We narrowed to 13,107 results for: CRISPR-Cas9

1. SpCas9-TadCBEd-ivt-template

   Plasmid

   #193843

   Purpose

   Template for in vitro transcription of TadCBEd (with SpCas9)

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadCBEd with SpCas9
   

   Use

   In vitro transcription template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. (517) SB KRAB-SadCas9 U6-gStop

   Plasmid

   #163023

   Purpose

   Sleeping Beauty TET-On expression of CRISPRi with gSTOP gRNA

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Mammalian codon-optimized SadCas9
   

   Use

   Transposon

   Tags

   KRAB and myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-On

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9-2A-Puro-TBK1-gRNA (PX459)

   Plasmid

   #221550

   Purpose

   Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   gRNA targeting human TBK1 (TBK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   u6

   Available since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGinB-8X(GGS)-dCas9-FLAG-NLS

   Plasmid

   #81205

   Purpose

   CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus

   Depositor

   David Liu

   Article

   Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

   Insert

   pGinB-8X(GGS)-dCas9-FLAG-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGinB-8X(GGS)-dCas9-FLAG-NLS_SpecR

   Plasmid

   #81206

   Purpose

   CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus. Spec resistance

   Depositor

   David Liu

   Article

   Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

   Insert

   pGinB-8X(GGS)-dCas9-FLAG-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJT340 Lenti pEF-dCas9-3XFLAG-KRAB[WSR7EEE] + MGA1+2 + RYBP-T2A-tagBFP-P2A-Blast

   Plasmid

   #187344

   Purpose

   dCas9 effector fusion for manipulating transcription

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   dCas9-3XFLAG-KRAB[WSR7EEE] + MGA1+2 + RYBP-T2A-tagBFP-P2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   KRAB WSR7EEE

   Promoter

   pEF1a

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJT242 Lenti pEF-dCas9-3XFLAG-ZNF10 KRAB[WSR7EEE] HighExpr-T2A-tagBFP-P2A-Blast

   Plasmid

   #187327

   Purpose

   dCas9 effector fusion for manipulating transcription

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   dCas9-3XFLAG-ZNF10 KRAB[WSR7EEE] HighExpr-T2A-tagBFP-P2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   KRAB WSR7EEE

   Promoter

   pEF1a

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJT341 Lenti pEF-dCas9-3XFLAG-KRAB[WSR7EEE] + RYBP + MGA1+2-T2A-tagBFP-P2A-Blast

   Plasmid

   #195540

   Purpose

   dCas9 effector fusion for manipulating transcription

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti pEF-dCas9-3XFLAG-KRAB[WSR7EEE] + RYBP + MGA1+2-T2A-tagBFP-P2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   pEF1a

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCB-CRISPRi

   Plasmid

   #221136

   Purpose

   Coxiella burnetii CRISPRi plasmid without sgRNA construct. Expresses 3xF-dCas9.

   Depositor

   Craig Roy

   Article

   Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

   Insert

   3xF-dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   cbu1169 promoter

   Available since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. L40C-CRISPR.EFS.mNeon

    Plasmid

    #69146

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), mNeon coexpression, EFS Promoter driven

    Depositor

    Dirk Heckl

    Article

    Reimer et al Haematologica. 2017 Jun 1. pii: haematol

    Inserts

    SpCas9
    Sp sgRNA scaffold
    EFS (EEF1A1 Human)
    P2A-mNeonGreen
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLQ2812 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9

    Plasmid

    #84240

    Purpose

    Expresses GA-inducible VPR-Sp dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    GAI-tagBFP-Sp dCas9
    GID1-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and PGK

    Available since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiCRISPRv2-7SK-Blast

    Plasmid

    #183620

    Purpose

    lentiCRISPRv2 derivative with modified 7SK promoter driving sgRNA and encoding Blasticidin resistance. Compatible with inserts designed for lentiCRISPRv2.

    Depositor

    Bill Sugden

    Article

    Hutcheson et al ACS Synth Biol. 2022 Jun 29. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Modified 7SK

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-T4DNApol (JO638)

    Plasmid

    #208956

    Purpose

    A variant CE1 construct with T4 DNA polymerase (-exo, activity enhanced), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-T4pol-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A), T4Pol(-exo;D189A/E191A/L412M)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. HUHe variant click editor (CE1) - pCMV-T7-DCV-nCas9-EcKlenow (JO608)

    Plasmid

    #208946

    Purpose

    A variant CE1 construct with DCV HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    DCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. HUHe variant click editor (CE1) - pCMV-T7-MSMV-nCas9-EcKlenow (JO661)

    Plasmid

    #208947

    Purpose

    A variant CE1 construct with MSMV HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    MSMV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-ePhi29 (LM2993)

    Plasmid

    #208959

    Purpose

    A variant CE1 construct with ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-ePhi29-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); ePhi29(-exo)(D169A;M8R/V51A/M97T/…

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-Phi29 (JO582)

    Plasmid

    #208954

    Purpose

    A variant CE1 construct with Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-Phi29(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A), Phi29(-exo;D169A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBK2047-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA2)

    Plasmid

    #223167

    Purpose

    2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA2 targeting human/mouse APOE

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB-MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    EF1a

    Available since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. HUHe variant click editor (CE1) - pCMV-T7-TGMV-nCas9-EcKlenow (JO659)

    Plasmid

    #208951

    Purpose

    A variant CE1 construct with TGMV HUH enconuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    TGMV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HUHe variant click editor (CE1) - pCMV-T7-FBYNV-nCas9-EcKlenow (JO610)

    Plasmid

    #208950

    Purpose

    A variant CE1 construct with FBYNV HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    FBYNV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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