We narrowed to 48,920 results for: ang

1. pNSSCP

   Plasmid

   #183188

   Purpose

   PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; DivIVA-sfGFP expressed under T

   Depositor

   Hsiao-Chun Huang

   Article

   Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1

   Inserts

   DivIVA and superfolder GFP fusion protein
   SpmX450 and T7 181+ fusion protein
   T7 RNAP 1-179 variant and SpmX450 fusion protein
   mRFP1 and popZ fusion protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiCRISPR v2-sgNCKAP1-2

   Plasmid

   #210134

   Purpose

   knock out NCKAP1 in mammalian cells

   Depositor

   Boyi Gan

   Article

   Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

   Insert

   Nck-associated protein 1 (NCKAP1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. lentiCRISPR v2-sgNCKAP1-1

   Plasmid

   #210133

   Purpose

   knock out NCKAP1 in mammalian cells

   Depositor

   Boyi Gan

   Article

   Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

   Insert

   Nck-associated protein 1 (NCKAP1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. p2T-CMV-miniSdd6-BE4max-BlastR

   Plasmid

   #204850

   Purpose

   For cytosine base editing using miniSdd6 in mammalian cells

   Depositor

   Caixia Gao

   Article

   Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

   Insert

   miniSdd6-nSpCas9(D10A)-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A in SpCas9

   Promoter

   CMV, SV40, EM7

   Available since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3 myc card9 mutant

   Plasmid

   #16254

   Depositor

   William Kaelin

   Article

   Yang et al Mol Cell. 2007 Oct 12. 28(1):15-27.

   Insert

   card9 mutant (CARD9 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   S528A, T531A, T533A, S536A

   Promoter

   Available since

   Nov. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3.1+-PAFAH1B1_R22-

   Plasmid

   #92431

   Purpose

   mammalian expression of PAFAH1B1 R22 mutant. The protein is truncated and non functional (negative control).

   Depositor

   Ralf Brandes, Matthias Leisegang

   Article

   Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

   Insert

   PAFAH1B1 (PAFAH1B1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R22 (stop codon in position 22)

   Promoter

   CMV

   Available since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3-U6-pegRNA_KCNA1-EGFP

   Plasmid

   #159787

   Purpose

   S. pyogenes pegRNA for C to A mutation at the KCNA1 site of human cells using prime editing

   Depositor

   Xiaolong Wang

   Article

   Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

   Insert

   spacer of pegRNA targeting KCNA1 gene (KCNA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGL3-U6-sgRNA_KCNA1-mcherry

   Plasmid

   #159788

   Purpose

   S. pyogenes sgRNA collocated with pegRNA targeting human KCNA1 gene

   Depositor

   Xiaolong Wang

   Article

   Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

   Insert

   spacer of sgRNA targeting KCNA1 gene (KCNA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMSCV-PIG-Lin28b

   Plasmid

   #64795

   Purpose

   Expresses human Lin28b in mammalian cells

   Depositor

   Joshua Mendell

   Article

   Chang et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

   Insert

   Lin28b (LIN28B Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX-4T-1-Dicer

    Plasmid

    #171543

    Purpose

    Encodes a Dicer fragment extending from from R1841 to the C-terminus

    Depositor

    Yan Zeng

    Article

    Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

    Insert

    DICER1 (DICER1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Tol2-LyzC-Rac-WT(guide resistant)-2a-mcherry-U6ac-Rac guides

    Plasmid

    #168242

    Purpose

    "Rescue Rac expression in neutrophil specific knockout"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    Rac(guide resistant)-WT
    

    Use

    Zebrafish expression

    Tags

    mcherry

    Expression

    Mutation

    Promoter

    LyzC

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-puro-N-Flag-Snrnp40

    Plasmid

    #134253

    Purpose

    Lentivector encoding Flag-tagged Snrnp40

    Depositor

    Bruce Beutler

    Article

    Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

    Insert

    Snrp40 (Snrnp40 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBT346.6-H3.3-K4M-bGHpolyA

    Plasmid

    #128743

    Purpose

    Inducible expression of histone H3.3K4M-FLAG/HA

    Depositor

    Kai Ge

    Article

    Jang et al Nucleic Acids Res. 2019 Jan 25;47(2):607

    Insert

    H3.3K4M (H3-3A Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    K4M

    Promoter

    CAG

    Available since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWPXL-p150-MIRΔ

    Plasmid

    #160995

    Purpose

    Express mouse p150 protein with MIR deletion mutation

    Depositor

    Zhiguo Zhang

    Article

    Cheng et al Nucleic Acids Res. 2019 Dec 2;47(21):111

    Insert

    CHAF1A (Chaf1a Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    MIR (HP1-interacting domain) deletion

    Promoter

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWPXL-p150-PIP2Δ

    Plasmid

    #160994

    Purpose

    Express mouse p150 protein with PIP2 deletion mutation

    Depositor

    Zhiguo Zhang

    Article

    Cheng et al Nucleic Acids Res. 2019 Dec 2;47(21):111

    Insert

    CHAF1A (Chaf1a Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PIP2 motif deletion

    Promoter

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSpCas9(BB)-2A-Puro-Exon4-PAFAH1B1

    Plasmid

    #92433

    Purpose

    expresses Cas9 and gRNA targeting PAFAH1B1

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

    Insert

    PAFAH1B1 (PAFAH1B1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSpCas9(BB)-2A-Intron4-PAFAH1B1

    Plasmid

    #92434

    Purpose

    expresses Cas9 and gRNA targeting PAFAH1B1

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

    Insert

    PAFAH1B1 (PAFAH1B1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL3-U6-pegRNA_HOXD13_CtoT-EGFP

    Plasmid

    #159792

    Purpose

    S. pyogenes pegRNA for C to T mutation at the HOXD13 site of mouse cells using prime editing

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

    Insert

    spacer of pegRNA_HOXD13_CtoT targeting HOXD13 gene (HOXD13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL3-U6-pegRNA_HOXD13_CtoG-EGFP

    Plasmid

    #159791

    Purpose

    S. pyogenes pegRNA for C to G mutation at the HOXD13 site of mouse cells using prime editing

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

    Insert

    spacer of pegRNA_HOXD13_CtoG targeting HOXD13 gene (HOXD13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GFP-DroshaS300A

    Plasmid

    #62526

    Purpose

    Serine 300 of Drosha is mutated to alanine

    Depositor

    Bharat Ramratnam

    Article

    Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

    Insert

    human Drosha with serine 300 changed to alanine (DROSHA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    changed serine 300 to alanine

    Promoter

    Available since

    March 20, 2015

    Availability

    Academic Institutions and Nonprofits only