We narrowed to 9,179 results for: sacs

1. pDRESS-mScarletI-antiFRET-P2A2-linker_noSacI

   Plasmid

   #191473

   Purpose

   Negative control for ratiometric comparison between green fluorescent proteins and sensors

   Depositor

   Dorus Gadella

   Article

   van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

   Insert

   none
   

   Use

   Tags

   His-mScarletI-antiFRETlinker-P2A

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDRESS-mTq2-antiFRET-P2A2-linker_noSacI

   Plasmid

   #191472

   Purpose

   Negative control for ratiometric comparison between red fluorescent proteins and sensors

   Depositor

   Dorus Gadella

   Article

   van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

   Insert

   none
   

   Use

   Tags

   His-mTurquoise2-antiFRETlinker-P2A

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CMV-FLEX-SaCas9-U6-sgVamp1

   Plasmid

   #159919

   Purpose

   Mutagenesis of Vamp1

   Depositor

   Larry Zweifel

   Insert

   Vamp1 (Vamp1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CMV-FLEX-SaCas9-U6-sgNtn1

   Plasmid

   #159907

   Purpose

   Mutagenesis of Netrin1

   Depositor

   Larry Zweifel

   Insert

   Netrin1 (Ntn1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CMV-FLEX-SaCas9-U6-sgDcc

   Plasmid

   #159906

   Purpose

   Mutagenesis of Dcc

   Depositor

   Larry Zweifel

   Insert

   Dcc (Dcc Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pColdII-SIRPT1-Chlorocebus sabaeus Sacsin

   Plasmid

   #78112

   Purpose

   Nt His-tag Chlorocebus sabaeus Sacsin 86-1375

   Depositor

   Kalle Gehring

   Insert

   Sacsin Chlorocebus sabaeus 86-1375
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-pCMV-dSaCas9-VP64-pU6-sgRNA

   Plasmid

   #158990

   Purpose

   Vector G encodes pAAV-pCMV-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in mammalian cells

   Depositor

   Chung Tin

   Article

   Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

   Insert

   dSaCas9-VP64
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available since

   Aug. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-pMecp2-dSaCas9-VP64-pU6-sgRNA

   Plasmid

   #158972

   Purpose

   Vector C encodes pAAV-pMecp2-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neurons

   Depositor

   Chung Tin

   Article

   Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

   Insert

   dSaCas9-VP64
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pMecp2

   Available since

   Sept. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE

   Plasmid

   #85452

   Purpose

   Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.

   Depositor

   Aviv Regev

   Article

   Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

   Insert

   U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBK1314-AAV-EFSNC-dSaCas9-KRAB-MECP2

    Plasmid

    #223159

    Purpose

    Expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB-MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    EF1a

    Available since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX601 miniCMV-SaCas9-SpA-sgRNA scaffold

    Plasmid

    #107055

    Purpose

    Backbone vector for cloning in target sgRNA for use with SaCas9 (SauCas9)

    Depositor

    Alex Hewitt

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-pMecp2-dSaCas9-KRAB-pU6-sgRNA

    Plasmid

    #158988

    Purpose

    Vector E encodes pAAV-pMecp2-dSaCas9-KRAB-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR interference in neurons

    Depositor

    Chung Tin

    Article

    Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

    Insert

    dSaCas9-KRAB
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pMecp2

    Available since

    Aug. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. 1782_pAAV-U6-Ai9-Sa-gRNA2-CB-SACas9-HA-OLLAS-spA_C

    Plasmid

    #135978

    Purpose

    gRNA against the right loxP site of the Ai9 allele

    Depositor

    William Lagor

    Insert

    Ai9 gRNA2
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    HA

    Expression

    Mutation

    Promoter

    CB

    Available since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV hUbC-dSaCas9-KRAB-T2A-PuroR

    Plasmid

    #106249

    Purpose

    Lentiviral vector with puro selection for expression of S. aureus dCas9-KRAB

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

    Insert

    dead S. aureus Cas9 KRAB T2A PuroR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA-NLS and NLS-KRAB

    Expression

    Mammalian

    Mutation

    D10A and N580A

    Promoter

    hUbC

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-PuroR

    Plasmid

    #162334

    Purpose

    Lentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistance

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    dSaCas9-KRAB
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJEP316-pAAV-U6SaCas9gRNA(SapI)-pA Empty Cassette

    Plasmid

    #113693

    Purpose

    U6 driven SaCas9 gRNA expression cassette without a gRNA. SapI can be used to clone in gRNAs.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    SaCas9 gRNA Cassete
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLJM1-Lyso-FLAG-GFP-Sac1-Cat-WT

    Plasmid

    #134645

    Purpose

    lysosome-targeted Sac1 catalytic domain wild type

    Depositor

    Roberto Zoncu

    Article

    Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

    Insert

    Sac1 catalytic domain (77-520 aa) (SACM1L Human)
    

    Use

    Lentiviral

    Tags

    FLAG, GFP, and lysosomal-tag (p18 N-terminal 1-39…

    Expression

    Mammalian

    Mutation

    contains only amino acids 77-520 (Please see depo…

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. NdeI – SacII – SpeI(NheI) - ANXA2 (23-339)

    Plasmid

    #136543

    Purpose

    Expresses ANXA2 chimeras in bacterial cells

    Depositor

    Laszlo Nyitray

    Article

    Ecsedi et al Structure. 2020 Aug 4;28(8):943-953.e4.

    Type

    Empty backbone

    Use

    Tags

    ANXA2 (23-339) and His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLJM1-Lyso-FLAG-GFP-Sac1-Cat-CS

    Plasmid

    #134653

    Purpose

    lysosome-targeted Sac1 catalytic domain C389S

    Depositor

    Roberto Zoncu

    Article

    Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

    Insert

    Sac1 catalytic domain (77-520 aa) CS mutant (SACM1L Human)
    

    Use

    Lentiviral

    Tags

    FLAG, GFP, and lysosomal-tag (p18 N-terminal 1-39…

    Expression

    Mammalian

    Mutation

    C389S and contains only amino acids 77-520 (Pleas…

    Promoter

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

    Plasmid

    #201151

    Purpose

    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Inserts

    SadCas9-KRAB
    gRNA-TRE
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mutation

    gRNA sequence: ATCAGTGATAGAGAACGTATG

    Promoter

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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