We narrowed to 146,365 results for: ins;

1. pCMV-T7-SpCas9(RRTIMR)-P2A-EGFP (AHK149)

   Plasmid

   #223071

   Purpose

   pCMV and pT7 Human expression plasmid for SpCas9 enzyme with RRTIMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(RRTIMR)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9(RRTIMR)=D1135R, S1136R, G1218T, E1219I, R1…

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-T7-SpCas9(MRRCQR)-P2A-EGFP (RAS2823)

   Plasmid

   #223072

   Purpose

   pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRRCQR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(MRRCQR)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9(MRRCQR)=D1135M, S1136R, G1218R, E1219C, R1…

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-T7-SpCas9(IRAVQL)-P2A-EGFP (RTW3820)

   Plasmid

   #223073

   Purpose

   pCMV and pT7 Human expression plasmid for SpCas9 enzyme with IRAVQL amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(IRAVQL)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9(IRAVQL)=D1135I, S1136R, G1218A, E1219V, R1…

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-T7-SpCas9(SRSCMV)-P2A-EGFP (RTW3732)

   Plasmid

   #223074

   Purpose

   pCMV and pT7 Human expression plasmid for SpCas9 enzyme with SRSCMV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(SRSCMV)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9(SRSCMV)=D1135S, S1136R, G1218S, E1219C, R1…

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-T7-SpCas9(MKRCMV)-P2A-EGFP (AHK162)

   Plasmid

   #223075

   Purpose

   pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MKRCMV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(MKRCMV)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9(MKRCMV)=D1135M, S1136K, G1218R, E1219C, R1…

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-T7-SpCas9(MWNVML)-P2A-EGFP (AHK156)

   Plasmid

   #223076

   Purpose

   pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MWNVML amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(MWNVML)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9(MWNVML)=D1135M, S1136W, G1218N, E1219V, R1…

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-T7-SpCas9(MKKCMN)-P2A-EGFP (AHK129)

   Plasmid

   #223077

   Purpose

   pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MKKCMN amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(MKKCMN)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9(MKKCMN)=D1135M, S1136K, G1218K, E1219C, R1…

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-T7-SpCas9(KKKCMN)-P2A-EGFP (AHK131)

   Plasmid

   #223078

   Purpose

   pCMV and pT7 Human expression plasmid for SpCas9 enzyme with KKKCMN amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(KKKCMN)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9(KKKCMN)=D1135K, S1136K, G1218K, E1219C, R1…

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-T7-SpCas9(MRRWMR)-P2A-EGFP (RAS2816)

   Plasmid

   #223068

   Purpose

   pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRRWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(MRRWMR)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9(MRRWMR)=D1135M, S1136R, G1218R, E1219W, R1…

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-T7-SpCas9(ARGIMR)-P2A-EGFP (AHK147)

    Plasmid

    #223069

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with ARGIMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(ARGIMR)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(ARGIMR)=D1135A, S1136R, G1218G, E1219I, R1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-T7-SpCas9(ARTWGR)-P2A-EGFP (RAS2995)

    Plasmid

    #223070

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with ARTWGR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(ARTWGR)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(ARTWGR)=D1135A, S1136R, G1218T, E1219W, R1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLV-RnSyt1-sgRNA2-dCas9-KRAB-TagBFP2 (identifier AAAA-0246)

    Plasmid

    #202555

    Purpose

    Synaptotagmin-1 sgRNA2 with TagBFP2

    Depositor

    Frederic Meunier

    Article

    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

    Insert

    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCAGTACTCGCGTGCCTCGCACCGG) (Syt1 Rat)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLV-RnSyt1-sgRNA3-dCas9-KRAB-TagBFP2 (Identifier AAAA-0247)

    Plasmid

    #202556

    Purpose

    Synaptotagmin-1 sgRNA3 with TagBFP2

    Depositor

    Frederic Meunier

    Article

    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

    Insert

    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCTCCTCCTGCAGCGGCAGCATCGG) (Syt1 Rat)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLV-RnSyt1-sgRNA1-dCas9-KRAB-TagBFP2 (identifier AAAA-0245)

    Plasmid

    #202554

    Purpose

    Synaptotagmin-1 sgRNA1 with TagBFP2

    Depositor

    Frederic Meunier

    Article

    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

    Insert

    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ -CACCGCGTGCCTCGCACCGGTCCGCGG) (Syt1 R. norvegius (gRNA))
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p21(dPCNA)-mCerulean-NeoR

    Plasmid

    #215082

    Purpose

    Expressesp21(dPCNA)-mCerulean in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDKN1A (CDKN1A Human)
    

    Use

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    p21(dPCNA) denotes mutations M147A, D149A, F150A.

    Promoter

    CMV

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p21(RxL)-mCerulean-NeoR

    Plasmid

    #215081

    Purpose

    Expresses p21(RxL)-mCerulean in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDKN1A (CDKN1A Human)
    

    Use

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    p21(RxL) denotes mutations: R19A, R20A, L21A, F22A

    Promoter

    CMV

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p21(RxL)-mCerulean-PuroR

    Plasmid

    #215078

    Purpose

    Expresses p21(RxL)-mCerulean in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDKN1A (CDKN1A Human)
    

    Use

    Retroviral

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    p21(RxL) denotes mutations: R19A, R20A, L21A, F22A

    Promoter

    LTR

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p21(dPCNA)-mCerulean-PuroR

    Plasmid

    #215079

    Purpose

    Expresses p21(dPCNA)-mCerulean in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDKN1A (CDKN1A Human)
    

    Use

    Retroviral

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    p21(dPCNA) denotes mutations M147A, D149A, F150A.

    Promoter

    LTR

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-28b-T7-hAsCas12a-RVR(S542R/K548V/N552R)-NLS(nucleoplasmin)-6xHis (AAS1897)

    Plasmid

    #114074

    Purpose

    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-RVR(S542R/K548V/N552R) with C-terminal NLS and His-tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a-RVR (S542R/K548V/N552R) with C-terminal NLS and His-tag
    

    Use

    Tags

    NLS(nucleoplasmin) and 6xHis

    Expression

    Bacterial

    Mutation

    S542R, K548V and N552R

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBABEpuro-ERBB2 C299S

    Plasmid

    #40988

    Depositor

    Matthew Meyerson

    Article

    Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    C299S

    Promoter

    Available since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only