We narrowed to 600 results for: HA1

1. pDGB3_alpha1_CBS-FRT-OCS-BeYDVRepRepA-tNos (GB4646)

   Plasmid

   #225438

   Purpose

   Transcriptional unit for copper- and flipase-regulated BeYDV Rep/RepA. OCSt, flanked by FRT sites, is embedded in the 5'UTR of miniDFR.

   Depositor

   Diego Orzaez

   Insert

   CBS-FRT-OCS-BeYDVRepRepA-tNos
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. piggyBac_rtTA (4th_Gen)_SNCA_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1623)

   Plasmid

   #209085

   Purpose

   Ngn2 mediated cortical neuron induction, SNCA(wt) expression, Synapsin promoter BirA ligase expression

   Depositor

   Vikram Khurana

   Article

   Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

   Inserts

   SNCA (SNCA Human)
   NGN2 (Neurog2 Mouse)
   BirA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   TRE4G and hSyn

   Available since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDGB3 alpha1 U6-26-1gRNA-DFR 2.1 (GB1838)

   Plasmid

   #160625

   Purpose

   GB-cassette for the expression of a guide RNA targeting the DFR promoter with 2.1 Ms2 aptamer in the 3' of the scaffold

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   U6-26-1gRNA-DFR 2.1
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   U6-26

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-FLEX-mCherry-FHA1-subT391A-mCherry

   Plasmid

   #104462

   Purpose

   Cre-dependent, Non-phosphorylatable PKA consensus substrate tagged with mCherry

   Depositor

   Bernardo Sabatini

   Article

   Chen et al Neuron. 2017 Dec 6;96(5):1070-1083.e5. d

   Insert

   mCherry-subT391A-FHA1-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   CAG

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDGB3 alpha1 Tnos:nptII:Pnos - P35S:Cas9:Tnos - P35S:DsRed:Tnos (GB2234)

   Plasmid

   #160628

   Purpose

   Module for the constitutive expression of the nptII, Cas9 and DsRed genes.

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

   Insert

   tNos:nptII:PNos-P35s:Cas9:tNos-P35s:DsRed:tNos
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Pnos, 35S, 35S

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NEL-mcherry-Tnos

   Plasmid

   #211841

   Purpose

   Plant transient expression cassette for the E3-DART ligase containing a nuclear localization signal.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   LRR-NEL
   

   Use

   Synthetic Biology

   Tags

   3xNLS-HA and mCherry

   Expression

   Plant

   Mutation

   Promoter

   UBIQUITIN10

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTub-alpha1-EB3-NeonGreen-T2A-iLifeact-mCherry

   Plasmid

   #175261

   Purpose

   labelling of microtubule plus end and F-actin structures

   Depositor

   Sebastián Dupraz, Frank Bradke

   Article

   Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

   Insert

   EB3-NeonGreen-T2A-iLifeact-mCherry (MAPRE3 Human)
   

   Use

   Tags

   mCherry (on iLifeact) and mNeonGreen (on EB3)

   Expression

   Mammalian

   Mutation

   None (wt)

   Promoter

   pTub-alpha1

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1628)

   Plasmid

   #209086

   Purpose

   Ngn2 mediated cortical neuron induction, SNCA(Avi tagged) expression, Synapsin promoter BirA ligase expression

   Depositor

   Vikram Khurana

   Article

   Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

   Inserts

   SNCA (SNCA Human)
   NGN2 (Neurog2 Mouse)
   BirA
   

   Use

   Tags

   AviTag

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   TRE4G and hSyn

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDGB3 alpha1 U6-26:gRNA 4 (Pnos):2.1 aptamer (GB1724)

   Plasmid

   #160621

   Purpose

   Target for the Nopaline Synthetase Promoter with the MS2 recognition 2.1 loop in position3' in the scaffold

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   U6-26:gRNA 4 (pNos): 2.1 aptamer
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   U6-26

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDGB3_alpha1_LIR1-35S-eGFP-T35S-SIR-LIR2 (GB4312)

    Plasmid

    #225435

    Purpose

    BeYDV LIR1 + TU 35S:eGFP:T35S + BeYDV SIR-LIR2.

    Depositor

    Diego Orzaez

    Insert

    LIR1:35S:eGFP:T35S-SIR:LIR2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge