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We narrowed to 23,359 results for: Spr

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CROPseq-multi-v2-mTagBFP2-NLS-BsmBI_entry (pRW1506)

Plasmid

#225754

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with nuclear-localized mTagBFP2-NLS; v2 is compatible with T7 in vitro transcription detection

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEA186

Plasmid

#187874

Purpose

Empty pVS1-based INTEGRATE vector with mCherry cargo

Depositor

Kan Wang

Article

Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ133A

Plasmid

#69275

Purpose

Golden Gate entry vector; 3rd gRNA under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pKL2310

Plasmid

#187875

Purpose

Empty pVS1-based INTEGRATE vector with loxP cargo

Depositor

Kan Wang

Article

Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pHelper_ShCAST

Plasmid

#127922

Purpose

Expresses ShCAST and a crRNA targeting PSP1

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

ShTnsB, ShTnsC, ShTniQ, ShCas12k

Use

Tags

Expression

Mutation

Promoter

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pET-Himar-dCas9

Plasmid

#137079

Purpose

Himar-dCas9 gene with C-terminal 6x-His tag for protein purification

Depositor

Harris Wang

Article

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar-dCas9

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ134A

Plasmid

#69276

Purpose

Golden Gate entry vector; 4th gRNA under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-MCP-3XBFPnls

Plasmid

#75383

Purpose

MCP-3XBFPnls

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

MCP

Use

Lentiviral

Tags

3XBFP

Expression

Mammalian

Mutation

Promoter

CMV-TO

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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LRG2.1-TagBFP2

Plasmid

#124773

Purpose

Lentiviral plasmid to co-express a guide RNA and TagBFP2

Depositor

Jason Sheltzer

Article

Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pIF4

Plasmid

#196069

Purpose

Expresses dCas9-Mxi1 with MoRP27 promoter and express gRNA with MoU6 promtoer in M. oryzae

Depositor

Kabin Xie

Article

Zhang et al mSphere. 2023 Feb 21;8(1):e0059422. doi:

Insert

dCas9-Mxi1

Use

CRISPR

Tags

Expression

Mutation

Promoter

MoRP27 promoter

Available since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

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p(U6a-BsaI-gRNA)

Plasmid

#65955

Purpose

Tribolium U6a promoter with BsaI cloning site to insert guide RNA sequence

Depositor

Michalis Averof

Article

Gilles et al Development. 2015 Jul 9. pii: dev.125054

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

U6

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pJMP2834

Plasmid

#160673

Purpose

Mobile CRISPRi sfGFP 'test', gmc6 sgRNA

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

gmc6 (gfp) sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHelper_AcCAST

Plasmid

#127923

Purpose

Expresses AcCAST and a crRNA targeting PSP1

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

AcTnsB, AcTnsC, AcTniQ, AcCas12k

Use

Tags

Expression

Mutation

Promoter

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pJW1219

Plasmid

#61250

Purpose

CRISPR/Cas9 plasmid containing sgRNA(F+E); for use in C. elegans

Depositor

Jordan Ward

Article

Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

Insert

sgRNA(F+E)

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6 promoter

Available since

Dec. 17, 2014

Availability

Academic Institutions and Nonprofits only

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sgBbsI (p2Tol-U6-2xBbsI-sgRNA-HygR)

Plasmid

#71485

Purpose

Base plasmid for sgRNA cloning with Hygromycin resistance and Tol2 transposon arms

Depositor

Richard Sherwood

Article

Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

U6

Available since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp28

Plasmid

#73850

Purpose

pBT264-U6p-{2xBbsI}-sgRNA scaffold-{MfeI}

Depositor

Timothy Lu

Article

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ131C

Plasmid

#69284

Purpose

Golden Gate entry vector; 1st gRNA under OsU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ132C

Plasmid

#69285

Purpose

Golden Gate entry vector; 1st gRNA under OsU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH376

Plasmid

#86842

Purpose

mamalian expression of anti-CRISPR protein AcrIIA4

Depositor

Joseph Bondy-Denomy

Article

Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

Insert

acrIIA4

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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pJW1285

Plasmid

#61252

Purpose

CRISPR/Cas9 plasmid with sgRNA(F+E) targeting pha-1; for co-conversion in C. elegans

Depositor

Jordan Ward

Article

Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

Insert

sgRNA(F+E) targeting pha-1

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

Dec. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pOC1 cloning template vector

Plasmid

#183420

Purpose

CreON knock-in vector

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pOC2 cloning template vector

Plasmid

#183421

Purpose

CreOFF knock-in vector

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pIF3

Plasmid

#196068

Purpose

Expresses dCas9-Mxi1 with MoRP27 promoter and express gRNA with MoU3 promtoer in M. oryzae

Depositor

Kabin Xie

Article

Zhang et al mSphere. 2023 Feb 21;8(1):e0059422. doi:

Insert

dCas9-Mxi1

Use

CRISPR

Tags

Expression

Mutation

Promoter

MoRP27 promoter

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Construct 12 - hUBCp_Cas9_3xNLS_p2a_puroR

