We narrowed to 18,356 results for: Tec

1. pX330A_FokI-1x2

   Plasmid

   #63602

   Purpose

   Expresses FokI-dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   FokI-dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG25-L-IFP-F[1] (clon 3)

   Plasmid

   #52899

   Purpose

   Plasmid template for PCR of cassettes for HR, used to introduce IFP F[1] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.

   Depositor

   Stephen Michnick

   Article

   Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

   Insert

   L-IFP-F[1]
   

   Use

   Vector for homologous recombination

   Tags

   Expression

   Mutation

   Promoter

   TEF promoter

   Available since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pAG32-L-IFP-F[2] (clon 3)

   Plasmid

   #52900

   Purpose

   Plasmid template for PCR of cassettes for HR, used to introduce IFP F[2] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.

   Depositor

   Stephen Michnick

   Article

   Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

   Insert

   L-IFP-F[2]
   

   Use

   Vector for homologous recombination

   Tags

   Expression

   Mutation

   Promoter

   TEF promoter

   Available since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pGWB602-mCherry-sfGFP

   Plasmid

   #213860

   Purpose

   For Agrobacterium transformation. mCherry-sfGFP under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

   Depositor

   Yutaka Kodama

   Article

   Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.

   Insert

   mCherry-sfGFP
   

   Use

   Tags

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2+-rasmKate

   Plasmid

   #105938

   Purpose

   in vitro transcription

   Depositor

   Caren Norden

   Article

   Weber et al Cell Rep. 2014 Apr 24;7(2):386-97. doi:

   Insert

   Ras
   

   Use

   Tags

   mKate

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBT271.359

   Plasmid

   #127845

   Purpose

   clone A3A CBEs by SapI Golden Gate

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   A3A
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CMV-iRFP

   Plasmid

   #64887

   Purpose

   Marking retinal neurons with infrared fluorescent proteins (iRFP) to preserve photoresponsiveness

   Depositor

   Tomomi Ichinose

   Article

   Fyk-Kolodziej et al Biotechniques. 2014 Nov 1;57(5):245-53.

   Insert

   iRFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLH-sgRNA1-boxB-MS2

   Plasmid

   #75396

   Purpose

   sgRNA1-boxB-MS2

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   sgRNA1-boxB-MS2
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCR1054

   Plasmid

   #71822

   Purpose

   Express Streptococcus pyogenes nCas9 (D10A) nickase carrying two C-terminal SV40 NLS

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

   Insert

   T7pr_6xHis-MBP-TEV-SPynCas9 (D10A)-2xNLS
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBT138b-pro1

    Plasmid

    #138513

    Purpose

    P3h

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    T7-RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTH1-SaSrtA-Q

    Plasmid

    #64980

    Purpose

    Expresses the E108Q mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Use

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, E108Q

    Promoter

    Available since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. Halo-Erk2 donor

    Plasmid

    #113104

    Purpose

    Donor plasmid for knock-in HaloTag fusing to the N-terminal of mouse Erk2 coding sequence

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    N terminal Erk2 donor region including homology arms and HaloTag
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ155

    Plasmid

    #69306

    Purpose

    Gateway entry vector with AteCas9D10A

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    AteCas9D10A (Arabidopsis codon optimized)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A mutation; NLS fusion

    Promoter

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBZS-YP_009701829

    Plasmid

    #198377

    Purpose

    Express YP_009701829 in bacterial cells.

    Depositor

    Takashi Ito

    Article

    Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

    Insert

    YP_009701829 (CVA-1_407L )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCfB6578

    Plasmid

    #106130

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUPD-Cyc1t (17-21)

    Plasmid

    #101700

    Purpose

    Cyc1t in domestication vector

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Insert

    GCTT-[terminator of S. cerevisiae YJR048W (190 bp)]-CGCT
    

    Use

    Yeast expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUPD-pTEF2 (01-11)

    Plasmid

    #101685

    Purpose

    pTEF2 in domestication vector

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Insert

    GGAG-[promoter of S. cerevisiae YBR118W (520bp)]-CCAT
    

    Use

    Yeast expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC-DEST-R3R4(R)

    Plasmid

    #141010

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTNS2

    Plasmid

    #87802

    Purpose

    For constructing NusA-SUMO-fusion proteins with N-terminal StrepII tag and C-terminal His tag using "blunt-end" cloning.

    Depositor

    Yu-Zhu Zhang

    Article

    Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167

    Type

    Empty backbone

    Use

    Tags

    6xHis and Strep II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pINTO-N-FH

    Plasmid

    #65924

    Purpose

    Inducible expression of proteins fused to an N-terminal Flag-HA tag (FH)

    Depositor

    Roberto Bonasio

    Article

    Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

    Type

    Empty backbone

    Use

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO2 (T-REx)

    Available since

    July 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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