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We narrowed to 10,761 results for: T7

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Showing: 6481 - 6520 of 10761 results

  3. eSrtA-MT4

    Plasmid
    #200306
    Purpose
    Bacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT4. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtA
    Depositor
    Artur Krężel
    Article
    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.
    Insert
    eSrtA-LPETG-MT4 (MT4 Human)
    Tags
    His-tag and SUMO-tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    200306-plasmid-map-sequence-id-394863

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  4. eSrtA-MT3

    Plasmid
    #200305
    Purpose
    Bacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT3. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtA
    Depositor
    Artur Krężel
    Article
    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.
    Insert
    eSrtA-LPETG-MT3 (MT3 Human)
    Tags
    His-tag and SUMO-tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    200305-plasmid-map-sequence-id-394866

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  7. NeuA.pJL1

    Plasmid
    #199115
    Purpose
    In vitro expression of N-acylneuraminate cytodiylyltransfease (NeuA), which conjugates sialic acid and CTP to make activated sugar donor, CMP-sialic acid (see CSTI.PJL1 and PdST6.PJL1)
    Depositor
    Michael Jewett
    Article
    Thames et al ACS Synth Biol. 2023 Apr 11. doi: 10.102
    Insert
    N-acylneuraminate cytodiylyltransfease (NeuA)
    Tags
    Strep-tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    199115-plasmid-map-sequence-id-390797

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  8. Derp2.pJL1

    Plasmid
    #199116
    Purpose
    In vitro expression of Der p 2, a major common allergen from Dermatophagoides pteronyssinus (dust mite), with synthetic site for glycosylation encoded (GGNWTT)
    Depositor
    Michael Jewett
    Article
    Thames et al ACS Synth Biol. 2023 Apr 11. doi: 10.102
    Insert
    Der p 2 dust mite allergen
    Tags
    Glyctag, His-tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    199116-plasmid-map-sequence-id-390794

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  11. pSLS.539

    Plasmid
    #184958
    Purpose
    Test effect of a1/a2 length on editing fabH using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 12
    Expression
    Bacterial
    Mutation
    fabH donor G954A
    Promoter
    T7/lac
    Available Since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    184958-plasmid-map-sequence-id-381448

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  14. pENT-L3-eBra-L4

    Plasmid
    #186368
    Purpose
    Entry clone with ORF encoding Brachury cis-regulatory region flanked by Gateway recombination sequences
    Depositor
    Patrick Lemaire
    Article
    Roure et al PLoS One. 2007. 2(9):e916.
    Insert
    Brachury notochord enhancer
    Use
    Protein expression
    Promoter
    T7 promoter
    Available Since
    Nov. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    186368-plasmid-map-sequence-id-373444

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  16. pSLS.543

    Plasmid
    #184960
    Purpose
    Test effect of a1/a2 length on editing murF using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 12
    Expression
    Bacterial
    Mutation
    murF donor G1359A
    Promoter
    T7/lac
    Available Since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    184960-plasmid-map-sequence-id-376545

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  17. pSLS.545

    Plasmid
    #184962
    Purpose
    Test effect of a1/a2 length on editing priB using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 12
    Expression
    Bacterial
    Mutation
    priB donor G1069A
    Promoter
    T7/lac
    Available Since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184962-plasmid-map-sequence-id-376122

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  31. pCm N-term MBP SMT3 NikJC199U

    Plasmid
    #174363
    Purpose
    Expression plasmid with a ULP1 removable N-term MBP SUMO tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.
    Depositor
    Brandon Greene
    Article
    Caceres et al Methods Enzymol. 2022;662:119-141. doi:
    Insert
    nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
    Tags
    8xHis, MBP, SMT3, and TEV
    Expression
    Bacterial
    Mutation
    C199U
    Promoter
    T7
    Available Since
    Dec. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174363-plasmid-map-sequence-id-342843

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  33. pET29b-AviTag(K10A)-eGFP-dspB(E184Q)-6xHis

    Plasmid
    #175788
    Purpose
    Plasmid for overexpression of recombinant AviTagged(nonfunctional), His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.
    Depositor
    Mark Nitz
    Article
    Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12
    Insert
    Dispersin B
    Tags
    AviTag, Hexahistidine tag, and eGFP
    Expression
    Bacterial
    Mutation
    E184Q
    Promoter
    T7
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    175788-plasmid-map-sequence-id-345285

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  36. pENTR-D-kcnj16

    Plasmid
    #173146
    Purpose
    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.
    Depositor
    GuangJun Zhang
    Article
    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.
    Insert
    kcnj16a (kcnj16 Zebrafish)
    Use
    Pcr cloning vector
    Mutation
    Stop codon removed
    Promoter
    No promoter at 5 end; T7 promoter at 3 end.
    Available Since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    173146-plasmid-map-sequence-id-343336

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  38. Ash1

    Plasmid
    #169711
    Purpose
    Bacterial Expression of yeast Ash1 truncation (encodes amino acids 420-500)
    Depositor
    Tanja Mittag
    Article
    Martin et al J Am Chem Soc. 2016 Nov 30;138(47):15323
    Insert
    Ash1 (ASH1 Budding Yeast)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Truncation including encoding amino acids 420-500
    Promoter
    T7
    Available Since
    June 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    169711-plasmid-map-sequence-id-334782

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  39. pET11a-Z-N-wtBlc

    Plasmid
    #168258
    Purpose
    N-fragment of the wtBlc‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain wtBlc‐split-Zip protein
    Depositor
    Jens Meiler
    Article
    Bozhanova et al FEBS Lett. 2021 Jan;595(2):206-219. doi:
    Insert
    N-fragment of wtBlc + Leucine Zipper
    Tags
    His-tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168258-plasmid-map-sequence-id-332729

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Showing: 6481 - 6520 of 10761 results