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We narrowed to 11,726 results for: pcDNA

Showing: 8001 - 8050 of 11726 results

  6. Rescue myc-PCDHGC5 mRNA

    Plasmid
    #122229
    Purpose
    Rescue mRNA for Protocadherin gamma C5 knock down with sh1 shRNA (plasmid 122227). With five silent mutations at sh1 shRNA target site.
    Depositor
    Angel de Blas
    Article
    Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.
    Insert
    PCDHGC5 (Pcdhgc5 Rat)
    Use
    Tags
    9E10 cMyc epitope (EQKLISEEDL) was inserted betwe…
    Expression
    Mammalian
    Mutation
    Silent mutations are in five consecutive codons …
    Promoter
    Available since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    122229-plasmid-map-sequence-id-237759

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  7. CCR6-DuET

    Plasmid
    #213202
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCR6 (CCR6 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213202-plasmid-map-sequence-id-430765

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  10. Venus2-Y705F/S727A-STAT3

    Plasmid
    #123177
    Purpose
    Expresses Y705F/S727A STAT3 double mutant fused to the 158-238 fragment of Venus in mammalian cells
    Depositor
    Federico Herrera
    Article
    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.
    Insert
    STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
    Use
    Tags
    Venus2 (aa 158-238) for bimolecular fluorescence …
    Expression
    Mammalian
    Mutation
    Double Y705F and S727A substitutions
    Promoter
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123177-plasmid-map-sequence-id-238660

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  11. Venus1-Y705F/S727A-STAT3

    Plasmid
    #123176
    Purpose
    Expresses Y705F/S727A STAT3 double mutant fused to the 1-157 fragment of Venus in mammalian cells
    Depositor
    Federico Herrera
    Article
    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.
    Insert
    STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
    Use
    Tags
    Venus1 (aa 1-158) for bimolecular fluorescence co…
    Expression
    Mammalian
    Mutation
    Double Y705F and S727A substitutions
    Promoter
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123176-plasmid-map-sequence-id-238648

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  12. Venus2-DeltaCT-STAT3

    Plasmid
    #123179
    Purpose
    Expresses a STAT3 deletion mutant (without the C-terminus from SH2 domain) fused to the 158-238 fragment of Venus in mammalian cells
    Depositor
    Federico Herrera
    Article
    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.
    Insert
    STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
    Use
    Tags
    Venus2 (aa 158-238) for bimolecular fluorescence …
    Expression
    Mammalian
    Mutation
    Deletion of the SH2 and TAD domains (C-terminus, …
    Promoter
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123179-plasmid-map-sequence-id-238651

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  15. Venus1-DeltaCT-STAT3

    Plasmid
    #123178
    Purpose
    Expresses a STAT3 deletion mutant (without the C-terminus from SH2 domain) fused to the 1-157 fragment of Venus in mammalian cells
    Depositor
    Federico Herrera
    Article
    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.
    Insert
    STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
    Use
    Tags
    Venus1 (aa 1-158) for bimolecular fluorescence co…
    Expression
    Mammalian
    Mutation
    Deletion of the SH2 and TAD domains (C-terminus, …
    Promoter
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123178-plasmid-map-sequence-id-238649

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  17. VC-Y705F+K49R-STAT3

    Plasmid
    #203721
    Purpose
    Expresses the PTM-resistant, double mutant Y705F+K49R - STAT3 fused to the 158-238 fragment of Venus in mammalian cells
    Depositor
    Federico Herrera
    Article
    Diallo et al Cell Oncol (Dordr). 2023 Dec 27. doi: 10
    Insert
    STAT3 tagged with VC (aa 158-238) (STAT3 Human)
    Use
    Tags
    VC (aa 158-238) for bimolecular fluorescence comp…
    Expression
    Mammalian
    Mutation
    Y705F+K49R substitutions
    Promoter
    CMV
    Available since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    203721-plasmid-map-sequence-id-401591

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  18. VC-Y705F+K685R-STAT3

    Plasmid
    #203722
    Purpose
    Expresses the PTM-resistant, double mutant Y705F+K685R - STAT3 fused to the 158-238 fragment of Venus in mammalian cells
    Depositor
    Federico Herrera
    Article
    Diallo et al Cell Oncol (Dordr). 2023 Dec 27. doi: 10
    Insert
    STAT3 tagged with VC (aa 158-238) (STAT3 Human)
    Use
    Tags
    VC (aa 158-238) for bimolecular fluorescence comp…
    Expression
    Mammalian
    Mutation
    Y705F+K685R substitutions
    Promoter
    CMV
    Available since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    203722-plasmid-map-sequence-id-401596

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  19. pcmCherry 3.1(-)

