Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 1,212 results for: Ncl

Clear keywords
Showing: 451 - 480 of 1212 results

  1. pLenti Lifeact-mRuby2 PuroR

    Plasmid
    #84386
    Purpose
    Lentiviral expression of mRuby2-tagged Lifeact with Puromycin selection in cells including neurons
    Depositor
    Ghassan Mouneimne
    Insert
    Lifeact
    Use
    Lentiviral
    Tags
    mRuby2
    Promoter
    CMV immediate early
    Available Since
    Jan. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    84386-plasmid-map-sequence-id-272895

    Enlarge

  2. pSLfa[Tc'hsp5'3'UTR]fa

    Plasmid
    #86450
    Purpose
    Heat shock-inducible promoter including hsp-UTRs, ubiquitous expression upon heat-shock of any inserted gene of interest in Tribolium; (Shuttle plasmid)
    Depositor
    Gregor Bucher
    Insert
    Tc'hsp5'3'UTR
    Expression
    Insect
    Available Since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    86450-plasmid-map-sequence-id-171108

    Enlarge

  3. pCI-SMN2-skipping-GLuc

    Plasmid
    #218670
    Purpose
    Alternative splicing signaling: signal decrease with Ex7 inclusion increasing
    Depositor
    Jingxin Wang
    Insert
    SMN2 (SMN2 )
    Expression
    Mammalian
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    218670-plasmid-map-sequence-id-450976

    Enlarge

  4. pKP1469

    Plasmid
    #160815
    Purpose
    Expression of ExbB protein; the exbB gene replaces tonB gene in BamHI site of pKP325; includes pBAD promoter and optimized RBS from pBAD24-derived pKP660 (ExbB, ExbD)
    Depositor
    Kathleen Postle
    Insert
    exbB
    Expression
    Bacterial
    Available Since
    Nov. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    160815-plasmid-map-sequence-id-316117

    Enlarge

  5. pENTR CMVie-Lifeact-mTagBFP2 L1-R5

    Plasmid
    #84388
    Purpose
    Multisite Gateway entry vector for expression of mTagBFP2-tagged Lifeact in cells including neurons
    Depositor
    Ghassan Mouneimne
    Insert
    Lifeact
    Use
    Gateway
    Tags
    mTagBFP2
    Expression
    Mammalian
    Promoter
    CMV immediate early
    Available Since
    Jan. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    84388-plasmid-map-sequence-id-169692

    Enlarge

  6. pUPD2 MS2 (GB2665)

    Plasmid
    #160597
    Purpose
    CDS of MS2 protein with B3 nomenclature
    Depositor
    Diego Orzaez
    Insert
    MS2
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160597-plasmid-map-sequence-id-322069

    Enlarge

  7. 2xUAS-1xhsp70-attB

    Plasmid
    #220317
    Purpose
    includes 2 UAS cassettes and 1 heat-shock inducible hsp70 casette
    Depositor
    Erdem Bangi
    Type
    Empty backbone
    Expression
    Insect
    Available Since
    Aug. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    220317-plasmid-map-sequence-id-442487

    Enlarge

  8. pENTR CMVie-Lifeact-mCardinal L1-R5

    Plasmid
    #84392
    Purpose
    Multisite Gateway entry vector for expression of mCardinal-tagged Lifeact in cells including neurons
    Depositor
    Ghassan Mouneimne
    Insert
    Lifeact
    Use
    Gateway
    Tags
    mCardinal
    Expression
    Mammalian
    Promoter
    CMV immediate early
    Available Since
    Jan. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    84392-plasmid-map-sequence-id-169693

    Enlarge

  9. pUPD2 dCas9 (GB2664)

    Plasmid
    #160596
    Purpose
    CDS of dCas9 with B3 nomenclature
    Depositor
    Diego Orzaez
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160596-plasmid-map-sequence-id-322070

    Enlarge

  10. pICSL30069

    Plasmid
    #245571
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag Twin Streptavidin-TurboID Fusion N-TAG (includes a 3' 3xGGGS Linker)
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245571-plasmid-map-sequence-id-495565

    Enlarge

  11. mP_ILB_Vector 1

    Plasmid
    #232193
    Purpose
    The plasmid vector designed for the expression of four genes in oleaginous yeasts, including Yarrowia lipolytica and Rhodotorula toruloides.
    Depositor
    Matrika Bhattarai
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Yeast
    Available Since
    March 3, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    232193-plasmid-map-sequence-id-463610

    Enlarge

  12. pBABEneo mt T Ag

    Plasmid
    #133713
    Purpose
    Large T antigen mutant form in pBABEneo vector for mammalian expression.
    Depositor
    James A. DeCaprio
    Insert
    SV40gp6 (SV40gp6 SV40)
    Use
    Retroviral
    Mutation
    aa 1-354 of T antigen (including aa 1-130); no p5…
    Available Since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    133713-plasmid-map-sequence-id-276425

    Enlarge

  13. pGR595

    Plasmid
    #213785
    Purpose
    Integrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.
    Depositor
    Chang Liu
    Insert
    BadBoy2 TP-DNAP1
    Use
    Synthetic Biology
    Expression
    Yeast
    Available Since
    March 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    213785-plasmid-map-sequence-id-424581

    Enlarge

  14. PPT1

    Plasmid
    #25205
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PPT1_02 (PPT1 Human)
    Tags
    His
    Expression
    Insect
    Available Since
    Aug. 12, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. Optopatch2 (QuasAr2-mOrange2-P2A-CheRiff-eGFP)

    Plasmid
    #51656
    Purpose
    hsyn promoting optopatch, which includes a ChR64, GFP, Quasar2 and mOrange
    Depositor
    Adam Cohen
    Insert
    Quasar2-mOrange-Chr64-GFP
    Use
    Lentiviral
    Tags
    GFP and mOrange
    Expression
    Mammalian
    Promoter
    human synapsin
    Available Since
    March 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    51656-plasmid-map-sequence-id-319739

