We narrowed to 2,923 results for: COB;

1. HIR WT

   Plasmid

   #24049

   Depositor

   Frederick Stanley

   Article

   Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

   Insert

   Insulin Receptor (INSR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNHEJ-recX-sacB

   Plasmid

   #158718

   Purpose

   CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recX.

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   recX
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pmyc

   Available Since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. SaCas9

   Plasmid

   #188494

   Purpose

   Expresses FLAG tagged SaCas9

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. AsCas12a

   Plasmid

   #188492

   Purpose

   Expresses FLAG tagged AsCas12a

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Plasmid L5 attB::Pleft* mWasabi

   Plasmid

   #169409

   Purpose

   Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mWasabi

   Depositor

   Clifton Barry III

   Article

   Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

   Inserts

   pLeft
   mWasabi
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Plasmid L5 attB::Pleft* mScarlet

   Plasmid

   #169410

   Purpose

   Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mScarlet

   Depositor

   Clifton Barry III

   Article

   Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

   Inserts

   pLeft
   mScarlet proper
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p201H Cas9

   Plasmid

   #59176

   Purpose

   Cas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

   Depositor

   Wayne Parrott

   Article

   Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

   Inserts

   Cas9
   hph
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   2x35S and StUbi-3P

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpCas9

   Plasmid

   #188489

   Purpose

   Expresses FLAG tagged SpCas9

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSUM-Kan-MCS2-gfp

   Plasmid

   #109290

   Purpose

   Expresses high levels of gfp in mycobacteria

   Depositor

   Nicolai van Oers

   Article

   Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Kanamycin

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21-Streptavidin-K121R

    Plasmid

    #89880

    Purpose

    Streptavidin that has unimpaired biotin binding after dye labeling

    Depositor

    Mark Howarth

    Article

    Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

    Insert

    Streptavidin-K121R-Glutamate_Tag
    

    Use

    Tags

    6 glutamate tag (C terminal on insert)

    Expression

    Bacterial

    Mutation

    K121R

    Promoter

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYC1640

    Plasmid

    #158719

    Purpose

    CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using zeocin as a selection marker.

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    Sth1 sgRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 5aa SunTag VP64 CLV3

    Plasmid

    #115485

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to the CLV3 locus with two guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. 5aa SunTag VP64 AP3

    Plasmid

    #115484

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to the AP3 locus with two guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSBY3_ligD-HygR

    Plasmid

    #104625

    Purpose

    CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD and hygromysin resistance gene.

    Depositor

    Sheng Yang

    Article

    Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

    Insert

    FnCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    FnCpf1 codon-optimized C.glutamicum

    Promoter

    hsp60

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBbdCas9S_Psyn-sgRNA500

    Plasmid

    #149640

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, Psyn

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMZ374

    Plasmid

    #59896

    Purpose

    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).

    Depositor

    Mikel Zaratiegui

    Article

    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

    Inserts

    Cas9
    rrk1:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Silent muation of the CspCI site

    Promoter

    adh1 and rrk1

    Available Since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYC2085

    Plasmid

    #158721

    Purpose

    CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using hygromycin as a selection marker.

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    Sth1 sgRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HIR K1030R

    Plasmid

    #24050

    Depositor

    Frederick Stanley

    Article

    Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

    Insert

    Insulin Receptor (INSR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K1030R

    Promoter

    Available Since

    Dec. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. gh22

    Plasmid

    #106695

    Purpose

    expression of gRNA targeting COSMC

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    COSMC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

20. Plasmid L5 attB::Pleft* mCherry

    Plasmid

    #169411

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mCherry

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    pLeft
    mCherry
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge