Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 5,533 results for: PEP

Clear keywords
Showing: 4831 - 4860 of 5533 results

  1. pCEP4-SHIV327C-HuA10-QES.i09.c07

    Plasmid
    #123269
    Purpose
    Mammalian expression plasmid for Env from the SHIV327C-Hu A10 strain; QES mutant for enhanced presentation of quaternary epitopes
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    SHIV327C-Hu A10 Env
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Mutations V208M, …
    Promoter
    CMV
    Available Since
    March 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123269-plasmid-map-sequence-id-235232

    Enlarge

  2. pCEP4-DU422-QES.c12-754*

    Plasmid
    #123249
    Purpose
    Mammalian expression plasmid for Env from the DU422 HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (DU422) Env
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Premature stop co…
    Promoter
    CMV
    Available Since
    March 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123249-plasmid-map-sequence-id-235203

    Enlarge

  3. pCEP4-B41gp160-V200E-754*

    Plasmid
    #123244
    Purpose
    Mammalian expression plasmid for Env from the B41 HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (B41) Env
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Premature stop co…
    Promoter
    CMV
    Available Since
    March 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123244-plasmid-map-sequence-id-235194

    Enlarge

  4. pCEP4-191084-QES.i06.c07-754*

    Plasmid
    #123238
    Purpose
    Mammalian expression plasmid for Env from the 191084 B7-19 HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (191084 B7-19) Env
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Premature stop co…
    Promoter
    CMV
    Available Since
    March 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123238-plasmid-map-sequence-id-235189

    Enlarge

  5. pCEP4-25711-QES.c11-754*

    Plasmid
    #123218
    Purpose
    Mammalian expression plasmid for Env from the 25711 HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (25711) Env
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Premature stop co…
    Promoter
    CMV
    Available Since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    123218-plasmid-map-sequence-id-235160

    Enlarge

  6. Myc-gamma3/gamma2SIL

    Plasmid
    #121433
    Purpose
    GABAA receptor expression (chimeric rat gamma 3 subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminus
    Depositor
    Angel de Blas
    Insert
    GABRG3/GABRG2 SIL (Gabrg2, Gabrg3 Rat)
    Tags
    cMyc epitope EQKLISEEDL inserted between fourth a…
    Expression
    Mammalian
    Available Since
    March 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    121433-plasmid-map-sequence-id-237864

    Enlarge

  7. Myc-gamma1/gamma2SIL

    Plasmid
    #121432
    Purpose
    GABAA receptor expression (chimeric rat gamma 1 subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminus
    Depositor
    Angel de Blas
    Insert
    GABRG1/GABRG2 SIL (Gabrg1, Gabrg2 Rat)
    Tags
    cMyc epitope EQKLISEEDL inserted between fourth a…
    Expression
    Mammalian
    Available Since
    March 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    121432-plasmid-map-sequence-id-237859

    Enlarge

  8. pCEP4-HA-Flag-natT1R2 RR837-8GG

    Plasmid
    #113958
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Tags
    FLAG and Signal/leader sequence from influenza he…
    Expression
    Mammalian
    Mutation
    residues 22-839, R837G and R838G substitution
    Promoter
    cmv
    Available Since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113958-plasmid-map-sequence-id-215287

    Enlarge

  10. Ntng1.e-Fc-His

    Plasmid
    #72114
    Purpose
    Expresses the extracellular region of the Netrin G1, isoform e protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Ntng1.e (Ntng1 Mouse)
    Tags
    Fc-His
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72114-plasmid-map-sequence-id-131300

    Enlarge

  11. Ntng1.g-Fc-His

    Plasmid
    #72116
    Purpose
    Expresses the extracellular region of the Netrin G1, isoform g protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Ntng1.g (Ntng1 Mouse)
    Tags
    Fc-His
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72116-plasmid-map-sequence-id-131302

    Enlarge

  12. Ntng1.d-Fc-His

    Plasmid
    #72113
    Purpose
    Expresses the extracellular region of the Netrin G1, isoform d protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Ntng1.d (Ntng1 Mouse)
    Tags
    Fc-His
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72113-plasmid-map-sequence-id-131299

