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pHAGE-RSV-tdTomato-2A-GCaMP6s

Plasmid

#80316

Purpose

Fluorescent reporter for calcium signaling

Depositor

Darrell Kotton

Article

Liberti WA et al Nat Neurosci. 2016 Oct 10. doi: 10.1038/

Inserts

tdTomato
GCaMP6s

Use

Lentiviral

Promoter

RSV

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-IKZF1-V2-V5

Plasmid

#107390

Purpose

Mammalian expression of IKZF1-V2-V5

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF1-V2 (IKZF1 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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1GFP/RNase H1 D210N

Plasmid

#174448

Purpose

Express GFP-tagged RNase H1 D210N in E. coli.

Depositor

Karlene Cimprich

Article

Crossley et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

RNase H1 (RNASEH1 Human)

Tags

His-tag, GFP

Expression

Bacterial

Mutation

D210N

Promoter

T7 promoter

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFastbac1-3FLAG-TOP2A

Plasmid

#247921

Purpose

Insect expression construct for N-terminal 3xFLAG-tagged TOP2A

Depositor

Junjie Chen, Huimin Zhang

Article

Zhang et al Sci Adv. 2023 Dec 8;9(49):eadi6681. doi:

Insert

TOP2A (TOP2A Human)

Tags

3xFLAG

Expression

Insect

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pHAGE-FGFR3

Plasmid

#116742

Purpose

Lentiviral expression of FGFR3

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FGFR3 (FGFR3 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pICH-chl-roGFP2-synORP1

Plasmid

#191701

Purpose

Ti plasmid pICH86988 harbouring chl-roGFP2-synORP1 for H2O2 detection in the plastids of plant cells.

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

Insert

chl-roGFP2-synORP1

Use

Binary agrobacterium ti plasmid

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1

Plasmid

#83842

Purpose

Fluorescent probe for S/G2 transition and G2/M transition, one of two plasmids for the FUCCI4 system

Depositor

Michael Lin

Article

Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1 (H1-0, SLBP Human, Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pGL004

Plasmid

#215336

Purpose

amyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank-Hy promoter)

Depositor

Gene-Wei Li

Type

Empty backbone

Expression

Bacterial

Promoter

hyper-pSpank

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pEThT-RvLEAM

Plasmid

#90033

Purpose

To express His-tagged RvLEAM in bacteria

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

Insert

RvLEAM

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-Sec61b-C1

Plasmid

#90994

Purpose

Expresses mCherry-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

Depositor

Jennifer Lippincott-Schwartz

Article

Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

Insert

mCherry-Sec61b (SEC61B Human, Synthetic)

Tags

mCherry (red fluorescence)

Expression

Mammalian

Promoter

CMV

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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