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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 17,767 results for: REV

Showing: 3241 - 3260 of 17767 results

  1. pGS1312

    Plasmid
    #63786
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCFP Strep-tag II followed by URA3 selection marker
    Depositor
    Bjoern Sander, Monika Golas
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eCFP-Strep-tag II followed by URA3
    Use
    E. coli cloning vector
    Tags
    eCFP-Strep-tag II
    Expression
    Mutation
    Promoter
    none
    Available since
    April 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    63786-plasmid-map-sequence-id-106475

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  4. pUC57-mitoLbNOX

    Plasmid
    #74448
    Purpose
    This plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.
    Depositor
    Vamsi Mootha
    Article
    Titov et al Science. 2016 Apr 8;352(6282):231-5. doi
    Insert
    mitoLbNOX
    Use
    Tags
    Flag tag with linker and mitochondrial targeting …
    Expression
    Mutation
    Human codon optimized
    Promoter
    Available since
    June 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    74448-plasmid-map-sequence-id-203966

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  5. pDGM6

    Plasmid
    #110660
    Purpose
    Adeno-Associated Virus (AAV) serotype 6; contains the AAV6 cap genes, AAV2 rep genes, and adenovirus helper genes. Good for in vivo transduction of muscle, lung, liver and other tissues.
    Depositor
    David Russell
    Article
    Gregorevic et al Nat Med. 2004 Aug;10(8):828-34. doi: 10.
    Insert
    AAV6 cap genes, AAV2 rep genes, adenovirus helper genes
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110660-plasmid-map-sequence-id-337382

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  8. pDuRCC-K

    Plasmid
    #81193
    Purpose
    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below., Please view…
    Promoter
    ROX3 and SNP52p
    Available since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    81193-plasmid-map-sequence-id-154419

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  10. pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB

    Plasmid
    #52889
    Purpose
    "CoinFLP-Gal4" - Expresses Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express Gal4, or FRT3-FRT3 pair to prevent Gal4 expression
    Depositor
    Iswar Hariharan
    Article
    Bosch et al Development. 2015 Feb 1;142(3):597-606.
    Insert
    Gal4 (GAL4 Budding Yeast)
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Jan. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    52889-plasmid-map-sequence-id-99556

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  13. pC-HYG-YR

    Plasmid
    #61765
    Purpose
    Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing hygromycin selectable marker on transfer DNA (TDNA).
    Depositor
    Ken Haynes
    Article
    Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do
    Inserts
    Hygromycin
    2 micron origin or replication and URA3 gene for S. cerevisiae
    Use
    Ternary vector for agrobacterium mediated transfo…
    Tags
    Expression
    Mutation
    Promoter
    trpC promoter
    Available since
    May 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    61765-plasmid-map-sequence-id-112551

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  14. pGMCK-T38S38-750-luxAB-DAS+4

    Plasmid
    #49518
    Purpose
    Kanamycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 site
    Depositor
    Dirk Schnappinger
    Article
    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.
    Insert
    T38S8-P750-luxAB-DAS+4
    Use
    Tags
    DAS+4 (AANDENYSENYADAS)
    Expression
    Bacterial
    Mutation
    Promoter
    P750
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    49518-plasmid-map-sequence-id-73896

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  15. pGS1021

    Plasmid
    #59575
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a TRP1 selection marker
    Depositor
    Bjoern Sander, Monika Golas
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    Twin-Strep tag optimized for PCR amplification followed by TRP1 selection marker
    Use
    E. coli cloning vector
    Tags
    Twin-Strep tag
    Expression
    Mutation
    Promoter
    None
    Available since
    Aug. 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    59575-plasmid-map-sequence-id-92981

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  19. pGMEH-T38M-750-luxAB-DAS+4

    Plasmid
    #49520
    Purpose
    Hygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); replicates episomally
    Depositor
    Dirk Schnappinger
    Article
    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.
    Insert
    T38M-P750-luxAB-DAS+4
    Use
    Tags
    DAS+4 (AANDENYSENYADAS)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    49520-plasmid-map-sequence-id-73899

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  20. pEasyG3_nat

    Plasmid
    #184918
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184918-plasmid-map-sequence-id-368408

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Showing: 3241 - 3260 of 17767 results