    Enlarge

  13. Ntng2.b-Fc-His

    Plasmid
    #72121
    Purpose
    Expresses the extracellular region of the Netrin G2, isoform b protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Ntng2.b (Ntng2 Mouse)
    Tags
    Fc-His
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72121-plasmid-map-sequence-id-131307

    Enlarge

  14. Ntng1.g-AP-His

    Plasmid
    #71990
    Purpose
    Expresses the extracellular region of the Netrin G1, isoform g protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Ntng1.g (Ntng1 Mouse)
    Tags
    AP-His
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71990-plasmid-map-sequence-id-131176

    Enlarge

  15. Ntng1.c-AP-His

    Plasmid
    #71986
    Purpose
    Expresses the extracellular region of the Netrin G1, isoform c protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Ntng1.c (Ntng1 Mouse)
    Tags
    AP-His
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    71986-plasmid-map-sequence-id-131172

    Enlarge

  16. Ntng1.f-AP-His

    Plasmid
    #71989
    Purpose
    Expresses the extracellular region of the Netrin G1, isoform f protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Ntng1.f (Ntng1 Mouse)
    Tags
    AP-His
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    71989-plasmid-map-sequence-id-131175

    Enlarge

  17. Ntng1.b-AP-His

    Plasmid
    #71985
    Purpose
    Expresses the extracellular region of the Netrin G1, isoform b protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Ntng1.b (Ntng1 Mouse)
    Tags
    AP-His
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    71985-plasmid-map-sequence-id-131171

    Enlarge

  18. Ntng1.h-AP-His

    Plasmid
    #71991
    Purpose
    Expresses the extracellular region of the Netrin G1, isoform h protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Ntng1.h (Ntng1 Mouse)
    Tags
    AP-His
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71991-plasmid-map-sequence-id-131177

    Enlarge

  19. Ntng1.e-AP-His

    Plasmid
    #71988
    Purpose
    Expresses the extracellular region of the Netrin G1, isoform e protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Ntng1.e (Ntng1 Mouse)
    Tags
    AP-His
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    71988-plasmid-map-sequence-id-131174

    Enlarge

  20. Ntng1.j-AP-His

    Plasmid
    #71993
    Purpose
    Expresses the extracellular region of the Netrin G1, isoform j protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Ntng1.j (Ntng1 Mouse)
    Tags
    AP-His
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    71993-plasmid-map-sequence-id-131179

    Enlarge

  22. pRS424-TUB2 - human TUBB4a Cterminal tail C127S, E435C

    Plasmid
    #60405
    Purpose
    Expression of chimeric yeast beta tubulin (TUB2) - human beta4a tubulin Cterminal tail (TUBB4a) C127S, E435C for crosslinking polyglutamate peptide
    Depositor
    Ron Vale
    Article
    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do
    Insert
    TUB2
    Expression
    Yeast
    Mutation
    TUB2 amino acids 429 - end, replaced with human T…
    Promoter
    GAL
    Available Since
    Jan. 26, 2015
    Availability
    Academic Institutions and Nonprofits only

  24. pTYB1-pLAC-N71

    Plasmid
    #48720
    Purpose
    Bacterial expression of Lacritin protein with 70 AA removed from the N-terminus
    Depositor
    Gordon Laurie
    Article
    Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6
    Insert
    Lacritin (LACRT Human)
    Tags
    Intein
    Expression
    Bacterial
    Mutation
    Deleted first 71 amino acids of mature lacritin w…
    Promoter
    T7
    Available Since
    Oct. 28, 2013
    Availability
    Academic Institutions and Nonprofits only

  26. MCHR1-DuET

    Plasmid
    #213337
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    MCHR1 (MCHR1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213337-plasmid-map-sequence-id-430912

    Enlarge

  27. MCHR2-DuET

    Plasmid
    #213338
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    MCHR2 (MCHR2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213338-plasmid-map-sequence-id-430867

    Enlarge

  28. ADORA2B-DuET

    Plasmid
    #213175
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    ADORA2B (ADORA2B Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213175-plasmid-map-sequence-id-430743

    Enlarge

  30. HCTR2-DuET

    Plasmid
    #213311
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HCTR2 (HCRTR2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213311-plasmid-map-sequence-id-430849

    Enlarge

Showing: 4831 - 4860 of 5533 results