Purpose
CMV and T7 promoter expression plasmid for human codon optimized ABE8e(V106W) A-to-G base editor with SpCas9(D10A) and P2A-EGFP
Insert
pCMV-T7-BPNLS-ABE8e(V106W)-SpCas9-BPNLS-P2A-EGFP
Use
CRISPR; In vitro transcription; t7 promoter
Tags
BPNLS and BPNLS-P2A-EGFP
Mutation
ABE8e mutations and V106W in TadA and nSpCas9(D10…
Available Since
Dec. 12, 2024
Availability
Academic Institutions and Nonprofits only