Plasmid

#81251

Purpose

Lentiviral construct for building stable cell lines expressing Cas9 via puromycin selection

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

Cas9-3xNLS

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ135A

Plasmid

#69277

Purpose

Golden Gate entry vector; 5th gRNA under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pJMP2832

Plasmid

#160672

Purpose

Mobile CRISPRi sfGFP 'test', gmc6 sgRNA

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

gmc6 (gfp) sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ136A

Plasmid

#69278

Purpose

Golden Gate entry vector; 6th gRNA under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC1812_pCR8-gCasRx-CUG (control gRNA)

Plasmid

#118645

Purpose

Transient transfection; Expressed Control (CUG repeat) CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gCasRx-CUG

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ133C

Plasmid

#69286

Purpose

Golden Gate entry vector; 1st gRNA under OsU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
p(U6b-BsaI-gRNA)

Plasmid

#65956

Purpose

Tribolium U6b promoter with BsaI cloning site to insert guide RNA sequence

Depositor

Michalis Averof

Article

Gilles et al Development. 2015 Jul 9. pii: dev.125054

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

U6

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
PP7 guide

Plasmid

#86168

Purpose

Expresses S. Pyogenes guide with PP7 aptamer in mammalian cells

Depositor

Amit Choudhary

Article

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

PP7 guide

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMLS291

Plasmid

#73724

Purpose

SapTrap mCherry + Cbr-unc-119 donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

mCherry + Cbr-unc-119

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMLS252

Plasmid

#73720

Purpose

SapTrap GFP + Cbr-unc-119 donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

GFP + Cbr-unc-119

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ132D

Plasmid

#69288

Purpose

Golden Gate entry vector; 1st gRNA under OsU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMLS286

Plasmid

#73723

Purpose

Saptrap tagRFP + Cbr-unc-119 donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

tagRFP + Cbr-unc-119

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBRC1

Plasmid

#183064

Purpose

Base editing plasmid for Pseudomonas chlororaphis

Depositor

Xuehong Zhang

Article

Yue et al Microb Biotechnol. 2022 Sep;15(9):2324-2

Insert

eSpCas9ppD10A

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ131D

Plasmid

#69287

Purpose

Golden Gate entry vector; 1st gRNA under OsU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Construct 33 - NFKBRp_Cas9_3xNLS_p2a-puroR

Plasmid

#81255

Purpose

Lentiviral construct for building stable cell lines expressing Cas9 in the presence of TNFa via puromycin selection

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

Cas9-3xNLS

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJMP2774

Plasmid

#160667

Purpose

Mobile CRISPRi sfGFP 'test', gmc6 sgRNA

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

gmc6 (gfp) sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJMP2836

Plasmid

#160674

Purpose

Mobile CRISPRi sfGFP 'test', gmc6 sgRNA

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

gmc6 (gfp) sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
iD20 LoxStopLox-Zim3-dCas9-mApple-hygro

Plasmid

#204720

Purpose

Cre-inducible CRISPRi by Zim3-dCas9

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Zim3-dCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMZ222

Plasmid

#52223

Purpose

Constitutive expression of humanized Cas9

Depositor

Mikel Zaratiegui

Article

Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

Insert

Cas9

Use

Tags

Expression

Yeast

Mutation

silent mutation in the original CspCI site

Promoter

adh1

Available since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pUCSh5-2300

Plasmid

#171788

Purpose

Encodes sgRNA target 2300 and Gentamicin resistance transposon.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

Sh-CAST sgRNA 2300

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Adrb1

Plasmid

#184294

Purpose

A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Adrb1

Depositor

Bernardo Sabatini

Insert

sgRNA-Adrb1

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR8/GW:U6

Plasmid

#85981

Purpose

Golden Gate Level 0 cassette encoding the U6 promoter

Depositor

Thomas Mock

Article

Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

Insert

T. pseudonana U6 promoter

Use

CRISPR; Diatom (t. pseudonana)

Tags

Expression

Mutation

Promoter

none

Available since

Jan. 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW209

Plasmid

#113188

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEC-K-CBP_CjeCas9

Plasmid

#83043

Purpose

Expresses Campylobacter jejuni Cas9 (Cje Cas9).

Depositor

Jennifer Doudna

Article

Ma et al Mol Cell. 2015 Nov 5;60(3):398-407. doi:

Insert

CjeCas9

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only
pCD175

Plasmid

#113316

Purpose

dCas9, MCP-AsiA, rpoD_F563Y

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9, MCP-T4.AsiA, rpoD_F563Y

Use

CRISPR

Tags

Expression

Bacterial

Mutation

F563Y

Promoter

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMLS271

Plasmid

#73722

Purpose

SapTrap SNAPtag + Cbr-unc-119 donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Snaptag + Cbr-unc-119

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ138A

Plasmid

#69280

Purpose

Golden Gate entry vector; 8th gRNA under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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