    Plasmid
    #62803
    Purpose
    Modified pcDNA3.1(-), with Neomycin resistance cassette replaced by mCherry
    Depositor
    Michal Minczuk
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    62803-plasmid-map-sequence-id-103515

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  20. pUB2.1

    Plasmid
    #40728
    Purpose
    Modified pcDNA3.1 vector for ligation independent cloning, including a ccdB cassette for eliminating no-insert background
    Depositor
    Thomas Hughes
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 29, 2012
    Availability
    Academic Institutions and Nonprofits only
    40728-plasmid-map-sequence-id-128586

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  21. pCMV6 SMCR8 HA

    Plasmid
    #74158
    Purpose
    Express SMCR8 HA tagged in mammalian cells
    Depositor
    Nicolas Charlet-Berguerand
    Insert
    SMCR8 (SMCR8 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    74158-plasmid-map-sequence-id-139169

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  22. pCMV6 WDR41 HA

    Plasmid
    #74159
    Purpose
    Express WDR41 HA tagged in mammalian cells
    Depositor
    Nicolas Charlet-Berguerand
    Insert
    WDR41 (WDR41 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    74159-plasmid-map-sequence-id-142470

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  25. FVIII H2

    Plasmid
    #46773
    Purpose
    pcDNA4.full length FVIII D1241E
    Depositor
    Robert Peters
    Insert
    full length FVIII D1241E (F8 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D1241E
    Promoter
    CMV
    Available since
    Nov. 26, 2013
    Availability
    Academic Institutions and Nonprofits only
    46773-plasmid-map-sequence-id-66265

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  26. PRPF4.001.522.K45A.pc4

    Plasmid
    #137648
    Purpose
    Expresses nuclear localization signal (NLS), C-terminal myc-(His)6 fusion of human gene or portion of gene in human cell culture lines. pcDNA4.1-based vector.
    Depositor
    Tara Davis
    Insert
    PRPF4 (PRPF4 Human)
    Use
    Tags
    NLS and myc-(His)6
    Expression
    Mammalian
    Mutation
    001-522-K45A-pc4
    Promoter
    Available since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    137648-plasmid-map-sequence-id-381373

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  27. PRPF4.001.522.Y32A.pc4

    Plasmid
    #137646
    Purpose
    Expresses nuclear localization signal (NLS), C-terminal myc-(His)6 fusion of human gene or portion of gene in human cell culture lines. pcDNA4.1-based vector.
    Depositor
    Tara Davis
    Insert
    PRPF4 (PRPF4 Human)
    Use
    Tags
    NLS and myc-(His)6
    Expression
    Mammalian
    Mutation
    001-522-Y32A-pc4
    Promoter
    Available since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    137646-plasmid-map-sequence-id-381367

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  28. BDD FVIII H4

    Plasmid
    #46774
    Purpose
    pcDNA4.BDD FVIII.H4(R484H)
    Depositor
    Robert Peters
    Insert
    B-domain deleted FVIII H4 (R484H) (F8 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R484H
    Promoter
    CMV
    Available since
    Nov. 26, 2013
    Availability
    Academic Institutions and Nonprofits only
    46774-plasmid-map-sequence-id-66260

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  29. BDD FVIII H3

    Plasmid
    #46775
    Purpose
    pcDNA4.BDD FVIII.H3(M2238V)
    Depositor
    Robert Peters
    Insert
    B-domain deleted FVIII H3 protein (M2238V) (F8 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    M2238V
    Promoter
    CMV
    Available since
    Aug. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    46775-plasmid-map-sequence-id-66268

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  30. pEZYmyc-His

    Plasmid
    #18701
    Purpose
    pcDNA3.1(-)/Myc-His B converted Gateway Destination vector for expressing C-terminal myc-His tagged fusion proteins in mammalian cells
    Depositor
    Yu-Zhu Zhang
    Article
    Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.
    Type
    Empty backbone
    Use
    Tags
    Myc-His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 26, 2008
    Availability
    Academic Institutions and Nonprofits only
    18701-plasmid-map-sequence-id-181026

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  32. pET3C-ARL16(CO)-His6

    Plasmid
    #216485
    Purpose
    Used for expression of human ARL16, short form, in bacteria. The open reading frame has been codon optimized for bacterial expression and contains a glycine rich linker and His6 tag at the C-terminus.
    Depositor
    Richard Kahn
    Insert
    ADP-ribosylation factor-like 16 (ARL16 Human)
    Use
    Tags
    includes GSGSGSHHHHHH (His6) tag at C-terminus
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    216485-plasmid-map-sequence-id-426900

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  48. dCas9 plasmid

    Plasmid
    #100091
    Purpose
    dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Article
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    3XFlag-NLS-dCas9-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100091-plasmid-map-sequence-id-197565

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Showing: 8001 - 8050 of 11726 results