We narrowed to 6,087 results for: nls

1. Sniper2P

   Plasmid

   #193857

   Purpose

   Expresses human codon-optimized Sniper2P and blasticidin resistance: EFS promoter-Sniper2P-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Chem Biol. 2023 Mar 9. doi: 10.1038/

   Insert

   Sniper2L
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   E1007L

   Promoter

   EFS

   Available since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC1-ND-Cdt1-mCitrine

   Plasmid

   #193771

   Purpose

   Human Cdt1 (SV40 NLS localization and C-terminal mCitrine tag) with mutations which abroggate its S phase degradation

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   ND-Cdt1-mCitrine (CDT1 Human)
   

   Use

   Tags

   mCitrine

   Expression

   Mammalian

   Mutation

   amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…

   Promoter

   CMV

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHA853 - mec-4p::Fynempty

   Plasmid

   #139210

   Purpose

   Control vector for Fyn kinase expression in mec-4 neurons

   Depositor

   Anne Hart

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   Fyn (FYN Human)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   deletion and frame shift to prevent Fyn expression

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHA851 - osm-10p::Fynempty

   Plasmid

   #139208

   Purpose

   Control vector for Fyn kinase expression in osm-10 neurons

   Depositor

   Anne Hart

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   Fyn (FYN Human)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   deletion and frame shift to prevent Fyn expression

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. FUS_LC_charge_RtoK

   Plasmid

   #139127

   Purpose

   express FUS LC with hnRNPA2-like charge patterning where all R are changed to K

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Add K and D to FUS LC so it has the same charge p…

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FUS_LC_charge

   Plasmid

   #139126

   Purpose

   express FUS LC with an hnRNPA2 LC-like charge patterning

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Add R, K, D to make FUS LC have similar charge pa…

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHJ4

   Plasmid

   #158785

   Purpose

   Pan-neuronal nuclear-localized GCaMP expression in C. elegans

   Depositor

   Zachary Knight, Jennifer Garrison

   Article

   Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

   Insert

   Prab-3::nls::GCaMP6m
   

   Use

   Tags

   NLS

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. hnRNPA2_LC_WT_S285C

   Plasmid

   #98665

   Purpose

   Expresses 6xHis-tagged hnRNPA2 AA 190-341 with S285C

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

   Insert

   hnRNPA2 (HNRNPA2B1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   S285C

   Promoter

   Available since

   Jan. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. hnRNPA2_LC_D290V_S285C

   Plasmid

   #98666

   Purpose

   Expresses 6xHis-tagged hnRNPA2 AA 190-341 with S285C and D290V

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

   Insert

   hnRNPA2 (HNRNPA2B1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   S285C D290V

   Promoter

   Available since

   Jan. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. hnRNPA2_LC_WT_S329C

    Plasmid

    #98667

    Purpose

    Expresses 6xHis-tagged hnRNPA2 AA 190-341 with S329C

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S329C

    Promoter

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. hnRNPA2_LC_D290V_S329C

    Plasmid

    #98668

    Purpose

    Expresses 6xHis-tagged hnRNPA2 AA 190-341 with D290V and S329C

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D290V S329C

    Promoter

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. hnRNPA2_LC_D290V

    Plasmid

    #98658

    Purpose

    Expresses 6xHis-tagged hnRNPA2 AA 190-341 with D290V

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D290V

    Promoter

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. hnRNPA2_LC_D220V

    Plasmid

    #98659

    Purpose

    Expresses 6xHis-tagged hnRNPA2 AA 190-341 with D220V

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D220V

    Promoter

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. hnRNPA2_LC_D230V

    Plasmid

    #98660

    Purpose

    Expresses 6xHis-tagged hnRNPA2 AA 190-341 with D230V

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D230V

    Promoter

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. hnRNPA2_LC_P298L

    Plasmid

    #104465

    Purpose

    express His tagged P298L hnRNPA2 LC

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    P298L

    Promoter

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MBP-hnRNPA2_LC_R191K_R254K

    Plasmid

    #104466

    Purpose

    express MBP hnRNPA2 LC with 2 R to K mutations

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    R191K R254K

    Promoter

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. hnRNPA2_LC_P298L_S329C

    Plasmid

    #104470

    Purpose

    express His tagged P298L S329C hnRNPA2 LC

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    P298L S329C

    Promoter

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. BPK4393 - human expression plasmid for SpCas9 Cluster 5 + Q926A

    Plasmid

    #101192

    Purpose

    Human expression plasmid for SpCas9 Cluster 5 + Q926A variant: CMV-T7-hSpCas9-Cluster5+Q926A(K918A, V922A, R925A, Q926A)-NLS(SV40)-3xFLAG

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster5+Q926A(K918A/V922A/R925A/Q926A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    K918A/V922A/R925A/Q926A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MMW3629 - human expression plasmid for SpCas9 Cluster 3 + Q926A

    Plasmid

    #101188

    Purpose

    Human expression plasmid for SpCas9 Cluster 3 + Q926A variant: CMV-T7-hSpCas9-Cluster3+Q926A(T657A, G658A, W659A, R661A, Q926A)-NLS(SV40)-3xFLAG

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster3+Q926A(T657A/G658A/W659A/R661A/Q926A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    T657A/G658A/W659A/R661A/Q926A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MMW3759 - human expression plasmid for SpCas9 Cluster 4 + Q926A

    Plasmid

    #101190

    Purpose

    Human expression plasmid for SpCas9 Cluster 4 + Q926A variant: CMV-T7-hSpCas9-Cluster4+Q926A(F491A, M495A, T496A, N497A, Q926A)-NLS(SV40)-3xFLAG

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster4+Q926A(F491A/M495A/T496A/N497A/Q926A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    F491A/M495A/T496A/N497A/Q926A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge