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pC0.069

Plasmid

#119661

Purpose

Level 0 part. Terminator from arcA gene from E. coli.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

ECK120026481

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.073

Plasmid

#119660

Purpose

Level 0 part. Terminator from rplJL-rpoBC,rplKAJL-rpoBC,rpoBC gene cluster from E. coli.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

ECK120010869

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.072

Plasmid

#119657

Purpose

Level 0 part. Terminator from hyaABCDEF operon from E. coli.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

ECK120010842

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.077

Plasmid

#119656

Purpose

Level 0 part. Terminator from rpoH gene from E. coli.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

ECK120010841-R

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.064

Plasmid

#119655

Purpose

Level 0 part. Terminator from hupB gene from E. coli.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

ECK120010820

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.070

Plasmid

#119652

Purpose

Level 0 part. Terminator, Bi-directional terminator for yciA/tonA genes from E. coli.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

BBa_B0061

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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LOC5570372 NPFF receptor

Plasmid

#115378

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

LOC5570372 NPFF receptor (LOC5570372 Aedes aegypti)

Expression

Mammalian

Mutation

See depositor comments below

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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LOC5570884 Cardioacceleratory peptide receptor

Plasmid

#115373

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

LOC5570884 Cardioacceleratory peptide receptor (LOC5570884 Aedes aegypti)

Expression

Mammalian

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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LOC5570885 Cardioacceleratory peptide receptor

Plasmid

#115375

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

LOC5570885 Cardioacceleratory peptide receptor (LOC5570885 Aedes aegypti)

Expression

Mammalian

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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LOC5574680 Atrial natriuretic peptide receptor

Plasmid

#115374

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

LOC5574680 Atrial natriuretic peptide receptor (LOC5574680 Aedes aegypti)

Expression

Mammalian

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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P0050::kaiBC S431A T432A NS1

Plasmid

#101837

Purpose

drives expression of kaiBC S431A T432A variant from an rpaA-independent promoter in NS1 locus

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

kaiBC S431A T432A with p0050 promoter

Use

Cyanobacteria cloning vector

Mutation

S431A T432A

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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P0050::kaiBC S431E T432E NS1

Plasmid

#101836

Purpose

drives expression of kaiBC S431E T432E variant from an rpaA-independent promoter in NS1 locus

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

kaiBC S431E T432E with p0050 promoter

Use

Cyanobacteria cloning vector

Mutation

S431E T432E

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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nucHCB1

Plasmid

#97424

Purpose

Nuclear Huntingtin Chromobody 1: "Happ1" intrabody recognizing amino acids 41–81 of huntingtin, fused to eYFP and nuclear localization signal.

Depositor

Ray Truant

Article

Maiuri et al Hum Mol Genet. 2016 Dec 25. pii: ddw395.

Insert

nucHCB1

Tags

NLS and YFP

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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PHBA03

Plasmid

#83537

Purpose

Constitutive TEF1 promoter mediated expression of the UBiC, ARO4*, and TKL1 genes

Depositor

Claudia Vickers

Article

Williams et al Metab Eng Commun. 2016 May 11;3:142-152.

Insert

pTEF1-UBiC-CYC1t-pTEF1-ARO4*-CYC1t-pTEF1-TKL1-CYC1t

Expression

Yeast

Promoter

pTEF1

Available Since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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const #7-RFP

Plasmid

#81081

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23109

Available Since

Aug. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pconst #6-RFP

Plasmid

#81080

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23117

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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UAS-Xbp1spliced

Plasmid

#80628

Purpose

expression of Xbp1 RB isoform in Drosophila

Depositor

Pedro Domingos, Herman Steller

Article

Ryoo et al EMBO J. 2007 Jan 10;26(1):242-52. Epub 2

Insert

Xbp1 (Xbp1 Fly)

Expression

Insect

Mutation

RB isoform

Promoter

hsp70

Available Since

July 28, 2016

Availability

Academic Institutions and Nonprofits only
GST-PRMT9 deltaTPR mutant

Plasmid

#67614

Purpose

bacterial expression of GST-PRMT9 missing residues 21-139

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Tags

GST

Expression

Bacterial

Mutation

truncation mutant missing aa 21-139

Promoter

tac

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
GST-PRMT9 deltaN (aa 21-350)

Plasmid

#67611

Purpose

bacterial expression of GST-PRMT9 missing residues 21-350

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Tags

GST

Expression

Bacterial

Mutation

truncation mutant missing aa 21-350

Promoter

tac

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
Kif17 1-490 motor p201

Plasmid

#44706

Depositor

Geri Kreitzer

Article

Acharya et al J Biol Chem. 2013 Sep 26.

Insert

Kif17 (KIF17 Human)

Use

Gateway donor vector

Mutation

amino acids 1-490

Available Since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only
pBGSA FPR1 H4 (haplotype 4)

Plasmid

#66718

Purpose

Constitutive expression of FPR1 in mammalian cells

Depositor

Heini Miettinen

Article

Gripentrog et al Immunogenetics. 2008 Feb;60(2):83-93. do

Insert

FPR1 (haplotype 4) (FPR1 Human)

Expression

Mammalian

Promoter

SR-alpha

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
p3.2mar-Luc

Plasmid

#63696

Purpose

3.2 kb marine armor plate enhancer driving Luciferase expression

Depositor

David Kingsley

Article

O'Brown et al Elife. 2015 Jan 28;4:e05290. doi: 10.755

Insert

3.2 kb marine stickleback armor plate enhancer

Tags

Luciferase

Expression

Mammalian

Promoter

TATA box

Available Since

April 7, 2015

Availability

Academic Institutions and Nonprofits only

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pLL3.7 Lmo3.5 shRNA

Plasmid

#59295

Purpose

shRNA to mouse Lmo3

Depositor

Ulrike Heberlein

Article

Lasek et al Alcohol Clin Exp Res. 2011 Sep;35(9):160

Insert

Lmo3.5 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

mouse U6

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pLL3.7 Lmo3.8 shRNA

Plasmid

#59294

Purpose

shRNA to mouse Lmo3

Depositor

Ulrike Heberlein

Article

Lasek et al Alcohol Clin Exp Res. 2011 Sep;35(9):160

Insert

Lmo3.8 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

mouse U6

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
psicheck-2-Phb

Plasmid

#48162

Purpose

contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of Phb (Phb Mouse)

Use

Luciferase

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
psicheck-2-Slc25a15 mod

Plasmid

#48161

Purpose

contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading from element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of Slc25a15

Use

Luciferase

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
psicheck-2-Mrpl1

Plasmid

#48166

Purpose

contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of MRPL11 (Mrpl11 Mouse)

Use

Luciferase

Available Since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only
psicheck-2-Slc25a15

Plasmid

#48160

Purpose

contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of Slc25a15

Use

Luciferase

Available Since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only
pGEX4T3-GST-Tev

Plasmid

#177142

Purpose

Bacterial vector for expression of an N-terminal GST fusion of the Tev protease

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

Tev

Tags

GST

Expression

Bacterial

Promoter

Tac

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pJRH-1346 U6-B2M sgRNA Gag-pol v2

Plasmid

#201917

Purpose

Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Inserts

Gag-pol
B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)

Expression

Mammalian

Promoter

CAG and Human U6

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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