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pcDNA3.1-Beta3

Plasmid

#140988

Purpose

Encodes a Gbeta subunit (GNB3) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GBeta3 (GNB3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hFrataxin-HA

Plasmid

#31895

Depositor

Gino Cortopassi

Article

Shan et al Hum Mol Genet. 2007 Apr 15;16(8):929-41.

Insert

Frataxin (FXN Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-HA-CCND3

Plasmid

#172621

Purpose

Expresses HA-tagged cyclin D3 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND3 (CCND3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TEVp

Plasmid

#162599

Purpose

TEV protease under CMV promoter

Depositor

Masaharu Somiya

Article

Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

Insert

TEV protease

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hARFGAP2-HA

Plasmid

#206410

Purpose

Mammalian expression of hARFGAP2

Depositor

Hye-Won Shin, Kazuhisa Nakayama

Article

Saitoh et al J Biol Chem. 2009 May 15;284(20):13948-1

Insert

ARFGAP2 (ARFGAP2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GFP(1-10)

Plasmid

#70219

Purpose

Expresses GFP(1-10) in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Has Service

Cloning Grade DNA

Insert

GFP(1-10)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mPCSK2

Plasmid

#122673

Purpose

Expresses mouse PCSK2 in mammalian cells

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus proprotein convertase subtilisin/kexin type 2 (Pcsk2) (Pcsk2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-NEMOc

Plasmid

#189933

Purpose

Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

Depositor

Yubin Zhou

Article

Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

Insert

NEMOc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-NEMOm

Plasmid

#189930

Purpose

Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

Depositor

Yubin Zhou

Article

Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

Insert

NEMOs

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-NONO

Plasmid

#127655

Purpose

Over-express HA-tagged human NONO

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

Insert

NONO (NONO Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA PSPC1

Plasmid

#101764

Purpose

Expresses PSPC1 in mammalian cells

Depositor

Yuh-Shan Jou

Article

Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

Insert

paraspeckle component 1 (PSPC1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-RIPK3

Plasmid

#78804

Purpose

To overexpress RIPK3 in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TORCAR

Plasmid

#64927

Purpose

FRET-based biosensor for monitoring mTORC1 kinase activity.

Depositor

Jin Zhang

Article

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Insert

TORCAR (EIF4EBP1 Human, Synthetic)

Use

Tags

Cerulean and YPet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-RIPK1

Plasmid

#78834

Purpose

To overexpress RIPK1 in Mammalian Cell

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK1 (RIPK1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 - hSRSF6

Plasmid

#120419

Purpose

overexpression of human SRSF6

Depositor

Andreas Neueder, Gillian Bates

Article

Neueder et al Nat Commun. 2018 Sep 27;9(1):3955. doi:

Insert

SRSF6 (SRSF6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV immediate/early

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TORCAR(T/A)

Plasmid

#64928

Purpose

Negative-control mutant for TORCAR biosensor.

Depositor

Jin Zhang

Article

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Insert

TORCAR(T/A) (EIF4EBP1 Human, Synthetic)

Use

Tags

Cerulean and YPet

Expression

Mammalian

Mutation

4EBP1 domain contains T37A and T46A mutations.

Promoter

CMV

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Ii-OVA.I+II

Plasmid

#64596

Purpose

Expression of invariant chain fused to chicken ovalbumin 242-353 amino acids as model antigen for study of MHC class II presentation

Depositor

Martin Zenke, Sandra Diebold

Article

Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

Insert

Ovalbumin

Use

Tags

Invariant chain

Expression

Mammalian

Mutation

Only amino acids 242-353 (class I and class II ep…

Promoter

CMV

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5-CEP41_Halo

Plasmid

#135446

Purpose

Mamalian cell expression of HaloTag7 localized at the centrosome

Depositor

Kai Johnsson

Article

Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

Insert

CEP41-HaloTag7

Use

Tags

CEP41

Expression

Mammalian

Mutation

Promoter

CMV/TetO

Available since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-NKX3.1

Plasmid

#42897

Depositor

Edward Gelmann

Article

Steadman et al Nucleic Acids Res. 2000 Jun 15;28(12):23

Insert

NKX3.1 (NKX3-1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-HA AKT1

Plasmid

#78778

Purpose

To overexpress AKT1 in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

Insert

AKT1 (AKT1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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