tags

   2xSV40 NLS-APOBEC3A and Nucleoplasmid NLS-UGI-SV4…

   Expression

   Mammalian

   Mutation

   G146R/R182V/D535G/S551F/D665N/E795Q/D832A

   Promoter

   CMV

   Available Since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. 138-dCas9-Dnmt3a

   Plasmid

   #84570

   Purpose

   PiggyBac transposon system construct with dCas9-Dnmt3a

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

   Insert

   dCas9-Dnmt3a (DNMT3A Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCF140_Gag-SpyCas9-NLS

   Plasmid

   #225959

   Purpose

   CMV-Intron-Gag-SpyCas9-NLS. Expresses Gag-SpyCas9-NLS for VLP production.

   Depositor

   Christof Fellmann

   Article

   Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

   Insert

   CMV-Intron-Gag-SpyCas9-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6-PspCas13b-GEMSgRNA2

   Plasmid

   #204768

   Purpose

   pU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.

   Depositor

   Kate Meyer

   Article

   Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   guide RNA targeting GEMS m6A sensor mRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-dCas9-VP64

   Plasmid

   #47107

   Purpose

   Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Insert

   dCas9-VP64
   

   Use

   CRISPR

   Tags

   Flag, HA, SV40 NLS, and VP64

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive)

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pdestMB14_sur5p-dCas9-VP64

    Plasmid

    #177788

    Purpose

    Overexpression of dCas9-VP64 in C. elegans

    Depositor

    Michael Ristow

    Article

    Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

    Insert

    dCas9-VP64
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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