We narrowed to 5,897 results for: pcDNA 3.1

1. pMEH371 CD63 HA

   Plasmid

   #168220

   Purpose

   Mammalian expression of tetraspanin CD63 fused to a C-terminal HA tag

   Depositor

   Joshua Leonard

   Article

   Hung et al J Extracell Vesicles. 2016 May 13;5:3102

   Insert

   CD63 (CD63 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. GreenT-EC - Display - CyRFP1

   Plasmid

   #193943

   Purpose

   Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

   Depositor

   Oliver Griesbeck

   Article

   Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

   Insert

   GreenT-EC
   

   Use

   Tags

   Igk_secretion - HA-Flag and PDGFR

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GreenT-EC.b - Display - CyRFP1

   Plasmid

   #193944

   Purpose

   Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

   Depositor

   Oliver Griesbeck

   Article

   Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

   Insert

   GreenT-EC.b
   

   Use

   Tags

   Igk_secretion - HA-Flag and PDGFR

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GreenT-EC.c - Display - CyRFP1

   Plasmid

   #193945

   Purpose

   Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

   Depositor

   Oliver Griesbeck

   Article

   Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

   Insert

   GreenT-EC.c
   

   Use

   Tags

   Igk_secretion - HA-Flag and PDGFR

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GreenT-EC - GPI

   Plasmid

   #193946

   Purpose

   Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

   Depositor

   Oliver Griesbeck

   Article

   Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

   Insert

   GreenT-EC
   

   Use

   Tags

   GPI-anchor and Igk_secretion - HA-Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SnFR-gamma2

   Plasmid

   #165495

   Purpose

   Green fluorescent glutamate reporter fused to TARP gamma-2 for mammalian expression.

   Depositor

   Andrew Plested

   Article

   Hao et al eLife. 2023 Jan 9;12:e84029. doi: 10.755

   Insert

   iGluSnFR extracellular domain fused to Stargazin (gamma-2) via NETO2 TM domain (Cacng2 Rat, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CMV-FLAG-Core-R (pMO29)

   Plasmid

   #24480

   Depositor

   Melanie Ott

   Article

   Schwer et al J Virol. 2004 Aug . 78(15):7958-68.

   Insert

   Core
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 23, 2010

   Availability

   Academic Institutions and Nonprofits only

8. tau exon 9–12 FTDP-17 N279K splicing minigene

   Plasmid

   #122967

   Purpose

   expresses circular tau RNAs with the FTDP-17 N279K

   Depositor

   Stefan Stamm

   Article

   Welden et al Biochim Biophys Acta Mol Basis Dis. 2018

   Insert

   maps
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   FTDP-17 mutation

   Promoter

   Available since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ROZA YF

   Plasmid

   #64197

   Purpose

   Fluorescent Reporter for ZAP-70 tyrosine kinase activity-control YF mutant

   Depositor

   Annemarie Lellouch

   Article

   Randriamampita et al PLoS One. 2008 Jan 30;3(1):e1521. doi: 1

   Insert

   ROZA YF
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   tyrosine phosphorylatin site mutated to phenylala…

   Promoter

   CMV

   Available since

   April 23, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pMEH343 CD63 MS2 HA

    Plasmid

    #168217

    Purpose

    Mammalian expression of tetraspanin CD63 fused to the MS2 dimer and C-terminal HA tag

    Depositor

    Joshua Leonard

    Article

    Hung et al J Extracell Vesicles. 2016 May 13;5:3102

    Insert

    CD63 (CD63 Human)
    

    Use

    Tags

    HA and MS2 dimer

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMEH31 Lamp2b HA

    Plasmid

    #168208

    Purpose

    Mammalian expression of lysosome-associated membrane protein 2b fused to a C-terminal HA tag

    Depositor

    Joshua Leonard

    Article

    Hung et al J Extracell Vesicles. 2016 May 13;5:3102

    Insert

    Lamp2b (LAMP2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Emi LC

    Plasmid

    #113666

    Purpose

    For transient expression of Emicizumab sequence (CAS# 1610943-06-0, KEGG# D10821), IgG4 bispecific antibody targeting FIX(a) & FX(a). Must be co-expressed with anti-FIX(a) and anti-FX(a) Emi HC's.

    Depositor

    Robert Peters

    Article

    Leksa et al J Thromb Haemost. 2019 Mar 18. doi: 10.1

    Insert

    Emicizumab light chain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CMV-Cx35-Halo-C-in

    Plasmid

    #71511

    Purpose

    Mammalian expression plasmid encoding perch Cx35 with an internal HaloTag between amino acids 285 and 286 in the C-terminus.

    Depositor

    John O'Brien

    Article

    Wang et al J Cell Sci. 2015 Nov 1;128(21):3888-97.

    Insert

    Connexin 35
    

    Use

    Tags

    HaloTag (internal to sequence)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. RF-211-UB-Lyn

    Plasmid

    #79184

    Purpose

    Expression of the C-terminal fragment of Lyn kinase that is endogenously generated by caspase cleavage in mammalian cells

    Depositor

    Richard Fahlman

    Article

    Eldeeb et al Oncotarget. 2014 May 15;5(9):2714-22.

    Insert

    Lyn (LYN Human)
    

    Use

    Tags

    3x Flag and ubiquitin

    Expression

    Mammalian

    Mutation

    delta 1-18

    Promoter

    CMV

    Available since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Triple mutant

    Plasmid

    #63719

    Purpose

    Voltage sensing domain of Ciona with the A154D/R217Q/R229I mutations fused to super ecliptic pHlorin A227D

    Depositor

    Bradley Baker

    Article

    Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi

    Insert

    Triple mutant
    

    Use

    Tags

    Super ecliptic pHlorin A227D

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

16. CC2

    Plasmid

    #63714

    Purpose

    Voltage sensing domain of Ciona with the D136A/A154D/E183N/R223Q mutations fused to super ecliptic pHlorin A227D

    Depositor

    Bradley Baker

    Article

    Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi

    Insert

    CC2
    

    Use

    Tags

    Super ecliptic pHlorin A227D

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

17. UTS2R-DuET

    Plasmid

    #213386

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    UTS2R (UTS2R Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. nesprin TSmod 25

    Plasmid

    #125555

    Purpose

    Nesprin-2G force sensor with cerulean-F25-venus TSmodule

    Depositor

    Daniel Conway

    Article

    Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

    Insert

    mini-nesprin-2G (Syne2 Mouse)
    

    Use

    Tags

    cerulean, venus

    Expression

    Mammalian

    Mutation

    TSmod changed from mTFP to cerulean, flageliform …

    Promoter

    CMV

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. P2RY11-DuET

    Plasmid

    #213364

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    P2RY11 (P2RY11 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. OPN3-DuET

    Plasmid

    #213357

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    OPN3 (OPN3 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge