We narrowed to 13,723 results for: cas9

1. pBZ191-pU6-sgBFP-CBh-sv40NLS-Cas9-NLS-T2A-mCherry

   Plasmid

   #170183

   Purpose

   Expression vector for a sgRNA against BFP and spCas9 linked to mCherry via a T2A peptide

   Depositor

   Hunter Fraser

   Article

   Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

   Insert

   sgBFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRP[2CRISPR]-mCherry/Hygro-hCas9-U6>{mdx left}-U6>{mdx right}

   Plasmid

   #184381

   Purpose

   This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23

   Depositor

   Ori Bar-Nur

   Article

   Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

   Insert

   Cas9, two gRNAs targeting dystrophin
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. 874U=ExuL-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

   Plasmid

   #159671

   Purpose

   Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

   Depositor

   Omar Akbari

   Article

   Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

   Insert

   ExuL-NLS-hSpCas9-T2A-GFP
   

   Use

   Tags

   T2A-GFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-nSpCas9(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES140)

   Plasmid

   #185490

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage nSpCas9(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

   Depositor

   Benjamin Kleinstiver, Raul Perez-Jimenez

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   human codon usage nSpCas9(H840A)-BPNLS-P2A-eGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-eGFP

   Expression

   Mammalian

   Mutation

   H840A

   Promoter

   CMV and T7

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMB1052:miniTol2-U6-(empty)SaCas9_gRNA-CAG-Cre-P2A-NeoR-WPRE

   Plasmid

   #168108

   Purpose

   Tol2 transposon vector of empty U6-S.aureus Cas9 gRNA cassette and CAG-driven Cre-NeoR

   Depositor

   Erich Jarvis

   Article

   Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. 1095=Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

   Plasmid

   #159673

   Purpose

   Plasmid supports Cas9 expression in both males and females, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

   Depositor

   Omar Akbari

   Article

   Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

   Insert

   Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40
   

   Use

   Tags

   T2A-GFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR GLIS3 TSS-guide3

   Plasmid

   #118175

   Purpose

   CRISPR-mediated repression of GLIS3. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CRY2-TSS-guide1

   Plasmid

   #125428

   Purpose

   CRISPR-mediated repression of CRY2. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CRY2-TSS-guide2

   Plasmid

   #125429

   Purpose

   CRISPR-mediated repression of CRY2. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4B-TSS-guide2

    Plasmid

    #125421

    Purpose

    CRISPR-mediated repression of C2CD4B. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4A-TSS-guide4

    Plasmid

    #125419

    Purpose

    CRISPR-mediated repression of C2CD4A. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR VPS13C-TSS-guide3

    Plasmid

    #125414

    Purpose

    CRISPR-mediated repression of VPS13C. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4AB-enh2-guide3

    Plasmid

    #125406

    Purpose

    CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs7163757 (C2CD4A/B GWAS locus)

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4AB-enh1-guide3

    Plasmid

    #125402

    Purpose

    CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs182222907 (C2CD4A/B GWAS locus)

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4AB-enh3-guide3

    Plasmid

    #125410

    Purpose

    CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs17205526 (C2CD4A/B GWAS locus)

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX601-AAV-CMV-NLS-SaCas9-NLS-3xHA-bGHpA;U6-Cre-1

    Plasmid

    #237890

    Purpose

    Cre-KO AAV vector#1 containing SaCas9 and its sgRNA targeting Cre

    Depositor

    Rui Lin

    Insert

    sgRNA-Cre
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pX601-AAV-CMV-NLS-SaCas9-NLS-3xHA-bGHpA;U6-Cre-2

    Plasmid

    #237891

    Purpose

    Cre-KO AAV vector#2 containing SaCas9 and its sgRNA targeting Cre

    Depositor

    Rui Lin

    Insert

    sgRNA-Cre
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX601-AAV-CMV-NLS-SaCas9-NLS-3xHA-bGHpA;U6-Cre-3

    Plasmid

    #237892

    Purpose

    Cre-KO AAV vector#3 containing SaCas9 and its sgRNA targeting Cre

    Depositor

    Rui Lin

    Insert

    sgRNA-Cre
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-eCas9-T2A-EGFP-U6-gRNA targeting Nrl-no ITR and f1 ori

    Plasmid

    #234737

    Purpose

    All-in-one CRISPR/Cas9 vector encodes high-fidelity eSpCas9 and a gRNA targeting Nrl, efficiently reprogramming rod precursors into cone-like cells in the mouse retina.

    Depositor

    Kirill Martemyanov

    Article

    Zhao et al Nat Commun. 2025 Apr 22;16(1):3768. doi:

    Insert

    Nrl (Nrl Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV hU6-VEGFR2#3 sgRNA Nestin-dCas9-KRAB-T2a-GFP

    Plasmid

    #196990

    Purpose

    Derived from pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP with KDR sgRNA

    Depositor

    Elizabeth Kirby

    Article

    Dause et al Mol Neurobiol. 2025 Mar 25. doi: 10.1007

    Insert

    VEGFR2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge