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PX458 eSp(1.1) V3

Plasmid

#226963

Purpose

CBh-eSpCas9(1.1)-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

Depositor

Paul Thomas

Article

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-RiboL1-jGCaMP8f

Plasmid

#169249

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8f protein.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8f

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

hSyn

Available Since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pStreptagII 3C SMO

Plasmid

#234995

Purpose

Expression of a Strep-tag II labeled on its N-terminus receptor, with the Smoothened (Smo) receptor

Depositor

Monica Gonzalez-Magaldi, Daniel Leahy

Article

Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

Insert

SMO (SMO Human)

Tags

Streptag-II, HRV 3C site

Expression

Mammalian

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV5 WT KSR1

Plasmid

#25970

Depositor

Rob Lewis

Article

Joneson et al J Biol Chem. 1998 Mar 27. 273(13):7743-8

Insert

Kinase suppressor of Ras 1 (Ksr1 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only

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AAV-Syn-NS1

Plasmid

#175276

Purpose

AAV vector mediating bicistronic expression of NS1 gene of YFV-17D and dTomato with NLS

Depositor

Wei Xu

Article

Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

Insert

Nonstructural protein 1 (NS1) of YFV-17D; NLS-dTomato (POLY Yellow fever virus strain 17D)

Use

AAV

Tags

NLS-dTomato (P2A cleavage)

Promoter

Synapsin

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-rtTA2-TRE-SspB-mScarlet-I-MYPT169

Plasmid

#178528

Purpose

Expresses rtTA and SspB-mScarlet-I-MYPT (1-169 aa) in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

rtTA, SspB-mScarlet-I-MYPT (1-169aa)

Expression

Mammalian

Promoter

CAG, Tet responsive element

Available Since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pET30a-His6-ABP-LOXCAT

Plasmid

#139971

Purpose

Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2. Contains a albumin binding peptide (ABP).

Depositor

Vamsi Mootha

Article

Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

Insert

A fusion of lactate oxidase from A. viridans and catalase from E. coli

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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tetO-FHL2

Plasmid

#170686

Purpose

doxycycline-inducible overexpression of FHL2

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

FHL2 (FHL2 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-GRET

Plasmid

#158222

Purpose

Control lentiviral plasmid encoding GFP-Nluc BRET reporter from Schaub et al., Cancer Research, 2015.

Depositor

Charles P. Lai

Article

Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014

Insert

GFP-Nluc

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pLL5.0 E-cadherin shRNA/E-cadherin GFP-IRES-HA-H-Ras V12

Plasmid

#101285

Purpose

Lentiviral expression ,was generatedby recombining bothIRES an HA-tagged H-Ras V12 into SbfI site of pLL5.0 Ecadherin shRNA/mEcad-GFP

Depositor

Alpha Yap

Article

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Insert

E-Cadherin-GFP-H-Ras

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

H-Ras V12

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-Zdk1-EB1C

Plasmid

#107696

Purpose

C-terminal half of π-EB1, EGFP-tagged, dissociates from microtubule ends in blue light

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE1 (MAPRE1 Human)

Tags

EGFP and Zdk1

Expression

Mammalian

Mutation

EB1 aa 186-268

Promoter

CMV

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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FHL-cpmTq2-Calcium-lifetime-sensor

Plasmid

#129628

Purpose

Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

Depositor

Dorus Gadella

Article

van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

Insert

FHL-cpmTq2-Calcium-lifetime-sensor

Tags

6x His - Modified TorA MNNNDLFQASRRRFLAQLG[G to …

Expression

Bacterial and Mammalian

Promoter

CMV,&Rhamnose

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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ACE2-Fc

Plasmid

#164222

Purpose

Expresses soluble , dimeric Fc-His fusion protein for the human ACE2 extracellular domain, along with dTomato reporter

Depositor

Sriram Neelamegham

Article

Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

Insert

ACE2 extracellular domain (ACE2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-TRE-NS1-dTomato

Plasmid

#175278

Purpose

AAV vector mediating inducible bicistronic expression of NS1 gene of YFV-17D and dTomato

Depositor

Wei Xu

Article

Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

Insert

NS1; dTomato (POLY Yellow fever virus strain 17D)

Use

AAV

Tags

dTomato (from bidirectional promoter)

Promoter

bidirectional TRE promoter

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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GLuc-PM-Venus

Plasmid

#164785

Purpose

Gaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent protein

Depositor

Laszlo Hunyady

Article

Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.

Insert

Gaussia luciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Gaussia princeps, Human)

Tags

GLuc and mVenus

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Granulin 4-no linker

Plasmid

#176925

Purpose

express Granulin 4 without linker 5 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 4 (GRN Human)

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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PL-5LTR-GW-A

Plasmid

#138480

Purpose

For expression of ribozyme-flanked sgRNA after the Gateway (GW) LR cloning.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

Gateway attR1/R2 cassette with chloramphenicol resistance and ccdB genes

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pmCherry-Zdk1-EB1C

Plasmid

#107695

Purpose

C-terminal half of π-EB1, mCherry-tagged, dissociates from microtubule ends in blue light

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE1 (MAPRE1 Human)

Tags

Zdk1 and mCherry

Expression

Mammalian

Mutation

EB1 aa 186-268

Promoter

CMV

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-TdT

Plasmid

#126450

Purpose

Expresses human TdT in mammalian cells. (pTBL206)

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

TdT (DNTT Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-hGTB

Plasmid

#26196

Depositor

Edward Callaway

Article

Wall et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Tet transactivator, enhanced green fluorescent protein, TVA, rabies B19 glycoprotein

Use

AAV and Cre/Lox; Adeno-associated virus

Mutation

F2A has 2A cleavage site deleted, producing htTA-…

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

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