We narrowed to 423 results for: RPA;

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M30: pHR-SFFVp- HNRNPA1C::mCherry::SspB

Plasmid

#122668

Purpose

C terminal domain of hnRNPA1 (186-320) tagged with SspB and mCherry.

Depositor

Clifford Brangwynne

Article

Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

Insert

HNRNPA1 (HNRNPA1 Human)

Use

Lentiviral

Tags

mCherry-SspB

Expression

Mammalian

Mutation

Contains only amino acids 186-230

Promoter

SFFV

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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A1-LCD deltahexa

Plasmid

#169714

Purpose

Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, hexapeptide including aa 259-264 deleted

Depositor

Tanja Mittag

Article

Martin et al Science. 2020 Feb 7;367(6478):694-699. d

Insert

hnRNPA1 (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

hnRNPA1-A Low complexity domain including amino a…

Promoter

T7

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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A1-LCD+7R+12D

Plasmid

#178678

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with seven Arg residues added, twelve Glu residues added (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD+7R+12D (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

seven Arg residues added, twelve Glu residues add…

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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A1-LCD allW D262V

Plasmid

#234616

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262V with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_allW D262V (HNRNPA1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

C-terminal domain (186-320) bearing familial ALS …

Promoter

T7

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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A1-LCD allW D262N

Plasmid

#234615

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262N with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_allW D262N (HNRNPA1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

C-terminal domain (186-320) bearing familial ALS …

Promoter

T7

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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HNRNPA1-miRFP670-TRF1

Plasmid

#227384

Purpose

Adhesion module, creates 'seed' at telomere

Depositor

Clifford Brangwynne

Article

Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

Insert

Telomeric HNRNPA1 IDR (TERF1, HNRNPA1 Human)

Use

Lentiviral

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_hnRNPA1_allW

Plasmid

#224252

Purpose

Bacterial expression of N-terminally 6His-tagged hnRNPA1_allW

Depositor

Tanja Mittag

Article

Alshareedah et al Nat Phys. 2024 Sep;20(9):1482-1491. doi:

Insert

hnRNPA1_allW (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

F17W,F25W,F31W,F37W,F43W,Y52W, Y59W, F62W, F69W, …

Promoter

T7

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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A1-LCD-2R-2K+3D

Plasmid

#178680

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with two Arg residues removed, two Lys removed, three Asp added (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD-2R+2K+3D (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

two Arg residues removed, two Lys added, three As…

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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A1-LCD-9F+3Y

Plasmid

#178663

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with nine Phe residues removed, three Tyr added (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD-9F+3Y (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

nine Phe residues removed, three Tyr added

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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A1-LCD-14N+14Q

Plasmid

#178692

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with fourteen Gln residues replaced with Asn (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD-14N+14Q (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

fourteen Gln residues replaced with Asn

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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A1-LCD-10G+10S

Plasmid

#178682

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with ten Gly residues replaced with Ser (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD-10G+10S (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

ten Gly residues replaced with Ser

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
A1-LCD+7K+12D

Plasmid

#178679

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with seven Lys residues added, twelve Glu residues added (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD+7K+12D (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

seven Lys residues added, twelve Glu residues add…

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
A1-LCD-30G+30S

Plasmid

#178684

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with thirty Gly residues replaced with Ser (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD-30G+30S (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

thirty Gly residues replaced with Ser

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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A1-LCD+23G-23S

Plasmid

#178685

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Ser residues replaced with Gly (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD+23G-23S (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

twenty-three Ser residues replaced with Gly

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
A1-LCD-4R-2K+5D

Plasmid

#178681

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with four Arg residues removed, two Lys removed, five Asp added (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD-4R+2K+5D (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

four Arg residues removed, two Lys added, five As…

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
A1-LCD-9F+6Y

Plasmid

#178661

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with nine Phe residues removed, six Tyr added (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD-9F+6Y (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

nine Phe residues removed, six Tyr added

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
A1-LCD-8F+4Y

Plasmid

#178662

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with eight Phe residues removed, four Tyr added (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD-8F+4Y (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

eight Phe residues removed, four Tyr added

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
A1-LCD+7R+10D

Plasmid

#178677

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with seven Arg residues added, ten Glu residues added (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD+7R+10D (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

seven Arg residues added, ten Glu residues added

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
A1-LCD-12F+12Y

Plasmid

#178659

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD -12F+12Y (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

twelve Phe residues replaced with Tyr

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
A1-LCD-23S+23T

Plasmid

#178693

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Thr reisudes replaced with Ser (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD-23S+23T (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

twenty-three Thr reisudes replaced with Ser

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge