We narrowed to 17,102 results for: emb

1. pKK-RNAi-nucCHERRYmiR-TEV-FLAG

   Plasmid

   #105813

   Purpose

   Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal FLAG.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   RNAi; Flp-in competent

   Tags

   TEV-FLAG

   Expression

   Mammalian

   Available Since

   March 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. W118-1_hRSK3(RPS6KA2)_K91A_K444A

   Plasmid

   #180385

   Purpose

   lentiviral transduction of human RSK3 (RPS6KA2) gene with point mutations (K91A and K444A) that inactivate kinase activity

   Depositor

   Huda Zoghbi

   Article

   Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

   Insert

   RPS6KA2_K91A/K444A (RPS6KA2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   K91A/K444A

   Promoter

   CMV

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAc5.1B-EGFP-DmeIF4E

   Plasmid

   #79258

   Purpose

   Expresses EGFP-tagged DmeIF4E in S2 cells

   Depositor

   Elisa Izaurralde

   Article

   Zekri et al EMBO J. 2013 Apr 3;32(7):1052-65. doi: 1

   Insert

   eIF4E (eIF4E1 Fly)
   

   Use

   Schneider 2 cell expression

   Tags

   EGFP

   Promoter

   Ac5 promoter

   Available Since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. NET23 pEGFP-N2 (1174)

   Plasmid

   #62037

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   NET23 (STING1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

5. STT3A pmRFP (663)

   Plasmid

   #61997

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   STT3A (STT3A Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pKK-RNAi-nucCHERRYmiR-TEV-EGFP

   Plasmid

   #105814

   Purpose

   Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal EGFP.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   RNAi; Flp-in competent

   Tags

   TEV-EGFP

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTH752-CEN-RLuc/min103maxCFLuc

   Plasmid

   #38218

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase
   Renilla luciferase
   

   Expression

   Yeast

   Mutation

   The first 103 codons of the original FLuc gene we…

   Promoter

   ADH1 and TDH3 (=GPD)

   Available Since

   Oct. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTH751-CEN-RLuc/min53maxCFLuc

   Plasmid

   #38217

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase
   Renilla luciferase
   

   Expression

   Yeast

   Mutation

   The first 53 codons of the original FLuc gene wer…

   Promoter

   ADH1 and TDH3 (=GPD)

   Available Since

   Oct. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCV-BRAF-V600E-IRES-mCherry

   Plasmid

   #221026

   Purpose

   Fluorescent reporter for expressing a segment of BRAF-V600E CDS

   Depositor

   Mohamed Fareh

   Article

   Shembrey et al Sci Adv. 2024 Dec 20;10(51):eadl0731. do

   Insert

   BRAF V600E (BRAF Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Aug. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHLsec-dasTMPRSS2

    Plasmid

    #207850

    Purpose

    Expresses directed activation strategy TMPRSS2 in mammalian cells, such as HEK293F- or CHO-cells

    Depositor

    Ingrid De Meester

    Article

    Verhulst et al J Extracell Vesicles. 2025 Mar;14(3):e70

    Insert

    transmembrane serine protease 2 (TMPRSS2 Human)
    

    Tags

    AviTag, 8xHIS tag

    Expression

    Mammalian

    Mutation

    deleted amino acid 1-108, S251D R252D Q253D S254D…

    Promoter

    CAG

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiCRISPR V2 Neo PTEN sgRNA5

    Plasmid

    #233246

    Purpose

    Knock out of murine PTEN

    Depositor

    David Bryant

    Article

    Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

    Insert

    PTEN (Pten Mouse)
    

    Use

    Lentiviral

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLentiCRISPR V2 Neo sgITGA5_5

    Plasmid

    #233253

    Purpose

    Knock out of murine ITGA5

    Depositor

    David Bryant

    Article

    Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

    Insert

    ITGA5 (Itga5 Mouse)
    

    Use

    Lentiviral

    Available Since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLentiCRISPR V2 Neo sgITGA5_4

    Plasmid

    #233252

    Purpose

    Knock out of murine ITGA5

    Depositor

    David Bryant

    Article

    Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

    Insert

    ITGA5 (Itga5 Mouse)
    

    Use

    Lentiviral

    Available Since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiCRISPR V2 Neo sgITGA5_1

    Plasmid

    #233251

    Purpose

    Knock out of murine ITGA5

    Depositor

    David Bryant

    Article

    Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

    Insert

    ITGA5 (Itga5 Mouse)
    

    Use

    Lentiviral

    Available Since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLentiCRISPR V2 Neo sgAGAP1_3

    Plasmid

    #233248

    Purpose

    Knock out of murine AGAP1

    Depositor

    David Bryant

    Article

    Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

    Insert

    AGAP1 (Agap1 Mouse)
    

    Use

    Lentiviral

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiCRISPR V2 Neo PTEN sgRNA2

    Plasmid

    #233245

    Purpose

    Knock out of murine PTEN

    Depositor

    David Bryant

    Article

    Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

    Insert

    PTEN (Pten Mouse)
    

    Use

    Lentiviral

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLentiCRISPR V2 Neo sgAGAP1_2

    Plasmid

    #233247

    Purpose

    Knock out of murine AGAP1

    Depositor

    David Bryant

    Article

    Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

    Insert

    AGAP1 (Agap1 Mouse)
    

    Use

    Lentiviral

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pME-Actin-Vhh-sfGFP-P2A-1xHA-iRFP-caax (JDW 1216)

    Plasmid

    #224496

    Purpose

    Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and iRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Actin-Vhh-sfGFP-P2A-HA-iRFP-caax
    

    Use

    Gateway cloning

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. PABPC1_544-636_pGEX6P1

    Plasmid

    #209789

    Purpose

    Bacterial expression of PABPC1 Mlle domain

    Depositor

    Kalle Gehring

    Article

    Kozlov et al EMBO J. 2004 Jan 28;23(2):272-81. doi: 1

    Insert

    Mlle domain of PABPC1 (PABPC1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 544-636 only

    Promoter

    tac

    Available Since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. eIF3k sgRNA-2 PX459 plasmid

    Plasmid

    #192256

    Purpose

    expressing sgRNA-2 targeting eIF3k locus closed to stop coden

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-2 targeting eIF3k locus closed to stop coden (EIF3K Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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