We narrowed to 8,027 results for: Dos;

1. CENP-B targeted Aurora B FRET sensor Kif2 substrate

   Plasmid

   #45214

   Depositor

   Michael Lampson

   Article

   Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

   Insert

   CENP-B targeted Aurora B FRET sensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   t7

   Available since

   May 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tet-O-FUW-Nkx6.1

   Plasmid

   #45846

   Depositor

   Marius Wernig

   Article

   Yang et al Nat Biotechnol. 2013 May;31(5):434-9. do

   Insert

   Nkx6.1 (Nkx6-1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tet-ON

   Available since

   July 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. RnBarr1-mVenus

   Plasmid

   #137790

   Purpose

   Visualization of beta-arrestin1

   Depositor

   Dorus Gadella

   Article

   Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

   Insert

   Rat beta-arrestin 1 (Arrb1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Tet-O-FUW-Zfp536

   Plasmid

   #45845

   Depositor

   Marius Wernig

   Article

   Yang et al Nat Biotechnol. 2013 May;31(5):434-9. do

   Insert

   Zfp536 (Zfp536 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-ON

   Available since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. Actin binding domain of Ezrin

   Plasmid

   #62381

   Purpose

   Expresses the actin binding domain of ezrin. Amino acids 552-586.

   Depositor

   Guillaume Charras

   Article

   Fritzsche et al Biophys J. 2014 Jan 21;106(2):343-53. do

   Insert

   Actin binding domain of ezrin (EZR Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains aa's 552-586

   Promoter

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR-L3-Csy4-Cas9n-EGFP-L2

   Plasmid

   #141046

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   Csy4-2A-Cas9n-2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLIK 3xFLAG-TWIST2 neo

   Plasmid

   #197936

   Purpose

   Lentiviral expression vector for an inducible 3xFLAG-tagged TWIST2

   Depositor

   Kevin Janes

   Article

   Dutta et al Genome Res. 2023 Feb 21;33(3):314-31. do

   Insert

   TWIST2 (TWIST2 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. JW617

   Plasmid

   #64809

   Purpose

   CUC3 expression in yeast

   Depositor

   Detlef Weigel

   Article

   Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

   Insert

   CUC3 (CUC3 Mustard Weed)
   

   Use

   Tags

   GAL4 DNA binding domain and Myc

   Expression

   Mutation

   Promoter

   Available since

   Aug. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. Sa-dCas9-NLS-3xFLAG/pcDNA3.1

   Plasmid

   #98041

   Purpose

   Expresses Sa-dCas9-NLS-3xFLAG in mammalian cells for enChIP analysis to purify specific genomic regions of interest

   Depositor

   Hodaka Fujii

   Article

   Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

   Insert

   Sa-dCas9-NLS-3xFLAG
   

   Use

   CRISPR

   Tags

   3xFLAG tag and NLS (nuclear localization signal)

   Expression

   Mammalian

   Mutation

   D10A + H557A

   Promoter

   CMV

   Available since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-nDsRedFL-miR9/9*/124

    Plasmid

    #177807

    Purpose

    The pre-miR-9/9* and pre-miR-124 sequences have been inserted into the EcoRV of pCAG-nDsRedFL-intron.

    Depositor

    Rikako Sanuki

    Article

    Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

    Insert

    miR-9/9*/124
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSMP-YY1_1

    Plasmid

    #36357

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    YY1 (YY1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

12. JW624

    Plasmid

    #64808

    Purpose

    CUC2 expression in yeast

    Depositor

    Detlef Weigel

    Article

    Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

    Insert

    CUC2 (CUC2 Mustard Weed)
    

    Use

    Tags

    GAL4 DNA binding domain

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX6P-1-eIF4E K119A

    Plasmid

    #199440

    Purpose

    Bacterial expression plasmids for production of recombinant GST-eIF4E K119A

    Depositor

    Shin-ichi Hoshino

    Article

    Ogami et al STAR Protoc. 2023 May 26;4(2):102340. do

    Insert

    1977 (EIF4E Human)
    

    Use

    Tags

    GST-fusion protein

    Expression

    Bacterial

    Mutation

    changed lysine 119 to alanine

    Promoter

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGImC_Empty

    Plasmid

    #92015

    Purpose

    Empty backbone, includes IRES-mCherry

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Type

    Empty backbone

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEN_TT 3xFLAG-TWIST2

    Plasmid

    #197935

    Purpose

    Gateway entry vector for an inducible 3xFLAG-tagged TWIST2

    Depositor

    Kevin Janes

    Article

    Dutta et al Genome Res. 2023 Feb 21;33(3):314-31. do

    Insert

    TWIST2 (TWIST2 Human)
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-AMPKsub-YPet-NES

    Plasmid

    #84635

    Purpose

    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    AMPKsub-YPet-NES
    

    Use

    Tags

    6xHis, Nuclear export signal (NES), T7 tag (gene …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOCC72

    Plasmid

    #118866

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEV

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease, mGFP, cleavable…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOCC119

    Plasmid

    #118884

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEV

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
    

    Use

    Tags

    HIS6-MBP, cleavable with 3C protease and mGFP, cl…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pC1-FGB (FlincG2)

    Plasmid

    #49204

    Purpose

    Fluorescent reporter for cGMP

    Depositor

    John Garthwaite

    Article

    Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

    Insert

    FlincG2 (PRKG1 Bovine, GFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHH0103_PPP1CA_Metallophos

    Plasmid

    #109934

    Purpose

    Protein expression and purification of PPP1CA_Metallophos

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    PPP1CA_Metallophos
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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