We narrowed to 7,711 results for: chloramphenicol

1. pSECRETS-A

   Plasmid

   #196986

   Purpose

   For SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).

   Depositor

   Eric Josephs

   Article

   Herring-Nicholas et al Commun Biol. 2024 Jan 12;7(1):86. doi: 1

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pltetO1

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDae069 (eMutaT7[PmCDA1] with optimized RBS)

   Plasmid

   #187621

   Purpose

   Expresses eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

   Depositor

   Seokhee Kim

   Article

   Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

   Insert

   PmCDA1-T7RNAP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pmMaple3-CAM

   Plasmid

   #101148

   Purpose

   Fluorescent protein for superresolution imaging

   Depositor

   Xiaowei Zhuang

   Article

   Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

   Insert

   E. coli codon optimized mMaple3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pACλCI-β-flap (831-1057)

   Plasmid

   #53733

   Purpose

   Expression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.

   Depositor

   Ann Hochschild

   Article

   Dove et al Nature. 1997 Apr 10;386(6625):627-30.

   Insert

   λCI-β-flap fusion
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac UV5

   Available since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFREE_Cm

   Plasmid

   #92052

   Purpose

   Allows inducible expression of gRNAs and Cas9 for plasmid curing.

   Depositor

   Morten Norholm

   Article

   Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

   Inserts

   Cas9
   gRNA array
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   pRhamBAD and ptet

   Available since

   Aug. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKIKOlacZCm

   Plasmid

   #46764

   Purpose

   E.coli genomic integration at lacZ

   Depositor

   Lars Nielsen, Claudia Vickers

   Article

   Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

   Type

   Empty backbone

   Use

   E.coli genomic integration

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUC18-mini-Tn7T-Gm-GW

   Plasmid

   #64961

   Purpose

   mini-Tn7 Gateway destination vector

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Methods. 2005 Jun;2(6):443-8.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 2, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pBad-Xis/Int Reset Flipper

   Plasmid

   #38207

   Depositor

   Drew Endy

   Article

   Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

   Inserts

   Bxb1-Excisionase
   Bxb1 Integrase
   

   Use

   Synthetic Biology; Biobrick cloning vector

   Tags

   Expression

   Mutation

   Promoter

   pBAD

   Available since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGL0_7 [pSa ORI]

   Plasmid

   #198923

   Purpose

   Level 0 part

   Depositor

   Nili Ostrov, Cultivarium Tools, Charlie Gilbert

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   pSa ORI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJH643

    Plasmid

    #172168

    Purpose

    Reporter plasmid for pylBCD variant in pBAD backbone

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Para sfGFP.3TAG; Plpp pylS; PproK pylT; AraC; Cam
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    sfGFP(N39*, N135*, Y151*)

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBbB5c-GFP

    Plasmid

    #35353

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available since

    Feb. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGP-Tn7-Pcym

    Plasmid

    #225280

    Purpose

    Mini-Tn7 pGP-Tn7-Cm vector with inducible Pcym cumate promoter for the inducible/controlled expression of target genes inserted into the attTn7 chromosomal site.

    Depositor

    Mark Schembri

    Article

    Chang et al Appl Environ Microbiol. 2024 Oct 23;90(1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pcym cumate (p-isopropylbenzoate)-inducible promo…

    Available since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUdO1c

    Plasmid

    #210230

    Purpose

    high-copy plasmid for bacterial expression

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDae029 (eMutaT7[TadA-8e])

    Plasmid

    #187620

    Purpose

    Expresses eMutaT7[TadA-8e] for mutagenizing genes downstream from a T7 promoter in e.coli

    Depositor

    Seokhee Kim

    Article

    Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

    Insert

    TadA-8e-T7RNAP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGL0_24 [pBBR1-UP ORI]

    Plasmid

    #198937

    Purpose

    Level 0 part

    Depositor

    Nili Ostrov, Cultivarium Tools, Charlie Gilbert

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    pBBR1-UP ORI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    S100L (C299T)

    Promoter

    Available since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKM446 (Flag-DAS+4))

    Plasmid

    #108321

    Purpose

    Orbit integrating plasmid for C-terminal tagging with degradation tag DAS+4, using hygromycin as a selection marker.

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Insert

    attB-Flag-DAS+4
    

    Use

    Orbit integrating plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFTK094-LM-AMA002.0

    Plasmid

    #171366

    Purpose

    Entry Vector compatible, fungal replicating, shuttle backbone vector from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL0_12 [pAMβ1 ORI]

    Plasmid

    #198926

    Purpose

    Level 0 part

    Depositor

    Nili Ostrov, Cultivarium Tools, Charlie Gilbert

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    pAMβ1 ORI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSBDEST.IP

    Plasmid

    #79472

    Purpose

    Promoterless, Sleeping Beauty-compatible Gateway destination vector, IRES Puromycin

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Type

    Empty backbone

    Use

    Promoterless gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSALECT-EcoBam

    Plasmid

    #59705

    Purpose

    Folding reporter with ampicillin resistance as a readout

    Depositor

    Christopher Anderson

    Article

    Hsiau et al PLoS One. 2015 Mar 19;10(3):e0119927. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge