We narrowed to 38,991 results for: kan

1. pAcGFP1-N1-RvCAHS3-min-GS

   Plasmid

   #192463

   Purpose

   Expression of RvCAHS3-min-GS_linker-AcGFP1 in mammalian cells

   Depositor

   Takekazu Kunieda

   Article

   Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

   Insert

   CAHS3-min
   

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEE033

   Plasmid

   #176790

   Purpose

   Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

   Insert

   402
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEE043

   Plasmid

   #176800

   Purpose

   Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

   Insert

   412
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330 NLC-FKBP-Cas9

   Plasmid

   #162726

   Purpose

   Mammalian expression

   Depositor

   Amit Choudhary

   Article

   Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

   Insert

   FKBP F36V
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330 C-FKBP-Cas9

   Plasmid

   #162729

   Purpose

   Mammalian expression

   Depositor

   Amit Choudhary

   Article

   Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

   Insert

   FKBP F36V
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Gal4-HSF4b K294R

   Plasmid

   #118359

   Purpose

   This plasmid expresses amino-acids 200-493 of hHSF4b K294R mutant fused to the GAL-4 DNA binding domain. This construct cannot undergo sumoylation on K294.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

   Insert

   hHSF4b (HSF4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K294R

   Promoter

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. hHSF1 S307A

   Plasmid

   #118364

   Purpose

   This plasmid expresses human HSF1 S307A mutant.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

   Insert

   hHSF1 (HSF1 Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   S307A

   Promoter

   Available since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET30Xa-SBL1(160E,NoCys)

   Plasmid

   #66875

   Purpose

   Expresses a cysteine-free version of pET30Xa-SBL1(160E) (4Cys to Ser) with N-term Histag

   Depositor

   Betty Gaffney

   Article

   Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

   Insert

   soybean lipoxygenase-1 (S160E) with all Cys to Ser mutations
   

   Use

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   S160E; changed 4 (all) cysteines to serine

   Promoter

   T7lac

   Available since

   Aug. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pONL-C1_fibrillarin

   Plasmid

   #65710

   Purpose

   Expresses ONL-fibrillarin in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   fibrillarin (FBL Human)
   

   Use

   Luciferase

   Tags

   ONL

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pYNL-C1_fibrillarin

    Plasmid

    #65708

    Purpose

    Expresses YNL-fibrillarin in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    fibrillarin (FBL Human)
    

    Use

    Luciferase

    Tags

    YNL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. AB.pCCL.sin.cPPT.U6.miR-874-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46637

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-874 Decoy (MIR874, Mir874 Human, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

12. AB.pCCL.sin.cPPT.U6.miR-330-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46611

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-330 Decoy (MIR330, Mir330 Human, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

13. AB.pCCL.sin.cPPT.U6.miR-744-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46633

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-744 Decoy (MIR744, Mir744 Human, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

14. AB.pCCL.sin.cPPT.U6.miR-195-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46598

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-195 Decoy (MIR195, Mir195a Human, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pMVA2RBS035

    Plasmid

    #121051

    Purpose

    Expresses recombinant MVA pathway in Escherichia coli

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

    Inserts

    mvaE
    mvaS
    mvaK1
    mvaK2
    mvaD
    idi
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCK306

    Plasmid

    #110544

    Purpose

    rhaBAD, a rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria.

    Depositor

    John Heap

    Article

    Kelly et al ACS Synth Biol. 2018 Apr 2. doi: 10.1021

    Inserts

    PrhaBAD
    yfp
    rhaS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    N/A, PrhaBAD, and kanR promoter (upstream of kanR…

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA4-FLAG-Jhdm2a

    Plasmid

    #38136

    Depositor

    Toru Nakano, Toshinobu Nakamura

    Article

    Nakamura et al Nature. 2012 Jun 3;486(7403):415-9. doi:

    Insert

    Jhdm2a (Kdm3a Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    protein starts at aa115

    Promoter

    CMV

    Available since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA4-FLAG-Jhdm2a-H1122A

    Plasmid

    #38140

    Depositor

    Toru Nakano, Toshinobu Nakamura

    Article

    Nakamura et al Nature. 2012 Jun 3;486(7403):415-9. doi:

    Insert

    Jhdm2a-H1122A (Kdm3a Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    protein starts at aa115; H1122A

    Promoter

    CMV

    Available since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. hHSF1 S303/7A

    Plasmid

    #118365

    Purpose

    This plasmid expresses human HSF1 S303A and S307A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

    Insert

    hHSF1 (HSF1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    S303A and S307A

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMVA2

    Plasmid

    #119951

    Purpose

    Expresses recombinant MVA pathway in Escherichia coli

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

    Inserts

    mvaE
    mvaS
    mvaK1
    mvaK2
    mvaD
    idi
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A110G

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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