We narrowed to 16,535 results for: puromycin

1. pMXs-puro GFP-p62 D337, 338, 339A

   Plasmid

   #38280

   Depositor

   Noboru Mizushima

   Article

   Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

   Insert

   Sequestosome-1 (Sqstm1 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   changed D337A, D338A, D339A

   Available Since

   Sept. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pLPCX-NICD

   Plasmid

   #44471

   Depositor

   Ernesto Canalis

   Article

   Zanotti et al J Biol Chem. 2011 Feb 11;286(6):4576-88.

   Insert

   Notch intracellular domain (Notch1 Mouse)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Contains amino acid 1753 to C-Term

   Promoter

   CMV

   Available Since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pKIBra-P2A-mCherry-Puro-sfGFP

   Plasmid

   #115686

   Purpose

   Knock-in of P2A-mCherry at the mouse Brachyury (T) gene

   Depositor

   David Suter

   Article

   Deluz et al Genes Dev. 2016 Nov 15;30(22):2538-2550.

   Insert

   P2A-mCherry-loxP-PGK-Puro-sfGFP-loxP
   

   Expression

   Mammalian

   Available Since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-C-ultraID-Puro

   Plasmid

   #207726

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. Flag pLJM1 RagB 54L

   Plasmid

   #19314

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   Ras-related GTP binding B (RRAGB Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   T54L

   Available Since

   Oct. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMA3287

   Plasmid

   #46883

   Purpose

   Lentiviral vector encoding Tet-inducible mitochondrially targeted Y147A mutant human Uracil-N-glycosylase

   Depositor

   Mikhail Alexeyev

   Article

   Shokolenko et al DNA Repair (Amst). 2013 Jul;12(7):488-99

   Insert

   Uracil-N-glycosylase, Y147A mutant (UNG Human)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Y147A

   Promoter

   TRE-Tight

   Available Since

   Aug. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-N-Puro-mNeon

   Plasmid

   #207687

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mNeon cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMXs-puro GFP-p62 dC

   Plasmid

   #38282

   Depositor

   Noboru Mizushima

   Article

   Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

   Insert

   Sequestosome-1 (Sqstm1 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Truncate mutant 1-265 amino acids residues

   Available Since

   Aug. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-N-Puro-3xFLAG

   Plasmid

   #207695

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-3xFLAG cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. PB-Ostir-puro

    Plasmid

    #161972

    Purpose

    Expresses Ostir and puromycin resistance gene in mammalian cells

    Depositor

    Joanna Wysocka

    Article

    Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

    Insert

    Ostir-3xHA-p2a-puroR
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEBTet_NAD-Snifit-mito

    Plasmid

    #113916

    Purpose

    Plasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

    Insert

    NAD-Snifit
    

    Expression

    Mammalian

    Promoter

    CMV TetO2

    Available Since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCas9/VRQR-sgRNA (BbsI)

    Plasmid

    #129725

    Purpose

    Expressing SpCas9/VRQR mutant and sgRNA for target sequence with NGA PAM

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLentiCMV Puro DEST ERKKTRrsFastLime

    Plasmid

    #205765

    Purpose

    Expression of ERK KTR rsFastLime under CMV promoter (With Puromycin Resistance)

    Depositor

    Edward Boyden

    Article

    Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

    Insert

    ERK KTR rsFastLime
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    wt

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. p663-UBC-miniTurbo-V5-CDKL5_IDG-K

    Plasmid

    #170578

    Purpose

    Gateway destination clone of CDKL5 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CDKL5 (CDKL5 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    E854G- please see depositor comments

    Promoter

    Ubiquitin

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Flag pLJM1 RagD wt

    Plasmid

    #19316

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    Ras-related GTP binding D (RRAGD Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Flag-HA-BRCC3

    Plasmid

    #22540

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    BRCC3 (BRCC3 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMRXIP Turquoise2-Syntaxin17

    Plasmid

    #89938

    Purpose

    Expresses Syntaxin17 tagged with Turquoise2 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Tsuboyama et al Science. 2016 Nov 25;354(6315):1036-1041

    Insert

    human Syntaxin 17 (STX17 Human)
    

    Use

    Retroviral

    Tags

    mTurquoise2

    Available Since

    June 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMRXIP-3xMyc-STX17FL

    Plasmid

    #89942

    Purpose

    Expresses syntaxin17 tagged with Myc in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Uematsu et al Autophagy. 2017 Aug 3;13(8):1452-1464. d

    Insert

    human syntaxin17 (STX17 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Available Since

    June 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMXs-c-Myc-IP

    Plasmid

    #15921

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    myelocytomatosis oncogene (Myc Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. qTAG-C-mNeon-Puro

    Plasmid

    #207720

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge