We narrowed to 7,885 results for: Lif

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GC52 pEGFPN1 anti-Gephyrin nanobody

Plasmid

#135224

Purpose

Mammalian expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an intrabody

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Gephyrin (Human) recombinant llama nanobody GC52 (GPHN L. glama (llama))

Tags

6xHis-HA-EGFP

Expression

Mammalian

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV2-CAG-3xNLS-vsfGFP-0

Plasmid

#191097

Purpose

To express a bright green nanobody FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

Depositor

Murray Blackmore, Pantelis Tsoulfas

Article

Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

Insert

3xNLS-vsfGFP-0

Use

AAV

Tags

Green fluorescent protein bound to enhancer nanob…

Expression

Mammalian

Mutation

Optimized to Human codon usage

Promoter

CMV and CAG

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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SSpB-iRFP-p63DH

Plasmid

#176120

Purpose

Heterodimerization with iLID, RhoA activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

RHOGEF p63 (ARHGEF25 Human)

Expression

Mammalian

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Nav1.1 Na+ channel [K74/71R]

Plasmid

#128626

Purpose

Mammalian Expression Plasmid of anti-Nav1.1 Na+ channel (Rat). Derived from hybridoma K74/71.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Nav1.1 Na+ channel (Rat) recombinant mouse monoclonal antibody (Scn1a Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-Cre-cmlc2-TagBFP

Plasmid

#117792

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

Cre

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-VGlut3 [N34/34.2R]

Plasmid

#114494

Purpose

Mammalian Expression Plasmid of anti-VGlut3 (Rat). Derived from hybridoma N34/34.2.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-VGlut3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Slc17a8 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-SFFV-dCas9-P2A-EGFP

Plasmid

#188898

Purpose

Human lentiviral vector for expression of dCas9 with a C-terminal HA-2xNLS and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

dCas9

Use

Lentiviral

Tags

HA-2xNLS and P2A-GFP

Promoter

SFFV

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pGL4.15_pSOX17

Plasmid

#195496

Purpose

Firefly luciferase promoter expression vector driven only by the promoter element of human SOX17

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

Promoter region of SOX17 (SOX17 Human)

Tags

luc2-hPEST

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-Navbeta4 Na+ channel [N168/6.3R]

Plasmid

#114505

Purpose

Mammalian Expression Plasmid of anti-Navbeta4 Na+ channel (Rat). Derived from hybridoma N168/6.3.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Navbeta4 Na+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Scn4b Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph20-SNAPf-CCR5TC

Plasmid

#135537

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to SNAPf, CCR5TC zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Tags

SNAPf

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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HS69 pComb3xss anti-Homer1 nanobody

Plasmid

#134716

Purpose

Bacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Homer1 (Mouse) recombinant llama nanobody (Homer1 L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-H2B-Maroon-P2A-3xnls-Tq-Ca-FLITS

Plasmid

#145027

Purpose

Lentiviral vector for a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changes - co-expresses a nuclear marker

Depositor

Dorus Gadella

Article

van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

Insert

3xnls-Tq-Ca-FLITS

Use

Lentiviral

Tags

3xnls-Tq-Ca-FLITS and H2B-Maroon

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph18-eGFP-CCR5TC

Plasmid

#135529

Purpose

Encodes a specific PSD-95 binder (Xph18) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph18

Tags

eGFP

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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IC65 pEGFPN1 anti-IRSp53 nanobody

Plasmid

#135227

Purpose

Mammalian expression of anti-IRSp53 (Human) recombinant llama nanobody for use as an intrabody

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-IRSp53 (Human) recombinant llama nanobody IC65 (BAIAP2 L. glama (llama))

Tags

6xHis-HA-EGFP

Expression

Mammalian

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCDF RBP-FL P53

Plasmid

#174042

Purpose

Express Full length P53 in E.coli with cleavable expression/purification tag (ribose binding protein)

Depositor

Stewart Loh

Article

Blanden et al Elife. 2020 Dec 2;9. pii: 61487. doi: 10

Insert

P53 gene (TP53 Human)

Tags

Thermoanaerobacter Tencongenesis ribose binding p…

Expression

Bacterial

Promoter

T7

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pHR FGFR1-CD3ε-FusionRed

Plasmid

#188632

Purpose

Human FGFR1 labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensor

Depositor

Jared Toettcher

Article

Farahani et al Elife. 2023 May 22;12:e82863. doi: 10.75

Insert

FGFR1 (FGFR1 Synthetic, Human)

Use

Lentiviral

Tags

CD3ε-FusionRed

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET23a-H6-TEV-cAbl

Plasmid

#214234

Purpose

Bacterial expression of the kinase domain of cAbl with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screenings

Depositor

Neel Shah

Article

Shah et al Elife. 2018 Mar 16;7. pii: 35190. doi: 1

Insert

ABL1 Kinase domain (ABL1 Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph15-eGFP-CCR5TC

Plasmid

#135528

Purpose

Encodes a specific PSD-95 binder (Xph15) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph15

Tags

eGFP

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pPRISM-TagRFP-gcry-TagBFP

Plasmid

#117765

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-human LIC1 C-terminal half

Plasmid

#74599

Purpose

used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 389-523

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)

Tags

N-terminal GST and Strep II

Expression

Bacterial

Mutation

amino acids 389-523 of LIC1

Promoter

T7

Available Since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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