    Enlarge

  16. pNHA_OROV_NCP

    Plasmid
    #208937
    Purpose
    Expresses N-terminally HA-tagged Oropouche orthobunyavirus nucleocapsid protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    Nucleocapsid
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted start codon so HA included at N-terminus
    Promoter
    CMV
    Available Since
    Jan. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    208937-plasmid-map-sequence-id-418963

    Enlarge

  17. pTipi2.1-AZU_SP

    Plasmid
    #229144
    Purpose
    Allows for expression of recombinant antibodies. Includes human azurocidin signal peptide.
    Depositor
    Institute for Protein Innovation (IPI)
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    Jan. 9, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    229144-plasmid-map-sequence-id-457154

    Enlarge

  18. p5E-mitfa2.1

    Plasmid
    #81234
    Purpose
    zebrafish mitfa promoter entry vector for multisite Gateway cloning
    Depositor
    James Lister
    Insert
    mitfa promoter (mitfa Zebrafish)
    Use
    Multisite gateway entry vector
    Promoter
    mitfa, 2.1kb including 5' UTR
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    81234-plasmid-map-sequence-id-157888

    Enlarge

  19. pGR596

    Plasmid
    #213787
    Purpose
    Integrates at the CAN1 locus the BadBoy3 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette
    Depositor
    Chang Liu
    Insert
    BadBoy2 TP-DNAP1
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Mutation
    See manuscript
    Available Since
    March 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213787-plasmid-map-sequence-id-424625

    Enlarge

  20. pAAV-CAG-RLuc

    Plasmid
    #83282
    Purpose
    recombinant AAV vector packaging single-stranded Renilla Luciferase under the CAG promoter (includes WPRE element)
    Depositor
    Mark Kay
    Insert
    Renilla Luciferase
    Use
    AAV
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    83282-plasmid-map-sequence-id-158421

    Enlarge

  21. pGR618

    Plasmid
    #213786
    Purpose
    Integrates at the CAN1 locus the BB-Tv TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette
    Depositor
    Chang Liu
    Insert
    BB-Tv TP-DNAP1
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    March 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213786-plasmid-map-sequence-id-424624

    Enlarge

  22. p202 Dync1li1 wt

    Plasmid
    #26450
    Depositor
    Elizabeth Fisher
    Insert
    cytoplasmic dynein light intermediate chain 1 (Dync1li1 Mouse)
    Use
    Subcloning and sequencing
    Mutation
    N/A
    Available Since
    Nov. 8, 2010
    Availability
    Academic Institutions and Nonprofits only

  23. pFB-CT10HF-LIC

    Plasmid
    #39191
    Purpose
    SGC Baculovirus transfer vector with C-terminal His tag and FLAG tag, preceded by a TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection
    Depositor
    Nicola Burgess-Brown
    Type
    Empty backbone
    Use
    Baculovirus
    Tags
    TEV-His-FLAG
    Promoter
    polyhedrin
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    39191-plasmid-map-sequence-id-136169

    Enlarge

  24. pMSP1D1Q1

    Plasmid
    #97326
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional Q residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Michael Marty
    Insert
    MSP1D1Q1
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of Q residue after T25
    Promoter
    T7
    Available Since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97326-plasmid-map-sequence-id-182622

    Enlarge

  25. UKC1620-SUP35wt

    Plasmid
    #29730
    Depositor
    Tobias von der Haar
    Insert
    SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
    Expression
    Yeast
    Mutation
    Wild type gene including genomic sequence 955 bp …
    Available Since
    Sept. 6, 2011
    Availability
    Academic Institutions and Nonprofits only

  26. U6-sgRNA-BbsI-CMV-EGFP cloning vector

    Plasmid
    #239464
    Purpose
    sgRNA cloning vector with EGFP expression cassette. An sgRNA spacer sequence can be cloned into BbsI sites. A CMV-EGFP expression cassette is included as a reporter of transfection efficiency.
    Depositor
    Patrick Harrison
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6 and CMV
    Available Since
    Dec. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    239464-plasmid-map-sequence-id-476201

    Enlarge

  27. pNHA_OROV_NSs

    Plasmid
    #208934
    Purpose
    Expresses N-terminally HA-tagged Oropouche orthobunyavirus NSs protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    NSs
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted 69 bp at 5' end (including ATG) and …
    Promoter
    CMV
    Available Since
    Jan. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    208934-plasmid-map-sequence-id-418959

    Enlarge

  28. pHelper_TS_V2_ampR

    Plasmid
    #214467
    Purpose
    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Includes temperature sensitive origin for plasmid curing.
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Insert
    CspRecT / Bxb-1
    Use
    Synthetic Biology
    Promoter
    XylS / AraC
    Available Since
    April 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    214467-plasmid-map-sequence-id-429338

    Enlarge

  29. pcDNA3.1 CLN3-GFP

    Plasmid
    #78110
    Purpose
    Mammalian expression of human CLN3 with C terminal GFP fusion
    Depositor
    Thomas Braulke
    Insert
    CLN3 (CLN3 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 20, 2016
    Availability
    Academic Institutions and Nonprofits only

  30. pLVXPuro-mGFP-LIC2

    Plasmid
    #66601
    Purpose
    Expresses Dynein-1 LIC2 as a mGFP fusion
    Depositor
    David Stephens
    Insert
    LIC2 (DYNC1LI2 Human)
    Use
    Lentiviral
    Tags
    mGFP
    Expression
    Mammalian
    Available Since
    July 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    66601-plasmid-map-sequence-id-488640

    Enlarge

Showing: 451 - 480 of 1212 results