We narrowed to 4,337 results for: U6 gRNA

1. BoxB-MS2 adRNA (RAB7A-GAC site)

   Plasmid

   #170149

   Purpose

   AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript

   Depositor

   Prashant Mali

   Article

   Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

   Insert

   BoxB-RAB7A(GAC)-MS2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. MS2-adRNA (RAB7A-GAC site)

   Plasmid

   #170140

   Purpose

   AAV vector carrying a MS2 adRNA targeting the RAB7A transcript

   Depositor

   Prashant Mali

   Article

   Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

   Insert

   MS2-RAB7A(GAC)-MS2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1-TST-EGFP-GPIDAF

   Plasmid

   #213708

   Purpose

   For lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIDAF driven by an hPGK promoter.

   Depositor

   Qilai Huang

   Article

   Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

   Insert

   EGFP
   

   Use

   Tags

   Twin-strep-tag

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1-TST-EGFP-GPIBY55

   Plasmid

   #213707

   Purpose

   For lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIBY55 driven by an hPGK promoter.

   Depositor

   Qilai Huang

   Article

   Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

   Insert

   EGFP
   

   Use

   Tags

   Twin-strep-tag

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. hLig4 guide2 in pX458

   Plasmid

   #211536

   Purpose

   sgRNA-2 against Lig4

   Depositor

   Agnel Sfeir

   Article

   Brambati et al Science. 2023 Aug 11;381(6658):653-660.

   Insert

   sgRNA hLig4 (LIG4 Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   u6

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. hLig4 guide1 in pX458

   Plasmid

   #211535

   Purpose

   sgRNA-1 against Lig4

   Depositor

   Agnel Sfeir

   Article

   Brambati et al Science. 2023 Aug 11;381(6658):653-660.

   Insert

   sgRNA hLig4 (LIG4 Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   u6

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAf-CRISPR-ctfR1

   Plasmid

   #207992

   Purpose

   CRISPR vector used with pAf-CRISPR-phoA (#207991) to delete both aflatoxin and cyclopiazonic acid gene clusters of Aspergillus flavus

   Depositor

   Perng-Kuang Chang

   Article

   Chang et al Fungal Genet Biol. 2023 Dec 27;170:10386

   Insert

   ctfR1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Aspergillus flavus U6

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACBE-ASR (pXK-607)

   Plasmid

   #208822

   Purpose

   Universal Antibiotic Screening Reporter for adenine and cytosine base editors. Key Construct: CMV-PuroR*(ATAACG)-eGFP-hU6-gRNA.

   Depositor

   Kun Xu

   Article

   Ma et al J Biol Chem. 2022 Jul;298(7):102103. doi

   Type

   Empty backbone

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, U6

   Available since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACBE-FSR (pXK-1648)

   Plasmid

   #208821

   Purpose

   Universal Fluorescent Surrogate Reporter for adenine and cytosine base editors. Key Construct: CMV-mCherry-CMV-eGFP* (ATAACG)-hU6-gRNA.

   Depositor

   Kun Xu

   Article

   Ma et al J Biol Chem. 2022 Jul;298(7):102103. doi

   Type

   Empty backbone

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, U6

   Available since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. lenti-Cas9-sgHPRT1

    Plasmid

    #196713

    Purpose

    CRISPR-KO. WT-SpCas9 and sgRNA targeting HPRT1. Editing-competent cells can be selected with 6-TG

    Depositor

    Bernhard Schmierer

    Article

    Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

    Insert

    Cas9-T2A-BSD-U6-sgHPRT1
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a/hU6

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX459-sg-tdTomato166/892

    Plasmid

    #179919

    Purpose

    Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 166 and 892.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    tdTomato sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. PX552-mScn8a-3xHA-Syn-smFP-flag

    Plasmid

    #182563

    Purpose

    For mouse Nav1.6 knockin with 3xHA at the C-terminal

    Depositor

    James Zhe Liu

    Article

    Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

    Inserts

    mouse Scn8a gRNA and 3xHA donor DNA
    smFP-flag
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    EF1 and U6

    Available since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. PX552-mScn8a-HaloTag-V5-EF1-sfGFP

    Plasmid

    #182565

    Purpose

    For mouse Nav1.6 knockin with HaloTag-V5 at the C-terminal

    Depositor

    James Zhe Liu

    Article

    Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

    Inserts

    mouse Scn8a gRNA and HaloTag-V5 donor DNA
    sfGFP
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    EF1 and U6

    Available since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PX552-mScn8a-3xV5-EF1-smFP-flag

    Plasmid

    #182562

    Purpose

    For mouse Nav1.6 knockin with 3xV5 at the C-terminal

    Depositor

    James Zhe Liu

    Article

    Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

    Inserts

    mouse Scn8a gRNA and 3xV5 donor DNA
    smFP-flag
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    EF1 and U6

    Available since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJJF449_dpy-10_CDS_sg1

    Plasmid

    #163866

    Purpose

    sgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJR50.

    Depositor

    Nikolaus Rajewsky

    Article

    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

    Insert

    sgRNA targeting the coding sequence of dpy-10
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    R07E5.16 (U6)

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJJF328_sqt-3_3′UTR_sg2

    Plasmid

    #163867

    Purpose

    sgRNA targeting sqt-3 (3′UTR) in C. elegans. Backbone: pJJR50.

    Depositor

    Nikolaus Rajewsky

    Article

    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

    Insert

    sgRNA targeting the 3'UTR of sqt-3
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    R07E5.16 (U6)

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJJF496_dpy-10_CDS_sg6

    Plasmid

    #164267

    Purpose

    sgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJR50.

    Depositor

    Nikolaus Rajewsky

    Article

    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

    Insert

    sgRNA targeting the coding sequence of dpy-10
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    R07E5.16 (U6)

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJJF495_dpy-10_CDS_sg6

    Plasmid

    #164268

    Purpose

    sgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJF439.

    Depositor

    Nikolaus Rajewsky

    Article

    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

    Insert

    sgRNA targeting the coding sequence of dpy-10
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6 promoter from W05B2.8

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. sg2

    Plasmid

    #113967

    Purpose

    Single short guide RNA targeting TACCACATTTGTAGAGGTT

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    sg2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. sg3

    Plasmid

    #113968

    Purpose

    Single short guide RNA targeting CAATGTATCTTATCATGTC

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    sg3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge