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We narrowed to 18,759 results for: nls

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Showing: 3161 - 3180 of 18759 results

  1. pCMV-ABE8eWQ

    Plasmid
    #161815
    Purpose
    Expresses ABE8eWQ in mammalian cells
    Depositor
    Sangsu Bae
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-TadA8e(V106W/D108Q)-32AA linker-hSpCas9(D10A)-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Available since
    Jan. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    161815-plasmid-map-sequence-id-320654

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  2. pRN3P-NLS-EGFP-Btgh-3NLS

    Plasmid
    #194469
    Purpose
    Encodes for bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.
    Depositor
    Mathieu Boulard
    Publication
    Formichetti et al Dev Cell 10 Jan 2024
    Insert
    BtGH84
    Use
    For in vitro transcription from t3 promoter after…
    Tags
    SV40 Nuclear Localization Signal, SV40 Nuclear Lo…
    Expression
    Bacterial
    Available since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    194469-plasmid-map-sequence-id-384873

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  3. NLS-dCas9-HA-NLS-GFP-VP16

    Plasmid
    #183923
    Purpose
    VP16 activation domain coupled to catalytically inactive dCas9 for localization; labeled with GFP
    Depositor
    Karsten Rippe
    Publication
    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-GFP-VP16
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    June 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    183923-plasmid-map-sequence-id-364153

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  7. pMini-CMV-NLS-dead R-IscB (D60A, H269A)-NLS_T2A_mCherry_U6-ωRNA

    Plasmid
    #246430
    Purpose
    All-in-one plasmid. Expresses R-IscB in mammalian cells. Dead mutations included.
    Depositor
    Ailong Ke
    Publication
    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    O.gue IscB with dead mutations (D60A, H269A) and delta-TID
    mCherry
    ωRNA
    Tags
    SV40 NLS, Thosea asigna virus 2A peptide, and nuc…
    Expression
    Mammalian
    Mutation
    changed Aspartic Acid 60 to Alanine, changed Hist…
    Promoter
    CMV and U6
    Available since
    Oct. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    246430-plasmid-map-sequence-id-490873

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  9. pcDNA4/TO-myc NES-mCE(K294A) NLS-Flag

    Plasmid
    #82469
    Purpose
    Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme, inactive form
    Depositor
    Daniel Schoenberg
    Publication
    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d
    Insert
    mRNA capping enzyme (Rngtt Mouse)
    Tags
    Flag and Myc
    Expression
    Mammalian
    Mutation
    changed Lysine 294 to Alanine
    Promoter
    CMV
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    82469-plasmid-map-sequence-id-163073

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  14. pLV_hPGK-2xMeCP2NLS-dCas9-HA-2xSV40NLS-KOX1-NpNLS-P2A/T2A-mTagBFP2-2xMycNLS-WPRE

    Plasmid
    #254799
    Purpose
    Constituitve expression of dCas9-KOX1 with optimized nuclear localization in iPSC-derived neurons via lenti delivery
    Depositor
    Ophir Shalem
    Publication
    Cajka et al bioRxiv [Preprint]. 2024 Oct 19:2024.10.
    Insert
    2xMeCP2NLS-dCas9-HA-2xSV40NLS-KOX1-NpNLS-P2A/T2A-mTagBFP2-2xMycNLS
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    catalytically dead spCas9
    Promoter
    hPGK
    Available since
    Aug. 31, 2026
    Availability
    Academic Institutions and Nonprofits only
    254799-plasmid-map-sequence-id-520123

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  15. pBZ202-pU6-sgBFP-An_CBh-sv40NLS-Cas9-Sa163RT-NLS-T2A-mCherry

    Plasmid
    #170184
    Purpose
    Expression vector for a sgRNA against BFP and spCas9-Sa163 RT fusion protein linked to mCherry via a T2A peptide. Donor template An was inserted in the Retron Sa163 msr-msd region by SpeI and AvrII.
    Depositor
    Hunter Fraser
    Publication
    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Sa163 RT
    Retron Sa163 msr-msd
    BFP-to-GFP conversion donor template An
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Promoter
    CBh and hU6
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    170184-plasmid-map-sequence-id-342530

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  18. pmCherry-3xNLS-OptoJNKi-dsm

    Plasmid
    #89743
    Purpose
    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11
    Depositor
    Michael Courtney
    Publication
    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10
    Insert
    NLS-OptoJNKi dark-state mutant
    Use
    Fluorescent protein
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    C450A mutation of AsLov2Jalpha
    Promoter
    CMV
    Available since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    89743-plasmid-map-sequence-id-192503

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  19. pmCherry-3xNLS-OptoJNKi-lsm

    Plasmid
    #89742
    Purpose
    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11
    Depositor
    Michael Courtney
    Publication
    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10
    Insert
    NLS-OptoJNKi lit-state mutant
    Use
    Fluorescent protein
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    I539E mutation of AsLov2Jalpha
    Promoter
    CMV
    Available since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    89742-plasmid-map-sequence-id-192502

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Showing: 3161 - 3180 of 18759 results